Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
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(1 Publication)
Rabbit Recombinant Monoclonal Glucose Transporter GLUT3 antibody. Suitable for Dot, WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples. Cited in 1 publication.
View Alternative Names
GLUT3, SLC2A3, GLUT-3, GLUT14, SLC2A14, Glucose transporter type 14, GLUT-14
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human testis. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue : No staining on human skeletal muscle.The section was incubated with ab315168 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/100 (5.2 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing membranous staining in SW480 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml)dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Glucose Transporter GLUT3 + GLUT14 was immunoprecipitated from 0.35 mg SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab315168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315168 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab315168 IP in SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315168 in SW480 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/30 dilution
All lanes:
SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 10s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat testis. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue : No staining on mouse skeletal muscle. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Glucose Transporter GLUT3 + GLUT14 with ab315168 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue : No staining on rat skeletal muscle. The section was incubated with ab315168 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- WB
Supplier Data
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33061855, PMID : 25526803, PMID : 34031595).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
Exposure time : Lane 1 : 48 seconds, lane 2 : 10 seconds.
All lanes:
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/1000 dilution
Lane 1:
HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 49-70 kDa,124 kDa
false
- WB
Supplier Data
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Low expression : skeletal muscle (PMID : 3170580).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33061855, PMID : 25526803, PMID : 34031595).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/1000 dilution
Lane 1:
Human hippocampus tissue lysate at 20 µg
Lane 2:
Human cerebellum tissue lysate at 20 µg
Lane 3:
Human skeletal muscle lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 49 kDa,36 kDa
false
Exposure time: 3s
- Dot
Supplier Data
Dot Blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Dot blot analysis of Glucose Transporter GLUT3 + GLUT14 using ab315168 at 1 : 1000 (0.52 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.
Lane 1 : 293T cells transfected with an empty vector containing a His-tag whole cell lysate
Lane 2 : 293T cells transfected with a human SLC2A3 expression vector containing a His-tag whole cell lysate
Lane 3 : 293T cells transfected with a human SLC2A14 expression vector containing a His-tag whole cell lysate
Lane 4 : 293T cells transfected with a human SLC2A2 expression vector containing a His-tag whole cell lysate
Exposure time : 180 seconds.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody reacts with human SLC2A3 and SLC2A14 but not SLC2A2.
- WB
Supplier Data
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (AB315168)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Low expression : skeletal muscle (PMID : 3170580).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33061855, PMID : 25526803, PMID : 34031595).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] (ab315168) at 1/1000 dilution
Lane 1:
Mouse cerebellum tissue lysate at 20 µg
Lane 2:
Mouse brain tissue lysate at 20 µg
Lane 3:
Mouse skeletal muscle lysate at 20 µg
Lane 4:
Rat brain tissue lysate at 20 µg
Lane 5:
Rat skeletal muscle lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 49 kDa,36 kDa
false
Exposure time: 180s
Related conjugates and formulations (2)
-
Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54] - BSA and Azide free
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR28481-54]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GLUT3 facilitates the uptake of glucose which is important for the high metabolic demands of the brain. It does not require insulin for its function making it essential for energy metabolism under low glucose conditions. GLUT3 operates independently and is not reported as part of a larger protein complex. Its role ensures neurons maintain energy homeostasis for optimal functioning.
Pathways
GLUT3 plays an important role in the cellular glucose transport pathways. It greatly impacts the glycolysis pathway which is vital for energy production in neurons. Its function complements GLUT1 another glucose transporter by providing glucose access when neuronal energy demands rise. Both transporters collaboratively contribute to maintaining adequate glucose levels in the central nervous system allowing efficient cellular respiration.
Product protocols
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Target data
Additional targets
Publications (1)
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Heliyon 10:e34425 PubMed39082040
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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