AB191071

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal

Name: Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) | Abcam
Brand: Abcam Limited
SKU: AB191071
Price: 220 USD
Availability: InStock
Rating: 5 (6 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(6 Reviews)

(45 Publications)

Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 45 publications.

View Alternative Names

GLUT3, SLC2A3, GLUT-3, GLUT14, SLC2A14, Glucose transporter type 14, GLUT-14

4 Images

Supplier Data

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

All lanes:

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/10000 dilution

Lane 1:

SH-SHY5Y cell lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 54 kDa

false

Lab

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

We are unsure as to the identity of the band below 54kDa. This is not the target band.

All lanes:

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Observed band size: 54 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

All lanes:

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW264.7 cell lysate at 10 µg

Lane 3:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 54 kDa

false

Lab

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution

Lane 1:

GST-tagged Recombinant Human SLC2A3(GLUT3) protein (Full length)

Lane 2:

GST tagged Recombinant Human SLC2A14(GLUT14) protein (aa51-105)

Lane 3:

MYC and DDK-tagged Recombinant Human SLC2A1(GLUT1) protein (Full length)

Lane 4:

MYC and DDK-tagged Recombinant Human SLC2A4(GLUT4) protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa,70 kDa

false

Exposure time: 3s

Carrier free

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10508(N)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUT3 also known as glucose transporter type 3 is a protein responsible for transporting glucose across cell membranes. It is part of the solute carrier family 2A with an approximate molecular weight of 54 kDa. GLUT3 expression is highest in the human brain especially in neurons allowing for efficient glucose uptake in these energy-demanding cells. The related transporter GLUT14 shares similarities with GLUT3 in function but is expressed at different levels in other tissues like the testes.

Biological function summary

GLUT3 facilitates the uptake of glucose which is important for the high metabolic demands of the brain. It does not require insulin for its function making it essential for energy metabolism under low glucose conditions. GLUT3 operates independently and is not reported as part of a larger protein complex. Its role ensures neurons maintain energy homeostasis for optimal functioning.

Pathways

GLUT3 plays an important role in the cellular glucose transport pathways. It greatly impacts the glycolysis pathway which is vital for energy production in neurons. Its function complements GLUT1 another glucose transporter by providing glucose access when neuronal energy demands rise. Both transporters collaboratively contribute to maintaining adequate glucose levels in the central nervous system allowing efficient cellular respiration.

Aberrations in GLUT3 function or expression associate with neurological disorders such as Alzheimer's disease. Altered glucose metabolism in the brain associated with impaired GLUT3 activity may exacerbate the development of such disorders. Moreover GLUT3 dysfunction links to certain types of cancer where altered glucose uptake and metabolism characterize the disease. GLUT3 affects glucose availability impacting cellular proliferation and survival pathways in tumorigenic processes with potential interaction with the protein PI3K known to play a role in cancer signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Facilitative glucose transporter (PubMed : 26176916, PubMed : 32860739, PubMed : 9477959). Can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed : 26176916, PubMed : 9477959). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed : 26176916, PubMed : 9477959). Does not mediate fructose transport (PubMed : 26176916, PubMed : 9477959). Required for mesendoderm differentiation (By similarity).

See full target information SLC2A3

Additional targets

SLC2A14

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1608400 PubMed40837190

2025

Epigenetic regulation of the respiratory chain by a mitochondrial distress-related redox signal.

Applications

Unspecified application

Species

Unspecified reactive species

Marius W Baeken,Ankush Borlepawar,Philipp Kötzner,Holger Richly,Christian Behl,Bernd Moosmann,Parvana Hajieva

Cell death discovery 11:382 PubMed40813374

2025

GLUT3 enhances chemosensitivity in glioblastoma by transporting temozolomide and capecitabine.

Applications

Unspecified application

Species

Unspecified reactive species

Honglin Diao,Yuxin Sun,Xiaojia Zhou,Qikai Wang,Mingyue Wang,Keyu Chen,Zhihua Huang,Jianlei Wei,Zeping Li,Yaxin Lou,Zebin Mao,Wenhua Yu

Nature communications 16:4190 PubMed40328784

2025

Astrocytic GLUT1 deletion in adult mice enhances glucose metabolism and resilience to stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Laetitia Thieren,Henri S Zanker,Jeanne Droux,Urvashi Dalvi,Matthias T Wyss,Rebecca Waag,Pierre-Luc Germain,Lukas M von Ziegler,Zoe J Looser,Ladina Hösli,Luca Ravotto,E Dale Abel,Johannes Bohacek,Susanne Wegener,L Felipe Barros,Mohamad El Amki,Bruno Weber,Aiman S Saab

Molecular medicine (Cambridge, Mass.) 31:108 PubMed40114059

2025

Spatiotemporal diversity in molecular and functional abnormalities in the mdx dystrophic brain.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Pomeroy,Malgorzata Borczyk,Maria Kawalec,Jacek Hajto,Emma Carlson,Samuel Svärd,Suraj Verma,Eric Bareke,Anna Boratyńska-Jasińska,Dorota Dymkowska,Alvaro Mellado-Ibáñez,David Laight,Krzysztof Zabłocki,Annalisa Occhipinti,Loydie Majewska,Claudio Angione,Jacek Majewski,Gennady G Yegutkin,Michal Korostynski,Barbara Zabłocka,Dariusz C Górecki

Journal of pineal research 76:e70002 PubMed39539049

2024

Melatonin Protects Against Cocaine-Induced Blood-Brain Barrier Dysfunction and Cognitive Impairment by Regulating miR-320a-Dependent GLUT1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Yi Wei,Hui Liu,Yuan Li,Dan Zhao,Bo Wang,Hui-Jie Wang,Li Wang,Kang-Ji Wang,Jin-Li Yue,Hong-Yan Zhang,Tian-Yue Li,Yi-Jue Miao,Kai-Li Wang,Pai-Ge Tong,Zhuo Zhang,Ze-Ye Li,Zheng Shi,Jia-Yuan Yao,Dong-Xin Liu,Wen-Gang Fang,Bo Li,De-Shu Shang,Yuan Lyu,Hong-Zan Sun,Wei-Dong Zhao,Yu-Hua Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2407346 PubMed39499780

2024

Hyperglycemia-Enhanced Neutrophil Extracellular Traps Drive Mucosal Immunopathology at the Oral Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Weimin Lin,Kexin Lei,Hui Wang,Xiaohan Zhang,Shuang Jiang,Danting Zhang,Wen Wang,Shuqin Cao,Yuyu Li,Bo Yu,Yuan Wang,Qi Yin,Quan Yuan

Gut microbes 16:2395907 PubMed39262376

2024

Fecal microbiota transplantation derived from mild cognitive impairment individuals impairs cerebral glucose uptake and cognitive function in wild-type mice: and TXNIP-GLUT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Ling Hao,Kexin Yang,Wenjing Feng,Zhiting Guo,Miao Liu,Rong Xiao

Nature neuroscience 27:1934-1944 PubMed39251890

2024

Oligodendroglial fatty acid metabolism as a central nervous system energy reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Ebrahim Asadollahi,Andrea Trevisiol,Aiman S Saab,Zoe J Looser,Payam Dibaj,Reyhane Ebrahimi,Kathrin Kusch,Torben Ruhwedel,Wiebke Möbius,Olaf Jahn,Jun Yup Lee,Anthony S Don,Michelle-Amirah Khalil,Karsten Hiller,Myriam Baes,Bruno Weber,E Dale Abel,Andrea Ballabio,Brian Popko,Celia M Kassmann,Hannelore Ehrenreich,Johannes Hirrlinger,Klaus-Armin Nave

Cell reports. Medicine 5:101400 PubMed38307031

2024

Inhibition of CD38 enzymatic activity enhances CAR-T cell immune-therapeutic efficacy by repressing glycolytic metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Huang,Mi Shao,Xinyi Teng,Xiaohui Si,Longyuan Wu,Penglei Jiang,Lianxuan Liu,Bohan Cai,Xiujian Wang,Yingli Han,Youqin Feng,Kai Liu,Zhaoru Zhang,Jiazhen Cui,Mingming Zhang,Yongxian Hu,Pengxu Qian,He Huang

Journal of advanced research 65:125-136 PubMed38070595

2023

The NLRP3 molecule influences the therapeutic effects of mesenchymal stem cells through Glut1-mediated energy metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Jingrou Chen,Shujuan Xie,Dongbo Qiu,Maosheng Xie,Mengye Wu,Xiaoping Li,Xiaoran Zhang,Qili Wu,Yi Xiong,Changyou Wu,Jie Ren,Yanwen Peng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (45)

Product promise