Rabbit Recombinant Monoclonal GLUD1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 10 publications.
Images
![Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--western-blot-img61498.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88661.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)")
![Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunocytochemistry-immunofluorescence-img2706.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)")
![Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--flow-cytometry-intracellular-img13751.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103108.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)")
Publications
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- Cell reports. Medicine 4:1010262023Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinchen Tang,Xiang-Hong Xu,Sha Xu,Zeying Liu,Qizhi He,Wenxuan Li,Jiaxue Sun,Wen Shuai,Jingwen Mao,Jian-Yuan Zhao,Liping JinPubMed 37137303
- Frontiers in immunology 13:9546162022Identification and validation of an immune signature associated with EMT and metabolic reprogramming for predicting prognosis and drug response in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao Zhang,Yongbo Yu,Peng Li,Meilan Wang,Wei Jiao,Ye Liang,Haitao NiuPubMed 35958586
- Molecular immunology 147:101-1142022TBK1 participates in glutaminolysis by mediating the phosphorylation of RIPK3 to promote endotoxin tolerance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLehan Pan,Lian Yang,Zhujun Yi,Wenfeng Zhang,Jianping GongPubMed 35533409
- Developmental cell 56:2329-2347.e62021Metabolic plasticity drives development during mammalian embryogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark S Sharpley,Fangtao Chi,Johanna Ten Hoeve,Utpal BanerjeePubMed 34428399
- Theranostics 11:5650-56742021Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi WangPubMed 33897873
- OncoTargets and therapy 13:7317-73272020Methylcrotonoyl-CoA Carboxylase 2 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Prostate Cancer Cells Through Regulating GLUD1-P38 MAPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianwen He,Yunhua Mao,Wentao Huang,Mingzhao Li,Huimin Zhang,Yunhao Qing,Shuo Lu,Hengjun Xiao,Ke LiPubMed 32801758
- International journal of molecular medicine 44:2189-22002019Glutamine affects T24 bladder cancer cell proliferation by activating STAT3 through ROS and glutaminolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNingchuan Sun et. al.PubMed 31661119
- Breast cancer research and treatment 174:79-912018Glutamate dehydrogenase (GLUD1) expression in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMadeleine L Craze et. al.PubMed 30470977
- Journal of cellular and molecular medicine 21:2896-29082017HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.
- Experimental biology and medicine (Maywood, N.J.) 242:945-9522017Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia Vázquez-Martínez et. al.PubMed 28440738
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Predicted | Expected | Predicted | Predicted |
| Rat | Not recommended | Predicted | Expected | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed:11032875, PubMed:11254391, PubMed:16023112, PubMed:16959573). Plays a role in insulin homeostasis (PubMed:11297618, PubMed:9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).
Additional Targets
GLUD2
Alternative names
GLUD, GLUD1, GDH 1
Recommended products
Rabbit Recombinant Monoclonal GLUD1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11370
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLUD1 and GLUD2 also known as glutamate dehydrogenases are enzymes that play roles in amino acid metabolism. GLUD1 enzyme weighs approximately 56 kDa. These enzymes convert glutamate to α-ketoglutarate and ammonia thereby regulating amino acid turnover and nitrogen metabolism. GLUD1 and GLUD2 are expressed in mitochondria across numerous tissues but are highly represented in the liver and brain tissues. Mouse GLUD1 is often studied to understand these functions through model organisms.
- Biological function summary
These enzymes participate in critical processes like neurotransmitter regulation and energy production. GLUD1 and GLUD2 provide ammonia and α-ketoglutarate necessary for the Krebs cycle connecting amino acid catabolism to energy production. Although not typically part of a larger complex their activity modulates key metabolic pathways essential for cellular energy.
- Pathways
GLUD1 and GLUD2 play central roles in nitrogen and tricarboxylic acid (TCA) cycles. Within these cycles they facilitate interactions with proteins like AMPK linking amino acid metabolism to cellular energy sensing. They influence energy homeostasis by modulating NADH and NADPH production further integrating with metabolic control pathways like gluconeogenesis.
- Associated diseases and disorders
GLUD1 and GLUD2 have associations with hyperinsulinism/hyperammonemia syndrome and neurodegenerative disorders such as Alzheimer’s disease. In the context of hyperinsulinism faulty regulation of GLUD1 can lead to excessive insulin production. In neurodegenerative conditions these proteins by altering neurotransmitter levels possibly interact with proteins such as amyloid-β contributing to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--western-blot-img61498.jpg "Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
All lanes: Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
SecondaryAll lanes: HRP labeled goat anti-rabbit at 1/2000 dilution
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88661.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
ab168352 showing +ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunocytochemistry-immunofluorescence-img2706.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
Immunofluorescent analysis of HepG2 cells labeling Glutamate Dehydrogenase with ab168352 at 1/50 dilution.
![Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--flow-cytometry-intracellular-img13751.jpg "Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103108.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
ab168352 showing +ve staining in Human normal uterus.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83539.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
ab168352 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125826.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83879.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83539.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125826.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352), expandable thumbnail](https://content.abcam.com/products/images/glud1-glud2-antibody-epr11370-ab168352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83879.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
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