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Rabbit Recombinant Monoclonal GluN2D antibody. Suitable for WB and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-GluN2D antibody [EPR28378-56] (AB314664), expandable thumbnail
  • Western blot - Anti-GluN2D antibody [EPR28378-56] (AB314664), expandable thumbnail
  • Western blot - Anti-GluN2D antibody [EPR28378-56] (AB314664), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow CytICC/IFIHC-FrIHC-PIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:1385220). Participates in synaptic plasticity for learning and memory formation (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1385220, PubMed:7790891). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal GluN2D antibody. Suitable for WB and reacts with Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28378-56

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GluN2D also known as GRIN2D or NR2D is a subunit of the NMDA (N-methyl-D-aspartate) receptor which plays an essential role in excitatory neurotransmission in the brain. It has a molecular mass of about 130 kDa. This protein is primarily expressed in the central nervous system including the neocortex hippocampus and thalamus. GluN2D's role within the NMDA receptor complex involves modulating synaptic plasticity learning and memory processes by binding with calcium ions.

Biological function summary

GluN2D contributes to the complexity of neuronal communication and signaling. It is an integral component of the NMDA receptor complex which is a heterotetramer consisting of GluN1 GluN2 and occasionally GluN3 subunits. The GluN2D subunit influences the receptor's biophysical and pharmacological properties affecting the duration and amplitude of the excitatory postsynaptic current. Through these functions GluN2D plays a role in synaptic transmission and neuroplasticity impacting how neurons adapt to various stimuli and experiences.

Pathways

GluN2D plays significant roles in synaptic signaling and neurodevelopmental pathways. In synaptic signaling GluN2D modulates calcium ion influx which is necessary for activating intracellular pathways involving proteins like CaMKII critical for long-term potentiation and memory formation. In development its involvement in neuronal differentiation and maturation interacts with proteins like PSD-95 which are essential for organizing synaptic architecture and function. These pathways highlight the functional importance of GluN2D in both synaptic and developmental contexts.

Associated diseases and disorders

GluN2D is associated with neurological and psychiatric conditions such as epilepsy and schizophrenia. Variations in GRIN2D gene expression or function can disrupt NMDA receptor activity leading to an imbalance in excitatory and inhibitory neurotransmission in the central nervous system. This imbalance can contribute to seizure generation in epilepsy or the symptoms observed in schizophrenia. Additionally GluN2D interactions with proteins like DLG4 associated with postsynaptic density further influence these disease mechanisms offering potential therapeutic targets for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664), expandable thumbnail

    Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle.
    The identity of the bands between 75 kDa and 37 kDa are unknown.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664) at 1/1000 dilution

    Lane 1: Rat cerebellum tissue lysate at 20 µg

    Lane 2: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 165 kDa

    Exposure time: 158s

  • Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664), expandable thumbnail

    Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle.
    The identity of the bands between 75 kDa and 25 kDa are unknown.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 165 kDa

    Exposure time: 180s

  • Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664), expandable thumbnail

    Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identity of the bands between 75 kDa and 25 kDa are unknown.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
    In Western blot, Anti-Integrin alpha V antibody [EPR16800] (Anti-Integrin alpha V antibody [EPR16800] ab179475) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-GluN2D antibody [EPR28378-56] (ab314664) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse brain membrane fraction at 20 µg

    Lane 3: Mouse brain non-membrane fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 165 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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