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AB314665

Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free

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Rabbit Recombinant Monoclonal GluN2D antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

GluN2D, Grin2d, Glutamate [NMDA] receptor subunit epsilon-4, N-methyl D-aspartate receptor subtype 2D, NMDAR2D, NR2D

3 Images
Western blot - Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free (AB314665)
  • WB

Supplier Data

Western blot - Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free (AB314665)

This data was developed using ab314664, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle. The identity of the bands between 75 kDa and 37 kDa are unknown. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-GluN2D antibody [EPR28378-56] (<a href='/en-us/products/primary-antibodies/glun2d-antibody-epr28378-56-ab314664'>ab314664</a>) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 165 kDa

false

Exposure time: 158s

Western blot - Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free (AB314665)
  • WB

Supplier Data

Western blot - Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free (AB314665)

This data was developed using ab314664, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle. The identity of the bands between 75 kDa and 25 kDa are unknown. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-GluN2D antibody [EPR28378-56] (<a href='/en-us/products/primary-antibodies/glun2d-antibody-epr28378-56-ab314664'>ab314664</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 165 kDa

false

Exposure time: 180s

Western blot - Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free (AB314665)
  • WB

Supplier Data

Western blot - Anti-GluN2D antibody [EPR28378-56] - BSA and Azide free (AB314665)

This data was developed using ab314664, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the bands between 75 kDa and 25 kDa are unknown. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution. In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-GluN2D antibody [EPR28378-56] (<a href='/en-us/products/primary-antibodies/glun2d-antibody-epr28378-56-ab314664'>ab314664</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse brain membrane fraction at 20 µg

Lane 3:

Mouse brain non-membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 165 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28378-56

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314665 is the carrier-free version of ab314664.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GluN2D also known as GRIN2D or NR2D is a subunit of the NMDA (N-methyl-D-aspartate) receptor which plays an essential role in excitatory neurotransmission in the brain. It has a molecular mass of about 130 kDa. This protein is primarily expressed in the central nervous system including the neocortex hippocampus and thalamus. GluN2D's role within the NMDA receptor complex involves modulating synaptic plasticity learning and memory processes by binding with calcium ions.
Biological function summary

GluN2D contributes to the complexity of neuronal communication and signaling. It is an integral component of the NMDA receptor complex which is a heterotetramer consisting of GluN1 GluN2 and occasionally GluN3 subunits. The GluN2D subunit influences the receptor's biophysical and pharmacological properties affecting the duration and amplitude of the excitatory postsynaptic current. Through these functions GluN2D plays a role in synaptic transmission and neuroplasticity impacting how neurons adapt to various stimuli and experiences.

Pathways

GluN2D plays significant roles in synaptic signaling and neurodevelopmental pathways. In synaptic signaling GluN2D modulates calcium ion influx which is necessary for activating intracellular pathways involving proteins like CaMKII critical for long-term potentiation and memory formation. In development its involvement in neuronal differentiation and maturation interacts with proteins like PSD-95 which are essential for organizing synaptic architecture and function. These pathways highlight the functional importance of GluN2D in both synaptic and developmental contexts.

GluN2D is associated with neurological and psychiatric conditions such as epilepsy and schizophrenia. Variations in GRIN2D gene expression or function can disrupt NMDA receptor activity leading to an imbalance in excitatory and inhibitory neurotransmission in the central nervous system. This imbalance can contribute to seizure generation in epilepsy or the symptoms observed in schizophrenia. Additionally GluN2D interactions with proteins like DLG4 associated with postsynaptic density further influence these disease mechanisms offering potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 1385220). Participates in synaptic plasticity for learning and memory formation (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 1385220, PubMed : 7790891). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (By similarity).
See full target information Grin2d
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Product promise

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