AB188317

Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

Name: Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) | Abcam
Brand: Abcam Limited
SKU: AB188317
Price: 365 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(3 Reviews)

(13 Publications)

Rabbit Recombinant Monoclonal Glucose Transporter GLUT4 phospho S488 + S488 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 13 publications.

View Alternative Names

GLUT4, SLC2A4, GLUT-4

5 Images

Lab

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

Lane 1:

Human fetal heart whole cell lysate at 15 µg

Lane 2:

Human fetal heart whole cell lysate + Phosphatase at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa

false

Exposure time: 1s

Lab

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Lanes 1 and 2 : 7 seconds exposure

Lanes 3 and 4 : 20 seconds exposure

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

Lane 1:

Human heart lysate, boiled at 20 µg

Lane 2:

Human heart lysate, unboiled at 20 µg

Lane 3:

Mouse heart lysate, boiled at 20 µg

Lane 4:

Mouse heart lysate, unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa

false

Supplier Data

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution

Lane 1:

Untreated human fetal heart at 10 µg

Lane 2:

Lambda phosphorylase treated human fetal heart at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa

false

Supplier Data

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

All lanes:

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa

false

Lab

Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Dot Blot analysis of Lane 1 : Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2 : Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution.

5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time : 3 minutes.

Carrier free

Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR930(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "We recommend not to boil the samples before loading to get desirable WB results." }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "We recommend not to boil the samples before loading to get desirable WB results." }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "We recommend not to boil the samples before loading to get desirable WB results." }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose transporter GLUT4 also known as GLUT-4 or GLUT 4 transporter plays an important role in glucose uptake in cells. It is an insulin-responsive class I facilitated glucose transporter. GLUT4 predominantly expresses in insulin-sensitive tissues such as adipose tissue and skeletal and cardiac muscles. The functional transport mechanism facilitates glucose entry into the cell by translocating from intracellular vesicles to the plasma membrane in response to insulin stimulation. The molecular weight of GLUT4 is about 50 kDa and this property is often observed in techniques such as GLUT4 western blot where it aids in the identification of the transporter.

Biological function summary

GLUT4 helps regulate blood sugar levels through its control over glucose uptake in muscle and adipose tissues. As an important component of the insulin-regulated glucose transport system GLUT4 responds to increased insulin levels by moving to the cell's surface aiding in glucose transportation into the cell. This adaptive response reduces blood glucose concentration after food intake. GLUT4 operates largely independently but may interact with other glucose transporters like GLUT1 to maintain normal cellular glucose homeostasis.

Pathways

GLUT4 plays a significant role in the insulin signaling pathway and glucose homeostasis. Activation of the insulin receptor triggers a signaling cascade that includes enzymes like Akt (protein kinase B) which facilitates the translocation of GLUT4 vesicles to the plasma membrane. This translocation is important for glucose entry into the cells. Other key proteins in this pathway include IRS (insulin receptor substrate) proteins which act as signaling intermediates to link insulin receptor activation to downstream targets like GLUT4. Its activity is central to the maintenance of energy balance and storage in the body.

GLUT4 is closely associated with conditions like type 2 diabetes and obesity. Impaired GLUT4 function or expression can lead to decreased insulin sensitivity and glucose uptake contributing to the hyperglycemic state found in type 2 diabetes. Additionally obesity often presents a parallel decline in GLUT4 activity exacerbating insulin resistance. The regulation of GLUT4 is influenced by proteins such as insulin receptor and IRS proteins which when dysfunctional contribute to the pathogenesis of these metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization : in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.

See full target information SLC2A4 phospho S488 + S488

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:374 PubMed41044623

2025

THBS1 regulates the function and insulin sensitivity of HTR8/SVneo cells treated with high glucose through the RhoA/ROCK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuerong Chai,Jie Tang,Aimin Liu,Qin Zhou

Cell communication and signaling : CCS 22:503 PubMed39420342

2024

Acetylcysteine synergizes PD-1 blockers against colorectal cancer progression by promoting TCF1PD1CD8 T cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchang Zhou,Mengdi Qu,Ying Yue,Ziwen Zhong,Ke Nan,Xingfeng Sun,Qichao Wu,Jie Zhang,Wankun Chen,Changhong Miao

Journal of vascular research 61:179-196 PubMed38952123

2024

Nitric Oxide/Glucose Transporter Type 4 Pathway Mediates Cardioprotection against Ischemia/Reperfusion Injury under Hyperglycemic and Diabetic Conditions in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Aisha Al-Kouh,Fawzi Babiker

Aging and disease 14:529-547 PubMed37008055

2023

Positive Feedback Regulation of Circular RNA Hsa_circ_0000566 and HIF-1α promotes Osteosarcoma Progression and Glycolysis Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shuying Shen,Yining Xu,Zhe Gong,Teng Yao,Di Qiao,Yizhen Huang,Zhenlei Zhang,Jun Gao,Haonan Ni,Zhanping Jin,Yingchun Zhu,Hongfei Wu,Qingxin Wang,Xiangqian Fang,Kangmao Huang,Jianjun Ma

Pharmaceuticals (Basel, Switzerland) 16: PubMed37259385

2023

Renin-Angiotensin System Antagonism Protects the Diabetic Heart from Ischemia/Reperfusion Injury in Variable Hyperglycemia Duration Settings by a Glucose Transporter Type 4-Mediated Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Aisha Al-Kouh,Fawzi Babiker,Maie Al-Bader

International journal of oncology 62: PubMed36416344

2022

Overcharged lipid metabolism in mechanisms of antitumor by ‑derived polysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Xiumin Li,Qiaoling Su,Yutian Pan

Molecular medicine reports 26: PubMed35552758

2022

Role of metformin in functional endometrial hyperplasia and polycystic ovary syndrome involves the regulation of MEG3/miR‑223/GLUT4 and SNHG20/miR‑4486/GLUT4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yangchun Zhao,Long Chen,Ruilan Li,Yumei Ning,Xiuzhi Zhu

Cell communication and signaling : CCS 20:40 PubMed35346238

2022

An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Guo-Jun Geng,Jian-Guang Guo,Yan-Jun Mi,Xiao-Lei Zhu,Ning Li,Hong-Ming Liu,Jun-Feng Lin,Jian-Weng Wang,Guang Zhao,Guan-Zhi Ye,Bo-An Li,Qi-Cong Luo,Jie Jiang

Frontiers in endocrinology 12:660815 PubMed33859622

2021

D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Magdalena Montt-Guevara,Michele Finiguerra,Ilaria Marzi,Tiziana Fidecicchi,Amerigo Ferrari,Alessandro D Genazzani,Tommaso Simoncini

Nutrition & metabolism 18:14 PubMed33468193

2021

A combination of herbal compound (SPTC) along with exercise or metformin more efficiently alleviated diabetic complications through down-regulation of stress oxidative pathway upon activating Nrf2-Keap1 axis in AGE rich diet-induced type 2 diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Golbarg Rahimi,Salime Heydari,Bahareh Rahimi,Navid Abedpoor,Iman Niktab,Zahra Safaeinejad,Maryam Peymani,Farzad Seyed Forootan,Zahra Derakhshan,Mohammad Hossein Nasr Esfahani,Kamran Ghaedi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise