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Rabbit Polyclonal GLUD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Glutamate dehydrogenase 2, mitochondrial aa 250 to C-terminus.

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Images

Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027), expandable thumbnail
  • Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (AB154027), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody (AB154027), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Glutamate dehydrogenase 2, mitochondrial aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49448

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed:11032875, PubMed:11254391, PubMed:16023112, PubMed:16959573). Plays a role in insulin homeostasis (PubMed:11297618, PubMed:9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

Targets

Glutamate dehydrogenase 2, mitochondrial

Alternative names

Recommended products

Rabbit Polyclonal GLUD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Glutamate dehydrogenase 2, mitochondrial aa 250 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Glutamate dehydrogenase 2, mitochondrial aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49448
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GLUD1 and GLUD2 also known as glutamate dehydrogenase 1 and 2 are enzymes that catalyze the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. The mass of GLUD1 is approximately 61 kDa and it is expressed in multiple tissues with high levels in the liver brain and pancreas. GLUD2 is expressed mainly in the brain. These enzymes are integral for regulating amino acid metabolism and are activated by ADP and inhibited by GTP.

Biological function summary

GLUD1 and GLUD2 influence cellular energy homeostasis by modulating levels of glutamate and alpha-ketoglutarate. They function as homodimers or heterodimers and play a role in neurotransmitter cycling in neural tissues. This involvement in neurotransmitter metabolism is significant as they ensure glutamate levels remain balanced preventing excitotoxicity.

Pathways

GLUD1 and GLUD2 participate in the urea cycle and glutamate metabolism. They work alongside other enzymes like glutamine synthetase and aspartate aminotransferase to maintain nitrogen and energy balance in cells. Glutamine synthetase helps convert the ammonia released into glutamine which is vital for cellular function.

Associated diseases and disorders

GLUD1 is linked to hyperinsulinism/hyperammonemia syndrome caused by mutations that lead to dysregulated insulin secretion. Disruptions in GLUD2 activity have implications in neurological disorders such as schizophrenia where it affects glutamate signaling. Both enzymes interact with insulin pathways and can influence disease states through these interactions.

Product promise

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4 product images

  • Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027), expandable thumbnail

    Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution

    All lanes: Mouse testis whole cell lysate at 50 µg

    Predicted band size: 61 kDa

  • Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027), expandable thumbnail

    Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution

    Lane 1: 293T whole cell lysate at 30 µg

    Lane 2: A431 whole cell lysate at 30 µg

    Lane 3: HeLa whole cell lysate at 30 µg

    Lane 4: HepG2 whole cell lysate at 30 µg

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (ab154027), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (ab154027)

    Immunohistochemical analysis of paraffin-embedded Human Hepatoma labeling GLUD1 + GLUD2 with ab154027 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody (ab154027), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody (ab154027)

    Immunofluorescent analysis of methanol-fixed HeLa cells labeling GLUD1 + GLUD2 with ab154027 antibody at 1/500 dilution. Bottom figure: cells costained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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