AB67316

Anti-GluK1 antibody

Name: Anti-GluK1 antibody (ab67316) | Abcam
Brand: Abcam Limited
SKU: AB67316
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GluK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GRIK1.

View Alternative Names

GLUR5, GRIK1, GluK1, Excitatory amino acid receptor 3, Glutamate receptor 5, EAA3, GluR-5, GluR5

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (AB67316)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (AB67316)

Paraffin-embedded mouse brain tissue stained for GluK1 using ab67316 at 5 μg/ml in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GluK1 antibody (AB67316)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GluK1 antibody (AB67316)

Immunofluorescence of GluK1 in Mouse Brain cells using ab67316 at 20 ug/ml.

Unknown

Western blot - Anti-GluK1 antibody (AB67316)

Lane 1:

Western blot - Anti-GluK1 antibody (ab67316) at 0.5 µg/mL

Lane 2:

Western blot - Anti-GluK1 antibody (ab67316) at 1 µg/mL

Lane 3:

Western blot - Anti-GluK1 antibody (ab67316) at 2 µg/mL

All lanes:

P815 cell lysate at 15 µg

Predicted band size: 104 kDa

Observed band size: 104 kDa,95 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human GRIK1. The exact immunogen used to generate this antibody is proprietary information.

P39086

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GluK1 also known as GluR5 is a subunit of the kainate-type ionotropic glutamate receptor. It has a mass of approximately 99 kDa. This protein is expressed in various regions of the central nervous system including the hippocampus and cerebral cortex. GluK1 operates as a ligand-gated ion channel mediating synaptic transmission by allowing the influx of sodium and to a lesser extent calcium ions upon activation by kainate.

Biological function summary

GluK1 plays a role in modulating neurotransmission and synaptic plasticity. As part of the kainate receptor complex it contributes to excitatory signaling in the brain. Other components of this complex include GluK2 GluK3 GluK4 and GluK5 subunits. This receptor complex influences neuron excitability and has a hand in shaping synaptic networks by regulating glutamate release from presynaptic terminals.

Pathways

GluK1 participates in glutamatergic neurotransmission and the regulation of synaptic strength. This protein interacts with other ionotropic glutamate receptors such as AMPA and NMDA receptors within these pathways. These interactions affect long-term potentiation (LTP) and long-term depression (LTD) which are essential for learning and memory processes in the brain.

Dysfunction or dysregulation of GluK1 has been linked to neurological disorders such as temporal lobe epilepsy and schizophrenia. Abnormal GluK1 activity can influence neural excitability which is connected to the onset and progression of epileptic episodes. Moreover changes in GluK1 function may be associated with altered glutamatergic signaling in schizophrenia alongside proteins like NMDAR highlighting its importance in neuropsychiatric conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.. Isoform 2. Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid.

See full target information GRIK1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brain structure & function 227:1599-1614 PubMed35103859

2022

Properties of the epileptiform activity in the cingulate cortex of a mouse model of LIS1 dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

E Domínguez-Sala,A Andreu-Cervera,P Martín-Climent,R Murcia-Ramón,S Martínez,Emilio Geijo-Barrientos

Bioscience reports 40: PubMed32285917

2020

MiR-139-5p influences hepatocellular carcinoma cell invasion and proliferation capacities via decreasing SLITRK4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jisiguleng Wu,Tong Zhang,Yubo Chen,Sigaowa Ha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GluK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (AB67316)

Immunocytochemistry/ Immunofluorescence - Anti-GluK1 antibody (AB67316)

Western blot - Anti-GluK1 antibody (AB67316)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise