AB118891

Anti-GluK1 antibody

Name: Anti-GluK1 antibody (ab118891) | Abcam
Brand: Abcam Limited
SKU: AB118891

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(3 Publications)

Rabbit Polyclonal GluK1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GRIK1.

View Alternative Names

GLUR5, GRIK1, GluK1, Excitatory amino acid receptor 3, Glutamate receptor 5, EAA3, GluR-5, GluR5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (AB118891)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (AB118891)

ab118891 at 5 μg/ml staining GluK1 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-GluK1 antibody (AB118891)

Lane 1:

Western blot - Anti-GluK1 antibody (ab118891) at 0.5 µg/mL

Lane 2:

Western blot - Anti-GluK1 antibody (ab118891) at 1 µg/mL

All lanes:

Rat Brain Tissue Lysate

Predicted band size: 104 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GRIK1. The exact immunogen used to generate this antibody is proprietary information.

P39086

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GluK1 also known as GluR5 is a subunit of the kainate-type ionotropic glutamate receptor. It has a mass of approximately 99 kDa. This protein is expressed in various regions of the central nervous system including the hippocampus and cerebral cortex. GluK1 operates as a ligand-gated ion channel mediating synaptic transmission by allowing the influx of sodium and to a lesser extent calcium ions upon activation by kainate.

Biological function summary

GluK1 plays a role in modulating neurotransmission and synaptic plasticity. As part of the kainate receptor complex it contributes to excitatory signaling in the brain. Other components of this complex include GluK2 GluK3 GluK4 and GluK5 subunits. This receptor complex influences neuron excitability and has a hand in shaping synaptic networks by regulating glutamate release from presynaptic terminals.

Pathways

GluK1 participates in glutamatergic neurotransmission and the regulation of synaptic strength. This protein interacts with other ionotropic glutamate receptors such as AMPA and NMDA receptors within these pathways. These interactions affect long-term potentiation (LTP) and long-term depression (LTD) which are essential for learning and memory processes in the brain.

Dysfunction or dysregulation of GluK1 has been linked to neurological disorders such as temporal lobe epilepsy and schizophrenia. Abnormal GluK1 activity can influence neural excitability which is connected to the onset and progression of epileptic episodes. Moreover changes in GluK1 function may be associated with altered glutamatergic signaling in schizophrenia alongside proteins like NMDAR highlighting its importance in neuropsychiatric conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.. Isoform 2. Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid.

See full target information GRIK1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 16:1322750 PubMed38249292

2024

Delving into the significance of the His289Tyr single-nucleotide polymorphism in the glutamate ionotropic receptor kainate-1 () gene of a genetically audiogenic seizure model.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra M Díaz-Rodríguez,M Javier Herrero-Turrión,Carlos García-Peral,Ricardo Gómez-Nieto

International journal of molecular sciences 24: PubMed38069190

2023

Enhanced Membrane Incorporation of H289Y Mutant GluK1 Receptors from the Audiogenic Seizure-Prone GASH/Sal Model: Functional and Morphological Impacts on Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra M Díaz-Rodríguez,Isabel Ivorra,Javier Espinosa,Celia Vegar,M Javier Herrero-Turrión,Dolores E López,Ricardo Gómez-Nieto,Armando Alberola-Die

Oncotarget 8:106454-106467 PubMed29290962

2017

Pharmacogenetics of response to neoadjuvant paclitaxel treatment for locally advanced breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Andric C Perez-Ortiz,Israel Ramírez,Juan C Cruz-López,Cynthia Villarreal-Garza,Alexandra Luna-Angulo,Esmeralda Lira-Romero,Salvador Jiménez-Chaidez,José Díaz-Chávez,Juan A Matus-Santos,Laura Sánchez-Chapul,Patricia Mendoza-Lorenzo,Francisco J Estrada-Mena

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GluK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (AB118891)

Western blot - Anti-GluK1 antibody (AB118891)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise