AB154027

Anti-GLUD1 + GLUD2 antibody

Name: Anti-GLUD1 + GLUD2 antibody (ab154027) | Abcam
Brand: Abcam Limited
SKU: AB154027

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(2 Publications)

Rabbit Polyclonal GLUD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLUD2 aa 250 to C-terminus.

View Alternative Names

GLUD, GLUD1, GDH 1, GLUDP1, GLUD2, GDH 2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (AB154027)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (AB154027)

Immunohistochemical analysis of paraffin-embedded Human Hepatoma labeling GLUD1 + GLUD2 with ab154027 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody (AB154027)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody (AB154027)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling GLUD1 + GLUD2 protein at mitochondria using ab154027 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

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Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution

All lanes:

Rat testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 61 kDa

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Unknown

Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution

All lanes:

Mouse testis whole cell lysate at 50 µg

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GLUD2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49448

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD1 and GLUD2 also known as glutamate dehydrogenase 1 and 2 are enzymes that catalyze the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. The mass of GLUD1 is approximately 61 kDa and it is expressed in multiple tissues with high levels in the liver brain and pancreas. GLUD2 is expressed mainly in the brain. These enzymes are integral for regulating amino acid metabolism and are activated by ADP and inhibited by GTP.

Biological function summary

GLUD1 and GLUD2 influence cellular energy homeostasis by modulating levels of glutamate and alpha-ketoglutarate. They function as homodimers or heterodimers and play a role in neurotransmitter cycling in neural tissues. This involvement in neurotransmitter metabolism is significant as they ensure glutamate levels remain balanced preventing excitotoxicity.

Pathways

GLUD1 and GLUD2 participate in the urea cycle and glutamate metabolism. They work alongside other enzymes like glutamine synthetase and aspartate aminotransferase to maintain nitrogen and energy balance in cells. Glutamine synthetase helps convert the ammonia released into glutamine which is vital for cellular function.

GLUD1 is linked to hyperinsulinism/hyperammonemia syndrome caused by mutations that lead to dysregulated insulin secretion. Disruptions in GLUD2 activity have implications in neurological disorders such as schizophrenia where it affects glutamate signaling. Both enzymes interact with insulin pathways and can influence disease states through these interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

See full target information GLUD1

Additional targets

GLUD2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34639216

2021

Differences in Glycolysis and Mitochondrial Respiration between Cytotrophoblast and Syncytiotrophoblast In-Vitro: Evidence for Sexual Dimorphism.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Bucher,Leena Kadam,Kylia Ahuna,Leslie Myatt

Cell systems 11:25-41.e9 PubMed32634384

2020

A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program.

Applications

Unspecified application

Species

Unspecified reactive species

Celia Alda-Catalinas,Danila Bredikhin,Irene Hernando-Herraez,Fátima Santos,Oana Kubinyecz,Mélanie A Eckersley-Maslin,Oliver Stegle,Wolf Reik

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GLUD1 + GLUD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (AB154027)

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody (AB154027)

Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027)

Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027)

Western blot - Anti-GLUD1 + GLUD2 antibody (AB154027)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise