Rabbit Polyclonal GLUT9 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC2A9 aa 450 to C-terminus.
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Publications
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- JCI insight 8:2023GLUT1 is redundant in hypoxic and glycolytic nucleus pulposus cells of the intervertebral disc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShira N Johnston,Elizabeth S Silagi,Vedavathi Madhu,Duc H Nguyen,Irving M Shapiro,Makarand V RisbudPubMed 36917198
- Scientific reports 12:180862022Exercise serum regulates uric acid transporters in normal rat kidney cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongye Jiang,Jianmin Cao,Hao Su,Hui Cao,Zeyuan Sun,Haoze Jiang,Yanjun FanPubMed 36302802
- Frontiers in pharmacology 12:7871252022Extracts of (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEldiza Puji Rahmi,Endang Kumolosasi,Juriyati Jalil,Fhataheya Buang,Jamia Azdina JamalPubMed 35095497
- Pharmaceutical biology 59:175-1822021Inosine induces acute hyperuricaemia in rhesus monkey () as a potential disease animal model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Hong Tang,Chen-Yun Wang,Xi Huang,Hong-Kun Yi,Zhe-Li Li,Kai-Li Ma,You-Song Ye,Jian-Wen ZhangPubMed 33715593
- Molecular medicine reports 20:1645-16542019Urate transport capacity of glucose transporter 9 and urate transporter 1 in cartilage chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingqing Zhang et. al.PubMed 31257523
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLC2A9 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRM0
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
High-capacity urate transporter, which may play a role in the urate reabsorption by proximal tubules (PubMed:18327257, PubMed:18701466, PubMed:22647630, PubMed:28083649, PubMed:36749388). May have a residual high-affinity, low-capacity glucose and fructose transporter activity (PubMed:18327257, PubMed:18701466, PubMed:18842065). Transports urate at rates 45- to 60-fold faster than glucose (PubMed:18842065). Does not transport galactose (PubMed:28083649). May mediate small uptake of adenine but not of other nucleobases (PubMed:22647630).
Alternative names
GLUT9, SLC2A9, Glucose transporter type 9, Urate transporter, GLUT-9
Recommended products
Rabbit Polyclonal GLUT9 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC2A9 aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLC2A9 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRM0
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLUT9 also known as SLC2A9 functions as a facilitative glucose transporter with a molecular weight of about 57 kDa. It is highly expressed in the kidneys and liver with lower levels of expression in other tissues such as the placenta and heart. GLUT9 facilitates the transport of uric acid glucose and fructose across the cell membrane playing an important role in cellular energy balance and metabolic processes.
- Biological function summary
In the human body this protein influences urate homeostasis by managing uric acid levels in the blood. GLUT9 operates as a homomeric protein meaning it forms a functional unit by itself rather than needing to associate with other proteins to function. It significantly affects renal reabsorption processes regulating the excretion and reabsorption of urate in the kidneys. By controlling these uric acid levels it plays an important part in preventing hyperuricemia and related complications.
- Pathways
GLUT9 contributes mainly to carbohydrate metabolism and urate transport pathways. Its role within the urate transport pathway links it to various other urate transporter proteins such as URAT1 which also participates in the regulation of uric acid excretion. In glucose metabolism GLUT9 works alongside other glucose transporters to maintain glucose levels linking it indirectly to pathways involving insulin response.
- Associated diseases and disorders
Elevated GLUT9 activity associates closely with conditions like gout and hyperuricemia. Due to its role in uric acid transport any dysfunction in GLUT9 can lead to increased serum uric acid contributing to gout development. Furthermore the interaction between GLUT9 and correlating proteins such as ABCG2 influences uric acid excretion processes connecting it to the pathological mechanisms underpinning these disorders. Researchers focus on these interactions to develop potential therapeutic strategies to manage related conditions effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (ab223470)
Paraffin-embedded human kidney tissue stained for GLUT9 using ab223470 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (ab223470)
Paraffin-embedded human ovarian cancer tissue stained for GLUT9 using ab223470 at 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-GLUT9 antibody (ab223470)
All lanes: Western blot - Anti-GLUT9 antibody (ab223470) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2: Mouse liver lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Observed band size: 59 kDa
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