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AB168352

Anti-GLUD1 + GLUD2 antibody [EPR11370]

3

(1 Review)

|

(13 Publications)

Rabbit Recombinant Monoclonal GLUD1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

GLUD, GLUD1, GDH 1

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

ab168352 showing +ve staining in Human normal uterus.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

ab168352 showing +ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

Immunofluorescent analysis of HepG2 cells labeling Glutamate Dehydrogenase with ab168352 at 1/50 dilution.

Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

ab168352 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)
  • WB

Unknown

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

All lanes:

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • Carrier free

    Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free

  • 660 APC

    APC Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • HRP

    HRP Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 578 PE

    PE Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GLUD1 + GLUD2 antibody [EPR11370]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11370

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD1 and GLUD2 also known as glutamate dehydrogenases are enzymes that play roles in amino acid metabolism. GLUD1 enzyme weighs approximately 56 kDa. These enzymes convert glutamate to α-ketoglutarate and ammonia thereby regulating amino acid turnover and nitrogen metabolism. GLUD1 and GLUD2 are expressed in mitochondria across numerous tissues but are highly represented in the liver and brain tissues. Mouse GLUD1 is often studied to understand these functions through model organisms.
Biological function summary

These enzymes participate in critical processes like neurotransmitter regulation and energy production. GLUD1 and GLUD2 provide ammonia and α-ketoglutarate necessary for the Krebs cycle connecting amino acid catabolism to energy production. Although not typically part of a larger complex their activity modulates key metabolic pathways essential for cellular energy.

Pathways

GLUD1 and GLUD2 play central roles in nitrogen and tricarboxylic acid (TCA) cycles. Within these cycles they facilitate interactions with proteins like AMPK linking amino acid metabolism to cellular energy sensing. They influence energy homeostasis by modulating NADH and NADPH production further integrating with metabolic control pathways like gluconeogenesis.

GLUD1 and GLUD2 have associations with hyperinsulinism/hyperammonemia syndrome and neurodegenerative disorders such as Alzheimer’s disease. In the context of hyperinsulinism faulty regulation of GLUD1 can lead to excessive insulin production. In neurodegenerative conditions these proteins by altering neurotransmitter levels possibly interact with proteins such as amyloid-β contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).
See full target information GLUD1

Additional targets

GLUD2
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers

Cell reports. Medicine 4:101026 PubMed37137303

2023

Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Linchen Tang,Xiang-Hong Xu,Sha Xu,Zeying Liu,Qizhi He,Wenxuan Li,Jiaxue Sun,Wen Shuai,Jingwen Mao,Jian-Yuan Zhao,Liping Jin

Frontiers in immunology 13:954616 PubMed35958586

2022

Identification and validation of an immune signature associated with EMT and metabolic reprogramming for predicting prognosis and drug response in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Zhang,Yongbo Yu,Peng Li,Meilan Wang,Wei Jiao,Ye Liang,Haitao Niu

Molecular immunology 147:101-114 PubMed35533409

2022

TBK1 participates in glutaminolysis by mediating the phosphorylation of RIPK3 to promote endotoxin tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Lehan Pan,Lian Yang,Zhujun Yi,Wenfeng Zhang,Jianping Gong

Developmental cell 56:2329-2347.e6 PubMed34428399

2021

Metabolic plasticity drives development during mammalian embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mark S Sharpley,Fangtao Chi,Johanna Ten Hoeve,Utpal Banerjee

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

The international journal of biochemistry & cell b :105935 PubMed33529714

2021

The utilisation of glutamine and glucose by a 3-D tumour model trapped in quiescence.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Smith,David De Souza,Dedreia Tull,Malcolm McConville,Andrea Pellagatti,Jacqueline Boultwood,Mary Board,Richard Callaghan

OncoTargets and therapy 13:7317-7327 PubMed32801758

2020

Methylcrotonoyl-CoA Carboxylase 2 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Prostate Cancer Cells Through Regulating GLUD1-P38 MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwen He,Yunhua Mao,Wentao Huang,Mingzhao Li,Huimin Zhang,Yunhao Qing,Shuo Lu,Hengjun Xiao,Ke Li

International journal of molecular medicine 44:2189-2200 PubMed31661119

2019

Glutamine affects T24 bladder cancer cell proliferation by activating STAT3 through ROS and glutaminolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ningchuan Sun,Ye Liang,Yuanbin Chen,Liping Wang,Dan Li,Zhijuan Liang,Lijiang Sun,Yonghua Wang,Haitao Niu

American journal of physiology. Lung cellular and 316:L723-L737 PubMed30652491

2019

Mesenchymal stromal cell-derived exosomes improve mitochondrial health in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Hogan,Maria Pia Rodriguez Salazar,John Cheadle,Rachel Glenn,Carolina Medrano,Thomas H Petersen,Roger M Ilagan
View all publications
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