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Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 31 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Target data

Function

Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916).

Alternative names

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Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 31 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10508(N)

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

GLUT3 facilitates the uptake of glucose which is important for the high metabolic demands of the brain. It does not require insulin for its function making it essential for energy metabolism under low glucose conditions. GLUT3 operates independently and is not reported as part of a larger protein complex. Its role ensures neurons maintain energy homeostasis for optimal functioning.

Activity summary

GLUT3 also known as glucose transporter type 3 is a protein responsible for transporting glucose across cell membranes. It is part of the solute carrier family 2A with an approximate molecular weight of 54 kDa. GLUT3 expression is highest in the human brain especially in neurons allowing for efficient glucose uptake in these energy-demanding cells. The related transporter GLUT14 shares similarities with GLUT3 in function but is expressed at different levels in other tissues like the testes.

Pathways

GLUT3 plays an important role in the cellular glucose transport pathways. It greatly impacts the glycolysis pathway which is vital for energy production in neurons. Its function complements GLUT1 another glucose transporter by providing glucose access when neuronal energy demands rise. Both transporters collaboratively contribute to maintaining adequate glucose levels in the central nervous system allowing efficient cellular respiration.

Associated diseases and disorders

Aberrations in GLUT3 function or expression associate with neurological disorders such as Alzheimer's disease. Altered glucose metabolism in the brain associated with impaired GLUT3 activity may exacerbate the development of such disorders. Moreover GLUT3 dysfunction links to certain types of cancer where altered glucose uptake and metabolism characterize the disease. GLUT3 affects glucose availability impacting cellular proliferation and survival pathways in tumorigenic processes with potential interaction with the protein PI3K known to play a role in cancer signaling pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail

    Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071) at 1/1000 dilution

    Lane 1: GST-tagged Recombinant Human SLC2A3(GLUT3) protein (Full length)

    Lane 2: GST tagged Recombinant Human SLC2A14(GLUT14) protein (aa51-105)

    Lane 3: MYC and DDK-tagged Recombinant Human SLC2A1(GLUT1) protein (Full length)

    Lane 4: MYC and DDK-tagged Recombinant Human SLC2A4(GLUT4) protein (Full length)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 38 kDa, 70 kDa

    Exposure time: 3s

    This data was developed using ab191071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail

    Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    We are unsure as to the identity of the band below 54kDa. This is not the target band.

    All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Lane 6: Rat heart tissue lysate at 20 µg

    Lane 7: Rat kidney tissue lysate at 20 µg

    Lane 8: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Observed band size: 54 kDa

    Exposure time: 15s

  • Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail

    Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)

    All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071) at 1/10000 dilution

    Lane 1: SH-SHY5Y cell lysate at 20 µg

    Lane 2: U87-MG cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Observed band size: 54 kDa

  • Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071), expandable thumbnail

    Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)

    All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: RAW264.7 cell lysate at 10 µg

    Lane 3: NIH 3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Observed band size: 54 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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