AB153973

Anti-GLUD1 antibody

Name: Anti-GLUD1 antibody (ab153973) | Abcam
Brand: Abcam Limited
SKU: AB153973
Price: 545 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal GLUD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Drosophila melanogaster samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLUD1 aa 50-400.

View Alternative Names

GLUD, GLUD1, GDH 1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody (AB153973)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody (AB153973)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GLUD1 with ab153973 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 antibody (AB153973)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 antibody (AB153973)

Immunofluorescence analysis of U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min labelling Glutamate Dehydrogenase protein at cytoplasm using ab153973 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 antibody (AB153973)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling Glutamate Dehydrogenase protein at mitochondria using ab153973 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody (AB153973)

Immunohistochemical analysis of paraffin-embedded rat liver tissue stained for Glutamate Dehydrogenase protein at mitochondria with ab153973 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-GLUD1 antibody (AB153973)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-GLUD1 antibody (ab153973) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa; whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-GLUD1 antibody (AB153973)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-GLUD1 antibody (ab153973) at 1/1000 dilution

All lanes:

Drosophila tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GLUD1 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

P00367

Specificity

Due to high sequence homology with GLUD2 there is a potential for cross-reactivity.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD1 also known as glutamate dehydrogenase 1 is an enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and ammonia playing an important role in amino acid metabolism. The molecular mass of GLUD1 is approximately 61 kDa. It is expressed in many tissues with notably high levels in liver kidney and neural tissues. GLUD1 is essential for maintaining the balance between amino acid and carbohydrate metabolism.

Biological function summary

GLUD1 facilitates nitrogen and carbon homeostasis through its role in amino acid breakdown and energy metabolism. Although not part of a complex GLUD1's activity impacts central metabolic processes influencing cellular energy status and neurotransmitter regulation. The enzyme contributes to the glutamate-glutamine cycle which is important for neurotransmission in the brain. Its regulation by ADP GDP and other molecules enables cells to adapt to energetic demands.

Pathways

GLUD1 is integral to the tricarboxylic acid (TCA) cycle and urea cycle impacting both energy production and detoxification of ammonia. In the TCA cycle GLUD1 influences the availability of α-ketoglutarate interfacing with enzymes such as isocitrate dehydrogenase. In the urea cycle it aids in converting ammonia to urea in the liver. Additionally GLUD1's function connects with glutamine synthetase in managing amino acid synthesis and degradation.

GLUD1 is associated with congenital hyperinsulinism and hepatic encephalopathy. Hyperinsulinism often results from mutations in GLUD1 which lead to excessive insulin release due to increased leucine sensitivity. In hepatic encephalopathy GLUD1's role in ammonia detoxification becomes important with impaired function potentially leading to elevated ammonia levels and neurological dysfunction. Understanding GLUD1's interactions with other proteins such as those in insulin signaling pathways could further elucidate its impact on these medical conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

See full target information GLUD1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:542 PubMed40442713

2025

A-485 alleviates postmenopausal osteoporosis by activating GLUD1 deacetylation through the SENP1-Sirt3 signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghong Ma,Xiaohua Zou,Qianhong Jian,Jiaxin Dong,Xianbing Huang,Yue Zhai,Li Qian

Frontiers in cell and developmental biology 9:719187 PubMed34765600

2021

Transcriptional Repression of Ferritin Light Chain Increases Ferroptosis Sensitivity in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Wang,Shiyu Qiu,Hong Wang,Jiangtao Cui,Xiaoting Tian,Yayou Miao,Congcong Zhang,Leiqun Cao,Lifang Ma,Xin Xu,Yongxia Qiao,Xiao Zhang

The Journal of cell biology 220: PubMed34623384

2021

PINK1 drives production of mtDNA-containing extracellular vesicles to promote invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Rabas,Sarah Palmer,Louise Mitchell,Shehab Ismail,Andrea Gohlke,Joel S Riley,Stephen W G Tait,Payam Gammage,Leandro Lemgruber Soares,Iain R Macpherson,Jim C Norman

Veterinary and comparative oncology 16:239-245 PubMed29266697

2017

Expression of the glutamine metabolism-related proteins glutaminase 1 and glutamate dehydrogenase in canine mammary tumours.

Applications

Unspecified application

Species

Unspecified reactive species

J-E Ryu,H-K Park,H-J Choi,H-B Lee,H-J Lee,H Lee,E-S Yu,W-C Son

Endocrine-related cancer 20:339-48 PubMed23507704

2013

Expression of glutamine metabolism-related proteins according to molecular subtype of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sewha Kim,Do Hee Kim,Woo-Hee Jung,Ja Seung Koo

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GLUD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody (AB153973)

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 antibody (AB153973)

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 antibody (AB153973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody (AB153973)

Western blot - Anti-GLUD1 antibody (AB153973)

Western blot - Anti-GLUD1 antibody (AB153973)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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