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Rabbit Recombinant Monoclonal Glucosidase 2 subunit beta antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (AB129098), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (AB129098), expandable thumbnail
  • Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (AB129098), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (AB129098), expandable thumbnail
  • OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (AB129098), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:10929008). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).

Alternative names

G19P1, PRKCSH, Glucosidase 2 subunit beta, 80K-H protein, Glucosidase II subunit beta, Protein kinase C substrate 60.1 kDa protein heavy chain, PKCSH

Recommended products

Rabbit Recombinant Monoclonal Glucosidase 2 subunit beta antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8047
Purification technique
Affinity purification Protein A
Dissociation constant
1.1 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glucosidase 2 subunit beta also known as PRKCSH plays an important role in protein processing within the endoplasmic reticulum (ER). This protein forms part of the heterodimeric enzyme complex glucosidase II responsible for cleaving glucose molecules during glycoprotein maturation. With a molecular weight of about 60 kDa glucosidase 2 subunit beta is important for efficient N-linked glycosylation which is a fundamental process for protein folding and quality control. This protein localizes mainly to the ER where it contributes to proper cellular functions by aiding in the preparation of proteins for trafficking to their final destinations.

Biological function summary

Glucosidase 2 subunit beta is associated with protein maturation and quality control within the ER lumen. It is part of the glucosidase II complex which functions to trim glucose residues from N-linked oligosaccharides on nascent glycoproteins facilitating their proper folding and preventing misfolded proteins' accumulation. This activity ensures that only properly folded proteins progress through the secretory pathway while faulty proteins are targeted for degradation. Through these processes glucosidase 2 subunit beta supports cellular homeostasis and protein turnover.

Pathways

Glucosidase 2 subunit beta plays an influential role in the protein processing and ER-associated degradation (ERAD) pathways. The protein operates within these pathways to regulate glycoprotein folding impacting the unfolding protein response (UPR). It interacts with several proteins including calnexin and calreticulin which are key players in the glycoprotein folding process. Moreover the proper function of glucosidase 2 subunit beta in these pathways is necessary for maintaining the cellular stress response ultimately ensuring efficient cell function and survival.

Associated diseases and disorders

Defects in glucosidase 2 subunit beta have links to autosomal dominant polycystic liver disease (PCLD). Mutations in PRKCSH which encodes glucosidase 2 subunit beta can lead to the development of PCLD due to disrupted glycoprotein folding and ER stress-induced apoptosis. Additionally abnormalities in its function may indirectly connect to diabetes mellitus by affecting insulin receptor glycosylation and secretion processes. These relationships illustrate the broader impact of glucosidase 2 subunit beta on health and disease providing insight into potential therapeutic targets for treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098), expandable thumbnail

    Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)

    Lanes 1- 4: Merged signal (red and green). Green - ab129098 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab129098 was shown to react with PRKCSH in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human PRKCSH (Glucosidase 2 subunit beta) knockout HEK-293T cell line ab266770 (knockout cell lysate Human PRKCSH (Glucosidase 2 subunit beta) knockout HEK-293T cell lysate ab257608) was used. Wild-type HEK-293T and PRKCSH knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129098 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: PRKCSH knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human PRKCSH (Glucosidase 2 subunit beta) knockout HEK-293T cell line (Human PRKCSH (Glucosidase 2 subunit beta) knockout HEK-293T cell line ab266770)

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: Daudi cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 59 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)

    ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098), expandable thumbnail

    Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)

    All lanes: Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution

    Lane 1: HeLa cell lysates at 10 µg

    Lane 2: Jurkat cell lysates at 10 µg

    Lane 3: A431 cell lysates at 10 µg

    Lane 4: K562 cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)

    ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in HeLa cells by Immunofluorescence.

  • OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098), expandable thumbnail

    OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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