Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)

Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. Suitable for Dot, WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	WB	IHC-P	ICC/IF	IP	Flow Cyt (Intra)
Human	Expected	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Tested	Expected	Expected	Expected
Rat	Expected	Tested	Tested	Expected	Expected	Expected
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information SLC2A3

Function

Facilitative glucose transporter (PubMed:26176916, PubMed:32860739, PubMed:9477959). Can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:26176916, PubMed:9477959). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:26176916, PubMed:9477959). Does not mediate fructose transport (PubMed:26176916, PubMed:9477959). Required for mesendoderm differentiation (By similarity).

Additional Targets

SLC2A14

Alternative names

GLUT3, SLC2A3, GLUT-3

Recommended products

Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. Suitable for Dot, WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28481-54
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GLUT3 also known as glucose transporter type 3 is a protein responsible for transporting glucose across cell membranes. It is part of the solute carrier family 2A with an approximate molecular weight of 54 kDa. GLUT3 expression is highest in the human brain especially in neurons allowing for efficient glucose uptake in these energy-demanding cells. The related transporter GLUT14 shares similarities with GLUT3 in function but is expressed at different levels in other tissues like the testes.

Biological function summary

GLUT3 facilitates the uptake of glucose which is important for the high metabolic demands of the brain. It does not require insulin for its function making it essential for energy metabolism under low glucose conditions. GLUT3 operates independently and is not reported as part of a larger protein complex. Its role ensures neurons maintain energy homeostasis for optimal functioning.

Pathways

GLUT3 plays an important role in the cellular glucose transport pathways. It greatly impacts the glycolysis pathway which is vital for energy production in neurons. Its function complements GLUT1 another glucose transporter by providing glucose access when neuronal energy demands rise. Both transporters collaboratively contribute to maintaining adequate glucose levels in the central nervous system allowing efficient cellular respiration.

Associated diseases and disorders

Aberrations in GLUT3 function or expression associate with neurological disorders such as Alzheimer's disease. Altered glucose metabolism in the brain associated with impaired GLUT3 activity may exacerbate the development of such disorders. Moreover GLUT3 dysfunction links to certain types of cancer where altered glucose uptake and metabolism characterize the disease. GLUT3 affects glucose availability impacting cellular proliferation and survival pathways in tumorigenic processes with potential interaction with the protein PI3K known to play a role in cancer signaling pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Glucose Transporter GLUT3 + GLUT14 Western blot staining using rabbit Anti-Glucose Transporter GLUT3 + GLUT14 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 33061855, PMID: 25526803, PMID: 34031595).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
Exposure time: Lane 1: 48 seconds, lane 2: 10 seconds.
All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 49-70 kDa, 124 kDa
Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Glucose Transporter GLUT3 + GLUT14 was immunoprecipitated from 0.35 mg SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab315168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315168 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab315168 IP in SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315168 in SW480 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/30 dilution
All lanes: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle (PMID: 3170580).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 33061855, PMID: 25526803, PMID: 34031595).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Human skeletal muscle lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 49 kDa, 36 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on human skeletal muscle.The section was incubated with ab315168 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/100 (5.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing membranous staining in SW480 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml)dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on rat skeletal muscle. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on mouse skeletal muscle. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat testis. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human testis. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle (PMID: 3170580).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 33061855, PMID: 25526803, PMID: 34031595).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/1000 dilution
Lane 1: Mouse cerebellum tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat skeletal muscle lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 49 kDa, 36 kDa
Exposure time: 180s
Dot Blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168)
Dot blot analysis of Glucose Transporter GLUT3 + GLUT14 using ab315168 at 1:1000 (0.52 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: 293T cells transfected with an empty vector containing a His-tag whole cell lysate
Lane 2: 293T cells transfected with a human SLC2A3 expression vector containing a His-tag whole cell lysate
Lane 3: 293T cells transfected with a human SLC2A14 expression vector containing a His-tag whole cell lysate
Lane 4: 293T cells transfected with a human SLC2A2 expression vector containing a His-tag whole cell lysate
Exposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody reacts with human SLC2A3 and SLC2A14 but not SLC2A2.