AB240317

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

GLUT3, SLC2A3, GLUT-3

4 Images

Supplier Data

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

This data was developed using ab191071, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (<a href='/en-us/products/primary-antibodies/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071'>ab191071</a>) at 1/10000 dilution

Lane 1:

SH-SHY5Y cell lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 54 kDa

false

Lab

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

This data was developed using ab191071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

We are unsure as to the identity of the band below 54kDa. This is not the target band.

All lanes:

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Observed band size: 54 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

This data was developed using ab191071, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW264.7 cell lysate at 10 µg

Lane 3:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 54 kDa

false

Lab

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

This data was developed using ab191071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Lane 1:

GST-tagged Recombinant Human SLC2A3(GLUT3) protein (Full length)

Lane 2:

GST tagged Recombinant Human SLC2A14(GLUT14) protein (aa51-105)

Lane 3:

MYC and DDK-tagged Recombinant Human SLC2A1(GLUT1) protein (Full length)

Lane 4:

MYC and DDK-tagged Recombinant Human SLC2A4(GLUT4) protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa,70 kDa

false

Exposure time: 3s

Unconjugated

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10508(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab240317 is the carrier-free version of ab191071.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUT3 also known as glucose transporter type 3 is a protein responsible for transporting glucose across cell membranes. It is part of the solute carrier family 2A with an approximate molecular weight of 54 kDa. GLUT3 expression is highest in the human brain especially in neurons allowing for efficient glucose uptake in these energy-demanding cells. The related transporter GLUT14 shares similarities with GLUT3 in function but is expressed at different levels in other tissues like the testes.

Biological function summary

GLUT3 facilitates the uptake of glucose which is important for the high metabolic demands of the brain. It does not require insulin for its function making it essential for energy metabolism under low glucose conditions. GLUT3 operates independently and is not reported as part of a larger protein complex. Its role ensures neurons maintain energy homeostasis for optimal functioning.

Pathways

GLUT3 plays an important role in the cellular glucose transport pathways. It greatly impacts the glycolysis pathway which is vital for energy production in neurons. Its function complements GLUT1 another glucose transporter by providing glucose access when neuronal energy demands rise. Both transporters collaboratively contribute to maintaining adequate glucose levels in the central nervous system allowing efficient cellular respiration.

Aberrations in GLUT3 function or expression associate with neurological disorders such as Alzheimer's disease. Altered glucose metabolism in the brain associated with impaired GLUT3 activity may exacerbate the development of such disorders. Moreover GLUT3 dysfunction links to certain types of cancer where altered glucose uptake and metabolism characterize the disease. GLUT3 affects glucose availability impacting cellular proliferation and survival pathways in tumorigenic processes with potential interaction with the protein PI3K known to play a role in cancer signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Facilitative glucose transporter (PubMed : 26176916, PubMed : 32860739, PubMed : 9477959). Can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed : 26176916, PubMed : 9477959). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed : 26176916, PubMed : 9477959). Does not mediate fructose transport (PubMed : 26176916, PubMed : 9477959). Required for mesendoderm differentiation (By similarity).

See full target information SLC2A3

Additional targets

SLC2A14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:105447 PubMed37949223

2023

Cardioprotective O-GlcNAc signaling is elevated in murine female hearts via enhanced O-GlcNAc transferase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Bhargavi Narayanan,Prithvi Sinha,Roger Henry,Russell A Reeves,Nazareno Paolocci,Mark J Kohr,Natasha E Zachara

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free (AB240317)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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Product promise