AB35826

Anti-Glucose Transporter GLUT4 antibody [1F8]

Name: Anti-Glucose Transporter GLUT4 antibody [1F8] (ab35826) | Abcam
Brand: Abcam Limited
SKU: AB35826

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(17 Publications)

Mouse Monoclonal Glucose Transporter GLUT4 antibody. Suitable for WB and reacts with Mouse samples. Cited in 17 publications. Immunogen corresponding to Cell preparation containing Slc2a4 protein.

View Alternative Names

Glut4, Slc2a4, GT2, GLUT-4

1 Images

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Western blot - Anti-Glucose Transporter GLUT4 antibody [1F8] (AB35826)

3T3-L1 lysate probed with Mouse anti Rat GLUT4 (ab35826) followed by Goat anti Mouse IgG : Dylight^®800. Imaged using the LiCor Odyssey ®

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 antibody [1F8] (ab35826)

Predicted band size: 54 kDa

false

Carrier free

Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free
Unconjugated

Anti-ENPP2/ATX antibody [1F8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F8

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Cell preparation containing Slc2a4 protein. The exact immunogen used to generate this antibody is proprietary information.

P19357

Epitope

This antibody has been shown to recognise an epitope in the cytoplasmic portion of the molecule.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>5 μg/ml of antibody will detect Glucose Transporter protein contained in 50-200 μg of solubilised membrane.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose transporter GLUT4 also known as GLUT-4 or GLUT 4 transporter plays an important role in glucose uptake in cells. It is an insulin-responsive class I facilitated glucose transporter. GLUT4 predominantly expresses in insulin-sensitive tissues such as adipose tissue and skeletal and cardiac muscles. The functional transport mechanism facilitates glucose entry into the cell by translocating from intracellular vesicles to the plasma membrane in response to insulin stimulation. The molecular weight of GLUT4 is about 50 kDa and this property is often observed in techniques such as GLUT4 western blot where it aids in the identification of the transporter.

Biological function summary

GLUT4 helps regulate blood sugar levels through its control over glucose uptake in muscle and adipose tissues. As an important component of the insulin-regulated glucose transport system GLUT4 responds to increased insulin levels by moving to the cell's surface aiding in glucose transportation into the cell. This adaptive response reduces blood glucose concentration after food intake. GLUT4 operates largely independently but may interact with other glucose transporters like GLUT1 to maintain normal cellular glucose homeostasis.

Pathways

GLUT4 plays a significant role in the insulin signaling pathway and glucose homeostasis. Activation of the insulin receptor triggers a signaling cascade that includes enzymes like Akt (protein kinase B) which facilitates the translocation of GLUT4 vesicles to the plasma membrane. This translocation is important for glucose entry into the cells. Other key proteins in this pathway include IRS (insulin receptor substrate) proteins which act as signaling intermediates to link insulin receptor activation to downstream targets like GLUT4. Its activity is central to the maintenance of energy balance and storage in the body.

GLUT4 is closely associated with conditions like type 2 diabetes and obesity. Impaired GLUT4 function or expression can lead to decreased insulin sensitivity and glucose uptake contributing to the hyperglycemic state found in type 2 diabetes. Additionally obesity often presents a parallel decline in GLUT4 activity exacerbating insulin resistance. The regulation of GLUT4 is influenced by proteins such as insulin receptor and IRS proteins which when dysfunctional contribute to the pathogenesis of these metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed : 26240143, PubMed : 26629404). Response to insulin is regulated by its intracellular localization : in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed : 26240143, PubMed : 26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed : 26240143, PubMed : 26629404).

See full target information Slc2a4

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed39858418

2025

Extracellular Vesicles and Tunnelling Nanotubes as Mediators of Prostate Cancer Intercellular Communication.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica K Heatlie,Joanna Lazniewska,Courtney R Moore,Ian R D Johnson,Bukuru D Nturubika,Ruth Williams,Mark P Ward,John J O'Leary,Lisa M Butler,Doug A Brooks

Annals of translational medicine 11:247 PubMed37082665

2023

Diane-35 and metformin therapy in rats with endometrial lesions induced by dihydrotestosterone exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Liu,Ran Xu,Yifan Zhou,Yang Wang,Feifei Zhang,Xiaoyu Tong,Peng Cui,Tong Ma,Jian Sun,Yi Feng,Xin Li

Journal of molecular and cellular cardiology 164:136-147 PubMed34923199

2021

A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Emanuelli,Anna Zoccarato,Christina M Reumiller,Angelos Papadopoulos,Mei Chong,Sabine Rebs,Kai Betteridge,Matteo Beretta,Katrin Streckfuss-Bömeke,Ajay M Shah

PloS one 16:e0252707 PubMed34133443

2021

Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Ece Derici,Sibel Özdaş,İpek Canatar,Murat Koç

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21466 PubMed33734496

2021

Mixed conjugated linoleic acid sex-dependently reverses high-fat diet-induced insulin resistance via the gut-adipose axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyu Li,Pan Zhuang,Yu Zhang,Qiyang Shou,Yanhua Lu,Guangfa Wang,Jieni Qiu,Jun Wang,Lilin He,Jingnan Chen,Jingjing Jiao

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21295 PubMed33475194

2021

TMF1 is upregulated by insulin and is required for a sustained glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Roni Rahimi,Israel Malek,Tali Lerrer-Goldshtein,Yoav Elkis,Irit Shoval,Avi Jacob,Sally Shpungin,Uri Nir

Cells 9: PubMed32041272

2020

Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kippeum Lee,Heegu Jin,Sungwoo Chei,Hyun-Ji Oh,Jeong-Yong Lee,Boo-Yong Lee

Brain structure & function 224:315-336 PubMed30317390

2018

Modulation of olfactory-driven behavior by metabolic signals: role of the piriform cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Dolly Al Koborssy,Brigitte Palouzier-Paulignan,Vincent Canova,Marc Thevenet,Debra Ann Fadool,Andrée Karyn Julliard

Scientific reports 7:4580 PubMed28676641

2017

Dynamin-2 mutations linked to Centronuclear Myopathy impair actin-dependent trafficking in muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arlek M González-Jamett,Ximena Baez-Matus,María José Olivares,Fernando Hinostroza,Maria José Guerra-Fernández,Jacqueline Vasquez-Navarrete,Mai Thao Bui,Pascale Guicheney,Norma Beatriz Romero,Jorge A Bevilacqua,Marc Bitoun,Pablo Caviedes,Ana M Cárdenas

FASEB journal : official publication of the Federa 31:3138-3149 PubMed28386046

2017

Pharmacological Sirt6 inhibition improves glucose tolerance in a type 2 diabetes mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Sociali,Mirko Magnone,Silvia Ravera,Patrizia Damonte,Tiziana Vigliarolo,Maria Von Holtey,Valerio G Vellone,Enrico Millo,Irene Caffa,Michele Cea,Marco Daniele Parenti,Alberto Del Rio,Maximilien Murone,Raul Mostoslavsky,Alessia Grozio,Alessio Nencioni,Santina Bruzzone

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Glucose Transporter GLUT4 antibody [1F8]

Western blot - Anti-Glucose Transporter GLUT4 antibody [1F8] (AB35826)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise