Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)

Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) is a rabbit monoclonal antibody detecting Glucose Transporter GLUT4 in Western Blot, IP, IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse, Rat.

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Images

Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (AB313775), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB	IP	Dot	Flow Cyt (Intra)
Human	Tested	Expected	Tested	Expected	Expected	Not recommended
Mouse	Tested	Tested	Tested	Tested	Expected	Not recommended
Rat	Tested	Expected	Tested	Expected	Expected	Not recommended
Transfected cell lysate - Human	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information Slc2a4

Function

Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed:26240143, PubMed:26629404). Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed:26240143, PubMed:26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed:26240143, PubMed:26629404).

Alternative names

Glut4, Slc2a4, GT2, GLUT-4

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26571-45
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of Glucose Transporter GLUT4?

Anti-Glucose Transporter GLUT4 [EPR26571-45] (ab313775) specifically detects a band for Glucose Transporter GLUT4 (UniProt: P14142) at a molecular weight of 54kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR26571-45] also available for your convenience:
ab313775, Carrier free - ab313776

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glucose transporter GLUT4 also known as GLUT-4 or GLUT 4 transporter plays an important role in glucose uptake in cells. It is an insulin-responsive class I facilitated glucose transporter. GLUT4 predominantly expresses in insulin-sensitive tissues such as adipose tissue and skeletal and cardiac muscles. The functional transport mechanism facilitates glucose entry into the cell by translocating from intracellular vesicles to the plasma membrane in response to insulin stimulation. The molecular weight of GLUT4 is about 50 kDa and this property is often observed in techniques such as GLUT4 western blot where it aids in the identification of the transporter.

Biological function summary

GLUT4 helps regulate blood sugar levels through its control over glucose uptake in muscle and adipose tissues. As an important component of the insulin-regulated glucose transport system GLUT4 responds to increased insulin levels by moving to the cell's surface aiding in glucose transportation into the cell. This adaptive response reduces blood glucose concentration after food intake. GLUT4 operates largely independently but may interact with other glucose transporters like GLUT1 to maintain normal cellular glucose homeostasis.

Pathways

GLUT4 plays a significant role in the insulin signaling pathway and glucose homeostasis. Activation of the insulin receptor triggers a signaling cascade that includes enzymes like Akt (protein kinase B) which facilitates the translocation of GLUT4 vesicles to the plasma membrane. This translocation is important for glucose entry into the cells. Other key proteins in this pathway include IRS (insulin receptor substrate) proteins which act as signaling intermediates to link insulin receptor activation to downstream targets like GLUT4. Its activity is central to the maintenance of energy balance and storage in the body.

Associated diseases and disorders

GLUT4 is closely associated with conditions like type 2 diabetes and obesity. Impaired GLUT4 function or expression can lead to decreased insulin sensitivity and glucose uptake contributing to the hyperglycemic state found in type 2 diabetes. Additionally obesity often presents a parallel decline in GLUT4 activity exacerbating insulin resistance. The regulation of GLUT4 is influenced by proteins such as insulin receptor and IRS proteins which when dysfunctional contribute to the pathogenesis of these metabolic disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Glucose Transporter GLUT4 Western blot staining using rabbit Anti-Glucose Transporter GLUT4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver (PMID: 2654938).

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
Exposure time: 48 seconds
All lanes: Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Observed band size: 51 kDa
Exposure time: 48s
Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Glucose Transporter GLUT4 Western blot staining using rabbit Anti-Glucose Transporter GLUT4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver (PMID: 2654938), lung (PMID: 2654938).

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) at 1/1000 dilution
Lane 1: Rat heart tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 20 µg
Lane 3: Rat lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa
Exposure time: 15s
Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) at 1/1000 dilution
Lane 1: RD (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg
Lane 2: A673 (human muscle Ewing's Sarcoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 51 kDa
Exposure time: 180s
Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) at 1/1000 dilution
Lane 1: Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: 3T3-L1 differentiation for 6 days whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 51 kDa
Exposure time: 158s
Immunoprecipitation - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
SLC2A4 was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab313775 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313775 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse heart tissue lysate 5 µg Lane 2: abab313775 IP in Mouse heart tissue lysate Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313775 in mouse heart tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27302065).
All lanes: Immunoprecipitation - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) at 1/30 dilution
All lanes: Mouse heart tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: kidney (PMID: 2654938).

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 105 seconds
All lanes: Western blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa
Exposure time: 105s
Dot Blot - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Dot blot analysis of SLC2A4 using ab313775 at 1:1000 (0.518 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution. Exposure time: 8 seconds. Blocking and diluting buffer and concentration: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling SLC2A4 with ab313775 at 1/100 (5.18 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in differentiated 3T3-L1 cells, and no staining in 3T3-L1 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8) Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat liver. The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse liver. The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human liver. The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cardiac muscle. The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse skeletal muscle (PMID: 2654938; PMID: 23877319; PMID: 33061888). The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody [EPR26571-45] (ab313775)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SLC2A4 with ab313775 at 1/5000 (0.104 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab313775 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins