AB48547

Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]

Name: Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) | Abcam
Brand: Abcam Limited
SKU: AB48547
Price: 475 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(13 Publications)

Mouse Monoclonal Glucose Transporter GLUT4 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 13 publications.

View Alternative Names

GLUT4, SLC2A4, GLUT-4

1 Images

Project

Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (AB48547)

ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/mL

All lanes:

Partial tagged recombinant protein to GLUT4 at 0.1 µg

Secondary

All lanes:

Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/3000 dilution

Predicted band size: 54 kDa

Observed band size: 30 kDa

false

Carrier free

Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam48547

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Sheep": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose transporter GLUT4 also known as GLUT-4 or GLUT 4 transporter plays an important role in glucose uptake in cells. It is an insulin-responsive class I facilitated glucose transporter. GLUT4 predominantly expresses in insulin-sensitive tissues such as adipose tissue and skeletal and cardiac muscles. The functional transport mechanism facilitates glucose entry into the cell by translocating from intracellular vesicles to the plasma membrane in response to insulin stimulation. The molecular weight of GLUT4 is about 50 kDa and this property is often observed in techniques such as GLUT4 western blot where it aids in the identification of the transporter.

Biological function summary

GLUT4 helps regulate blood sugar levels through its control over glucose uptake in muscle and adipose tissues. As an important component of the insulin-regulated glucose transport system GLUT4 responds to increased insulin levels by moving to the cell's surface aiding in glucose transportation into the cell. This adaptive response reduces blood glucose concentration after food intake. GLUT4 operates largely independently but may interact with other glucose transporters like GLUT1 to maintain normal cellular glucose homeostasis.

Pathways

GLUT4 plays a significant role in the insulin signaling pathway and glucose homeostasis. Activation of the insulin receptor triggers a signaling cascade that includes enzymes like Akt (protein kinase B) which facilitates the translocation of GLUT4 vesicles to the plasma membrane. This translocation is important for glucose entry into the cells. Other key proteins in this pathway include IRS (insulin receptor substrate) proteins which act as signaling intermediates to link insulin receptor activation to downstream targets like GLUT4. Its activity is central to the maintenance of energy balance and storage in the body.

GLUT4 is closely associated with conditions like type 2 diabetes and obesity. Impaired GLUT4 function or expression can lead to decreased insulin sensitivity and glucose uptake contributing to the hyperglycemic state found in type 2 diabetes. Additionally obesity often presents a parallel decline in GLUT4 activity exacerbating insulin resistance. The regulation of GLUT4 is influenced by proteins such as insulin receptor and IRS proteins which when dysfunctional contribute to the pathogenesis of these metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization : in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.

See full target information SLC2A4

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Adipocyte 13:2374062 PubMed38953241

2024

Silybin restores glucose uptake after tumour necrosis factor-alpha and lipopolysaccharide stimulation in 3T3-L1 adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Butanda-Nuñez,Octavio Rodríguez-Cortés,Espiridión Ramos-Martínez,Marco Antonio Cerbón,Galileo Escobedo,Anahí Chavarría

Environmental toxicology 39:1269-1282 PubMed37927237

2023

SNHG1, interacting with SND1, contributes to sorafenib resistance of liver cancer cells by increasing m6A-mediated SLC7A11 expression and promoting aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Shunle Li,Lin Shen,Junhui Li,Di Zhang,Jinmin Yu,Lanxuan Huang,Na Liu,Hongwei Lu,Meng Xu

International journal of molecular sciences 24: PubMed37834351

2023

Preventive Effect of Molecular Iodine in Pancreatic Disorders from Hypothyroid Rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Rodríguez-Castelán,Evangelina Delgado-González,Esteban Rodríguez-Benítez,Francisco Castelán,Estela Cuevas-Romero,Brenda Anguiano,Michael C Jeziorski,Carmen Aceves

Journal of biochemical and molecular toxicology 38:e23515 PubMed37632267

2023

Advanced glycation end products induce nucleus pulposus cell apoptosis by upregulating TXNIP via inhibiting glycolysis pathway in intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Chen,Xiaoping Sheng,Haobo Sun,Qunfeng Guo,Haibin Wang,Lecheng Wu,Bin Ni,Jun Yang

Antioxidants (Basel, Switzerland) 12: PubMed37237932

2023

The Impact of Resveratrol-Enriched Bread on Cardiac Remodeling in a Preclinical Model of Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia F R Silva,Rita Silva-Reis,Rita Ferreira,Paula A Oliveira,Ana I Faustino-Rocha,Maria de Lurdes Pinto,Manuel A Coimbra,Artur M S Silva,Susana M Cardoso

Clinical and translational medicine 12:e699 PubMed35184403

2022

Persistent high glucose induced EPB41L4A-AS1 inhibits glucose uptake via GCN5 mediating crotonylation and acetylation of histones and non-histones.

Applications

Unspecified application

Species

Unspecified reactive species

Weijie Liao,Naihan Xu,Haowei Zhang,Weifang Liao,Yanzhi Wang,Songmao Wang,Shikuan Zhang,Yuyang Jiang,Weidong Xie,Yaou Zhang

Frontiers in genetics 11:1022 PubMed33101365

2020

Insulin-Independent and Dependent Glucose Transporters in Brain Mural Cells in CADASIL.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmod Panahi,Patricia Rodriguez Rodriguez,Seyed-Mohammad Fereshtehnejad,Donia Arafa,Nenad Bogdanovic,Bengt Winblad,Angel Cedazo-Minguez,Juha Rinne,Taher Darreh-Shori,Yoshiki Hase,Raj N Kalaria,Matti Viitanen,Homira Behbahani

PloS one 13:e0195960 PubMed29677207

2018

Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Emi Ono,Hiroyuki Murota,Yuki Mori,Yoshichika Yoshioka,Yuko Nomura,Takichi Munetsugu,Hiroo Yokozeki,Ichiro Katayama

Biochimica et biophysica acta. Molecular basis of 1863:2924-2933 PubMed28844948

2017

Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jibiao Li,Cheng Chen,Yuan Li,David J Matye,Yifeng Wang,Wen-Xing Ding,Tiangang Li

Diabetes, obesity & metabolism 19:1135-1146 PubMed28244693

2017

Dapagliflozin improves insulin resistance and glucose intolerance in a novel transgenic rat model of chronic glucose overproduction and glucose toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Christos N Joannides,Salvatore P Mangiafico,Matthew F Waters,Benjamin J Lamont,Sofianos Andrikopoulos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]

Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (AB48547)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise