AB216661

Anti-Glucose Transporter GLUT4 antibody

Name: Anti-Glucose Transporter GLUT4 antibody (ab216661) | Abcam
Brand: Abcam Limited
SKU: AB216661
Price: 535 USD
Availability: InStock

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(12 Publications)

Rabbit Polyclonal Glucose Transporter GLUT4 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human SLC2A4 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GLUT4, SLC2A4, GLUT-4

3 Images

Supplier Data

Western blot - Anti-Glucose Transporter GLUT4 antibody (AB216661)

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 antibody (ab216661) at 1/300 dilution

All lanes:

mouse heart lysates

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP conjugated (90min 37˚C) at 1/5000 dilution

Predicted band size: 54 kDa

false

Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT4 antibody (AB216661)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT4 antibody (AB216661)

Intracellular Flow Cytometry of K562 cells with ab203387 at 1/100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (AB216661)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (AB216661)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling Glucose Transporter GLUT4 with ab216661 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human SLC2A4 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P14672

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose transporter GLUT4 also known as GLUT-4 or GLUT 4 transporter plays an important role in glucose uptake in cells. It is an insulin-responsive class I facilitated glucose transporter. GLUT4 predominantly expresses in insulin-sensitive tissues such as adipose tissue and skeletal and cardiac muscles. The functional transport mechanism facilitates glucose entry into the cell by translocating from intracellular vesicles to the plasma membrane in response to insulin stimulation. The molecular weight of GLUT4 is about 50 kDa and this property is often observed in techniques such as GLUT4 western blot where it aids in the identification of the transporter.

Biological function summary

GLUT4 helps regulate blood sugar levels through its control over glucose uptake in muscle and adipose tissues. As an important component of the insulin-regulated glucose transport system GLUT4 responds to increased insulin levels by moving to the cell's surface aiding in glucose transportation into the cell. This adaptive response reduces blood glucose concentration after food intake. GLUT4 operates largely independently but may interact with other glucose transporters like GLUT1 to maintain normal cellular glucose homeostasis.

Pathways

GLUT4 plays a significant role in the insulin signaling pathway and glucose homeostasis. Activation of the insulin receptor triggers a signaling cascade that includes enzymes like Akt (protein kinase B) which facilitates the translocation of GLUT4 vesicles to the plasma membrane. This translocation is important for glucose entry into the cells. Other key proteins in this pathway include IRS (insulin receptor substrate) proteins which act as signaling intermediates to link insulin receptor activation to downstream targets like GLUT4. Its activity is central to the maintenance of energy balance and storage in the body.

GLUT4 is closely associated with conditions like type 2 diabetes and obesity. Impaired GLUT4 function or expression can lead to decreased insulin sensitivity and glucose uptake contributing to the hyperglycemic state found in type 2 diabetes. Additionally obesity often presents a parallel decline in GLUT4 activity exacerbating insulin resistance. The regulation of GLUT4 is influenced by proteins such as insulin receptor and IRS proteins which when dysfunctional contribute to the pathogenesis of these metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization : in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.

See full target information SLC2A4

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 23:94 PubMed38566151

2024

GLUT4 localisation with the plasma membrane is unaffected by an increase in plasma free fatty acid availability.

Applications

Unspecified application

Species

Unspecified reactive species

J S Barrett,J A Strauss,L S Chow,S O Shepherd,A J M Wagenmakers,Y Wang

International journal of molecular sciences 25: PubMed38396865

2024

Sex-Specific Effects of Long-Term Antipsychotic Drug Treatment on Adipocyte Tissue and the Crosstalk to Liver and Brain in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Fehsel,Marie-Luise Bouvier

International journal of molecular sciences 24: PubMed38003735

2023

Increased IGFBP2 Levels by Placenta-Derived Mesenchymal Stem Cells Enhance Glucose Metabolism in a TAA-Injured Rat Model via AMPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dae-Hyun Lee,Hyeri Park,Jun-Hyeong You,Jin Seok,Dong-Wook Kwon,Young-Ran Kim,Gi-Jin Kim

Molecular medicine reports 26: PubMed35552758

2022

Role of metformin in functional endometrial hyperplasia and polycystic ovary syndrome involves the regulation of MEG3/miR‑223/GLUT4 and SNHG20/miR‑4486/GLUT4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yangchun Zhao,Long Chen,Ruilan Li,Yumei Ning,Xiuzhi Zhu

Phytotherapy research : PTR 36:1297-1309 PubMed35088915

2022

A novel DPP-4 inhibitor Gramcyclin A attenuates cognitive deficits in APP/PS1/tau triple transgenic mice via enhancing brain GLP-1-dependent glucose uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Zongyang Li,Yuan Zhang,Xiangbao Meng,Min Li,Weiwei Cao,Junshan Yang,Xudong Xu,Wenlan Liu,Weiping Li,Qian Cai,Sicen Wang,Guoxu Ma,Zhiheng Liu,Guodong Huang

Oxidative medicine and cellular longevity 2022:3182931 PubMed35126811

2022

miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghong Zhu,Xuewen Yang,Jing Zhou,Long Chen,Pengfei Zuo,Lin Chen,Lan Jiang,Ting Li,Dejiang Wang,Yingyi Xu,Qiushi Li,Yi Yan

Adipocyte 11:84-98 PubMed35067163

2022

The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Qian Wu,Yuan Zhou,Liangyu Yu,Lixiu Yu,Yahui Deng,Chuyue Tu,Weiyong Li

Experimental and therapeutic medicine 23:78 PubMed34938364

2021

Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Li,Huan Gu

Biomedicines 9: PubMed34680568

2021

Chip-Based Sensing of the Intercellular Transfer of Cell Surface Proteins: Regulation by the Metabolic State.

Applications

Unspecified application

Species

Unspecified reactive species

Günter A Müller,Matthias H Tschöp,Timo D Müller

Cell death discovery 7:154 PubMed34226523

2021

Methyltransferase-like protein 7A (METTL7A) promotes cell survival and osteogenic differentiation under metabolic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Eugene Lee,Ju-Young Kim,Tae-Kyung Kim,Seo-Young Park,Gun-Il Im

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT4 antibody

Western blot - Anti-Glucose Transporter GLUT4 antibody (AB216661)

Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT4 antibody (AB216661)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (AB216661)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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