Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free

• WB

Lab

Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (AB248297)

This data was developed using the same antibody clone in a different buffer formulation (ab129098).

Lanes 1- 4 : Merged signal (red and green). Green - ab129098 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab129098 was shown to react with PRKCSH in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266770 (knockout cell lysate ab257608) was used. Wild-type HEK-293T and PRKCSH knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129098 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (<a href='/en-us/products/primary-antibodies/glucosidase-2-subunit-beta-antibody-epr8047-ab129098'>ab129098</a>) at 1/10000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PRKCSH knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRKCSH (Glucosidase 2 subunit beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-prkcsh-glucosidase-2-subunit-beta-knockout-hek-293t-cell-line-ab266770'>ab266770</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 59 kDa

Observed band size: 80 kDa

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• WB

Unknown

Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (AB248297)

This data was developed using ab129098, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

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• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (AB248297)

This data was developed using ab129098, the same antibody clone in a different buffer formulation.ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in HeLa cells by Immunofluorescence.

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (AB248297)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.