Rabbit Recombinant Monoclonal NR3A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--western-blot-img400915.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)")
![Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--western-blot-img400917.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)")
![Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--western-blot-img400916.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400949.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400950.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes The antibody exhibits low sensitivity when detecting rat samples. It is recommended to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Component of a non-conventional N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with low calcium permeability and low voltage-dependent block by Mg(2+). During the development of neural circuits, participates in the synaptic refinement period, restricting spine maturation and growth (By similarity). Forms glutamatergic receptor complexes with GluN1 and GluN2 subunits which are activated by glycine binding to the GluN1 and GluN3 subunits and L-glutamate binding to GluN2 subunits (By similarity). Forms excitatory glycinergic receptor complexes with GluN1 alone which are activated by glycine binding to the GluN1 and GluN3 subunits (By similarity). GluN3A subunit also binds D-serine (By similarity). Each GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (By similarity). By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed:24297929).
Additional Targets
Grin3b, Grin3b, Grin3b
Alternative names
Kiaa1973, Grin3a, GluN3A, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NMDAR-L, NMDAR3A, NR3A
Recommended products
Rabbit Recombinant Monoclonal NR3A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25287-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NR3A and NR3B also known as GluN3A and GluN3B are subunits of the NMDA receptor part of the ionotropic glutamate receptor family. They contain a molecular mass of approximately 129 kDa for NR3A and 130 kDa for NR3B. These subunits are expressed mainly in neurons within the central nervous system especially in the developing brain. Alongside the more common NR1 and NR2 subunits NR3 subunits play a unique regulatory role in NMDA receptor function and pharmacology.
- Biological function summary
NR3A and NR3B modulate neuronal excitability by disrupting ion flow through the NMDA receptor channel acting as glycine-binding subunits. They integrate into NMDA receptor complexes with NR1 and NR2 subunits that form the receptor’s functional core. By altering calcium permeability and synaptic plasticity these subunits impact processes like learning and memory. The presence of NR3 subunits in the complex influences receptor properties reducing the excitatory response.
- Pathways
NR3A and NR3B engage in the synaptic signaling and excitatory neurotransmission pathways which heavily depend on NMDA receptors. These pathways involve proteins such as NR1 and NR2 with which NR3 subunits interact to modulate synaptic transmission. The regulation of calcium influx and downstream signaling pathways including those impacting synaptic strength and plasticity is vital for various neuronal functions.
- Associated diseases and disorders
NR3A and NR3B influence neurodevelopmental and neurodegenerative conditions. Schizophrenia and Alzheimer's disease show links to dysregulated NMDA receptor activity with alterations in NR3 subunit expression playing a role. Research suggests that NR3-related modulation affects disease pathology and interactions with NR1 and NR2 subunits contribute to abnormal receptor function linked to these disorders. Studies focus on how targeting NR3 subunits may offer therapeutic avenues in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--western-blot-img400915.jpg "Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Negative controls: heart, spleen (PMID: 7472412)
Samples are non-boiled as boiling may cause protein aggregates.
All lanes: Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534) at 1/1000 dilution
Lane 1: Rat thalamus tissue lysate 20 µg
Lane 2: Rat P0 brain tissue lysate 20 µg
Lane 3: Rat heart tissue lysate 20 µg
Lane 4: Rat spleen tissue lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 130 kDa
Exposure time: 3min
![Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--western-blot-img400917.jpg "Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody reacts with rat GluN3A and GluN3B.
Samples are non-boiled as boiling may cause protein aggregates.All lanes: Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a his tag, whole cell lysate, 20 µg
Lane 2: HEK-293T cells transfected with a rat GluN3A expression vector containi a his tag, whole cell lysate, 20 µg
Lane 3: HEK-293T cells transfected with a rat GluN3B expression vector containi a his tag, whole cell lysate, 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 130 kDa, 125 kDa
Exposure time: 15s
![Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--western-blot-img400916.jpg "Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative controls: heart, spleen (PMID: 7472412)
Samples are non-boiled as boiling may cause protein aggregates.All lanes: Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534) at 1/1000 dilution
Lane 1: Mouse thalamus tissue lysate 20 µg
Lane 2: Mouse P1 brain tissue lysate 20 µg
Lane 3: Mouse heart tissue lysate 20 µg
Lane 4: Mouse spleen tissue lysate 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 130 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400949.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GluN3A+GluN3B with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 at 1/200 (2.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Postive staining on mouse cerebrum (PMID: 7472412).The section was incubated with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400950.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GluN3A+GluN3B with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 at 1/200 (2.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Postive staining on rat cerebrum (PMID: 7472412).The section was incubated with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400951.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling GluN3A+GluN3B with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 at 1/200 (2.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: No staining on mouse cardiac muscle.The section was incubated with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400952.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
This data was developed using Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling GluN3A+GluN3B with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 at 1/200 (2.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: No staining on rat cardiac muscle.The section was incubated with Anti-GluN3A+GluN3B antibody [EPR25287-45] ab302534 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400951.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535), expandable thumbnail](https://content.abcam.com/products/images/glun3aglun3b-antibody-epr25287-45-bsa-and-azide-free-ab302535--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400952.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)")
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