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AB249357

Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal GLUD1 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Rat, Human, Mouse samples.

View Alternative Names

GLUD, GLUD1, GDH 1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

ab166618 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

ab166618 showing +ve staining in Human normal tonsil.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

ab166618 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

ab166618 showing +ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)
  • WB

Unknown

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (AB249357)

This data was developed using ab166618, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (<a href='/en-us/products/primary-antibodies/glud1-glud2-antibody-epr11369b-ab166618'>ab166618</a>) at 1/10000 dilution

Lane 1:

Human fetal liver lysates at 10 µg

Lane 2:

HepG2 lysates at 10 µg

Lane 3:

HeLa lysates at 10 µg

Lane 4:

293T lysates at 10 µg

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11369(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody immunogen shows 100% identity to human GLUD2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab249357 is the carrier-free version of ab166618.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD1 and GLUD2 also known as glutamate dehydrogenases are enzymes that play roles in amino acid metabolism. GLUD1 enzyme weighs approximately 56 kDa. These enzymes convert glutamate to α-ketoglutarate and ammonia thereby regulating amino acid turnover and nitrogen metabolism. GLUD1 and GLUD2 are expressed in mitochondria across numerous tissues but are highly represented in the liver and brain tissues. Mouse GLUD1 is often studied to understand these functions through model organisms.
Biological function summary

These enzymes participate in critical processes like neurotransmitter regulation and energy production. GLUD1 and GLUD2 provide ammonia and α-ketoglutarate necessary for the Krebs cycle connecting amino acid catabolism to energy production. Although not typically part of a larger complex their activity modulates key metabolic pathways essential for cellular energy.

Pathways

GLUD1 and GLUD2 play central roles in nitrogen and tricarboxylic acid (TCA) cycles. Within these cycles they facilitate interactions with proteins like AMPK linking amino acid metabolism to cellular energy sensing. They influence energy homeostasis by modulating NADH and NADPH production further integrating with metabolic control pathways like gluconeogenesis.

GLUD1 and GLUD2 have associations with hyperinsulinism/hyperammonemia syndrome and neurodegenerative disorders such as Alzheimer’s disease. In the context of hyperinsulinism faulty regulation of GLUD1 can lead to excessive insulin production. In neurodegenerative conditions these proteins by altering neurotransmitter levels possibly interact with proteins such as amyloid-β contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).
See full target information GLUD1

Additional targets

GLUD2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com