Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 31 publications.
Images
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img164885.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)")
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img17310.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)")
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img126121.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)")
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img70174.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)")
Publications
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- Nature cell biology 25:1821-18322023Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenna M Giafaglione,Preston D Crowell,Amelie M L Delcourt,Takao Hashimoto,Sung Min Ha,Aishwarya Atmakuri,Nicholas M Nunley,Rachel M A Dang,Mao Tian,Johnny A Diaz,Elisavet Tika,Marie C Payne,Deborah L Burkhart,Dapei Li,Nora M Navone,Eva Corey,Peter S Nelson,Neil Y C Lin,Cedric Blanpain,Leigh Ellis,Paul C Boutros,Andrew S GoldsteinPubMed 38049604
- The Journal of clinical investigation 133:2023GLUT3 promotes macrophage signaling and function via RAS-mediated endocytosis in atopic dermatitis and wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Min Yu,Jiawei Zhao,Eunice E Lee,Dohun Kim,Ruchika Mahapatra,Elysha K Rose,Zhiwei Zhou,Calvin Hosler,Abdullah El Kurdi,Jun-Yong Choe,E Dale Abel,Gerta Hoxhaj,Kenneth D Westover,Raymond J Cho,Jeffrey B Cheng,Richard C WangPubMed 37721853
- Translational andrology and urology 12:1497-15102023Proteomic approach towards identification of seminal fluid biomarkers from individuals with severe oligozoospermia, cryptozoospermia and non-obstructive azoospermia: a pilot study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarolina Nowicka-Bauer,Marzena Kamieniczna,Marta Olszewska,Maciej Krzysztof KurpiszPubMed 37969768
- Cellular oncology (Dordrecht) 46:1807-18202023Targeted CRISPR activation and knockout screenings identify novel doxorubicin transporters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Li,Minkang Tan,Shengnan Sun,Elena Stea,Baoxu PangPubMed 37523060
- Redox biology 58:1025432022SVCT2-mediated ascorbic acid uptake buffers stress responses via DNA hydroxymethylation reprogramming of S100 calcium-binding protein A4 gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian-Qian Han,Peng-Fei Wu,Yi-Heng Li,Yu Cao,Jian-Guo Chen,Fang WangPubMed 36436457
- Cellular and molecular life sciences : CMLS 79:2702022RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentin Jacquier,Delphine Gitenay,Samuel Fritsch,Sandrine Bonnet,Balázs Győrffy,Stéphan Jalaguier,Laetitia K Linares,Vincent Cavaillès,Catherine TeyssierPubMed 35501580
- Cell communication and signaling : CCS 20:402022An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zhang,Guo-Jun Geng,Jian-Guang Guo,Yan-Jun Mi,Xiao-Lei Zhu,Ning Li,Hong-Ming Liu,Jun-Feng Lin,Jian-Weng Wang,Guang Zhao,Guan-Zhi Ye,Bo-An Li,Qi-Cong Luo,Jie JiangPubMed 35346238
- Cell host & microbe 30:530-544.e62022Fungal sensing enhances neutrophil metabolic fitness by regulating antifungal Glut1 activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDe-Dong Li,Chetan V Jawale,Chunsheng Zhou,Li Lin,Giraldina J Trevejo-Nunez,Syed A Rahman,Steven J Mullet,Jishnu Das,Stacy G Wendell,Greg M Delgoffe,Michail S Lionakis,Sarah L Gaffen,Partha S BiswasPubMed 35316647
- BioMed research international 2022:33214092022Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Gexin Zhao,Xiaoyi Mi,Tonghong Xu,Xinmin Li,Bin LiuPubMed 35178446
- NPJ Parkinson's disease 8:102022Similar neuronal imprint and no cross-seeded fibrils in α-synuclein aggregates from MSA and Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlorent Laferrière,Stéphane Claverol,Erwan Bezard,Francesca De Giorgi,François IchasPubMed 35027576
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Facilitative glucose transporter (PubMed:26176916, PubMed:32860739, PubMed:9477959). Can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:26176916, PubMed:9477959). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:26176916, PubMed:9477959). Does not mediate fructose transport (PubMed:26176916, PubMed:9477959). Required for mesendoderm differentiation (By similarity).
Additional Targets
SLC2A14
Alternative names
GLUT3, SLC2A3, GLUT-3
Recommended products
Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 31 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10508(N)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLUT3 also known as glucose transporter type 3 is a protein responsible for transporting glucose across cell membranes. It is part of the solute carrier family 2A with an approximate molecular weight of 54 kDa. GLUT3 expression is highest in the human brain especially in neurons allowing for efficient glucose uptake in these energy-demanding cells. The related transporter GLUT14 shares similarities with GLUT3 in function but is expressed at different levels in other tissues like the testes.
- Biological function summary
GLUT3 facilitates the uptake of glucose which is important for the high metabolic demands of the brain. It does not require insulin for its function making it essential for energy metabolism under low glucose conditions. GLUT3 operates independently and is not reported as part of a larger protein complex. Its role ensures neurons maintain energy homeostasis for optimal functioning.
- Pathways
GLUT3 plays an important role in the cellular glucose transport pathways. It greatly impacts the glycolysis pathway which is vital for energy production in neurons. Its function complements GLUT1 another glucose transporter by providing glucose access when neuronal energy demands rise. Both transporters collaboratively contribute to maintaining adequate glucose levels in the central nervous system allowing efficient cellular respiration.
- Associated diseases and disorders
Aberrations in GLUT3 function or expression associate with neurological disorders such as Alzheimer's disease. Altered glucose metabolism in the brain associated with impaired GLUT3 activity may exacerbate the development of such disorders. Moreover GLUT3 dysfunction links to certain types of cancer where altered glucose uptake and metabolism characterize the disease. GLUT3 affects glucose availability impacting cellular proliferation and survival pathways in tumorigenic processes with potential interaction with the protein PI3K known to play a role in cancer signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img164885.jpg "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)")
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)
Blocking and dilution buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution
Lane 1: GST-tagged Recombinant Human SLC2A3(GLUT3) protein (Full length)
Lane 2: GST tagged Recombinant Human SLC2A14(GLUT14) protein (aa51-105)
Lane 3: MYC and DDK-tagged Recombinant Human SLC2A1(GLUT1) protein (Full length)
Lane 4: MYC and DDK-tagged Recombinant Human SLC2A4(GLUT4) protein (Full length)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 38 kDa, 70 kDa
Exposure time: 3s
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img17310.jpg "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)")
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
We are unsure as to the identity of the band below 54kDa. This is not the target band.
All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat kidney tissue lysate at 20 µg
Lane 8: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 54 kDa
Exposure time: 15s
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img126121.jpg "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)")
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)
All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/10000 dilution
Lane 1: SH-SHY5Y cell lysate at 20 µg
Lane 2: U87-MG cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Observed band size: 54 kDa
![Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut3-glut14-antibody-epr10508n-n-terminal-ab191071--western-blot-img70174.jpg "Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)")
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)
All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: RAW264.7 cell lysate at 10 µg
Lane 3: NIH 3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Observed band size: 54 kDa
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