AB223470

Anti-GLUT9 antibody

Name: Anti-GLUT9 antibody (ab223470) | Abcam
Brand: Abcam Limited
SKU: AB223470

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(9 Publications)

Rabbit Polyclonal GLUT9 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC2A9 aa 450 to C-terminus.

View Alternative Names

GLUT9, SLC2A9, Glucose transporter type 9, Urate transporter, GLUT-9

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (AB223470)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (AB223470)

Paraffin-embedded human kidney tissue stained for GLUT9 using ab223470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (AB223470)

Paraffin-embedded human ovarian cancer tissue stained for GLUT9 using ab223470 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-GLUT9 antibody (AB223470)

All lanes:

Western blot - Anti-GLUT9 antibody (ab223470) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

Mouse liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 58 kDa

Observed band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC2A9 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRM0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUT9 also known as SLC2A9 functions as a facilitative glucose transporter with a molecular weight of about 57 kDa. It is highly expressed in the kidneys and liver with lower levels of expression in other tissues such as the placenta and heart. GLUT9 facilitates the transport of uric acid glucose and fructose across the cell membrane playing an important role in cellular energy balance and metabolic processes.

Biological function summary

In the human body this protein influences urate homeostasis by managing uric acid levels in the blood. GLUT9 operates as a homomeric protein meaning it forms a functional unit by itself rather than needing to associate with other proteins to function. It significantly affects renal reabsorption processes regulating the excretion and reabsorption of urate in the kidneys. By controlling these uric acid levels it plays an important part in preventing hyperuricemia and related complications.

Pathways

GLUT9 contributes mainly to carbohydrate metabolism and urate transport pathways. Its role within the urate transport pathway links it to various other urate transporter proteins such as URAT1 which also participates in the regulation of uric acid excretion. In glucose metabolism GLUT9 works alongside other glucose transporters to maintain glucose levels linking it indirectly to pathways involving insulin response.

Elevated GLUT9 activity associates closely with conditions like gout and hyperuricemia. Due to its role in uric acid transport any dysfunction in GLUT9 can lead to increased serum uric acid contributing to gout development. Furthermore the interaction between GLUT9 and correlating proteins such as ABCG2 influences uric acid excretion processes connecting it to the pathological mechanisms underpinning these disorders. Researchers focus on these interactions to develop potential therapeutic strategies to manage related conditions effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

High-capacity urate transporter, which may play a role in the urate reabsorption by proximal tubules (PubMed : 18327257, PubMed : 18701466, PubMed : 22647630, PubMed : 28083649, PubMed : 36749388). May have a residual high-affinity, low-capacity glucose and fructose transporter activity (PubMed : 18327257, PubMed : 18701466, PubMed : 18842065). Transports urate at rates 45- to 60-fold faster than glucose (PubMed : 18842065). Does not transport galactose (PubMed : 28083649). May mediate small uptake of adenine but not of other nucleobases (PubMed : 22647630).

See full target information SLC2A9

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 14: PubMed40870677

2025

Yellow Pigments Ameliorate Hyperuricemia via Dual Mechanisms: Xanthine Oxidase Inhibition and Uric Acid Transporter Modulation (ABCG2, URAT1, and GLUT9).

Applications

Unspecified application

Species

Unspecified reactive species

Furong Xue,Renqin Zhu,Jiaxing Li,Zheng Liu,Lidan Niu,Wei Chen,Chengtao Wang,Jie Zheng

Foods (Basel, Switzerland) 14: PubMed39942117

2025

In Slico Screening and In Vitro Identification of Hyperuricemia-Inhibiting Peptides from .

Applications

Unspecified application

Species

Unspecified reactive species

Zexuan Xu,Miaoyu Gan,Weiliang Guan,Fang Tian,Yuxi Wang,Jinjie Zhang,Luyun Cai

iScience 27:111186 PubMed39524334

2024

Kaempferol attenuates hyperuricemia combined with gouty arthritis via urate transporters and NLRP3/NF-κB pathway modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Cantao Li,Wenjing Xu,Fenfen Li,Ying Hua,Changyu Xu,Chenxi Wu,Yihuan Wang,Xiaoxi Zhang,Daozong Xia

SLAS discovery : advancing life sciences R & D 29:100193 PubMed39522878

2024

The development of a novel high-throughput membrane potential assay and a solid-supported membrane (SSM)-based electrophysiological assay to study the pharmacological inhibition of GLUT9/SLC2A9 isoforms in a drug discovery program.

Applications

Unspecified application

Species

Unspecified reactive species

Antje Pommereau,Francesca Sassone,Alessandro Poli,Marcella De Silvestris,Lia Scarabottolo,Yasmin Zuschlag,Thomas Licher,Felix Bärenz

JCI insight 8: PubMed36917198

2023

GLUT1 is redundant in hypoxic and glycolytic nucleus pulposus cells of the intervertebral disc.

Applications

Unspecified application

Species

Unspecified reactive species

Shira N Johnston,Elizabeth S Silagi,Vedavathi Madhu,Duc H Nguyen,Irving M Shapiro,Makarand V Risbud

Scientific reports 12:18086 PubMed36302802

2022

Exercise serum regulates uric acid transporters in normal rat kidney cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongye Jiang,Jianmin Cao,Hao Su,Hui Cao,Zeyuan Sun,Haoze Jiang,Yanjun Fan

Frontiers in pharmacology 12:787125 PubMed35095497

2022

Extracts of (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Eldiza Puji Rahmi,Endang Kumolosasi,Juriyati Jalil,Fhataheya Buang,Jamia Azdina Jamal

Pharmaceutical biology 59:175-182 PubMed33715593

2021

Inosine induces acute hyperuricaemia in rhesus monkey () as a potential disease animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Hong Tang,Chen-Yun Wang,Xi Huang,Hong-Kun Yi,Zhe-Li Li,Kai-Li Ma,You-Song Ye,Jian-Wen Zhang

Molecular medicine reports 20:1645-1654 PubMed31257523

2019

Urate transport capacity of glucose transporter 9 and urate transporter 1 in cartilage chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Bingqing Zhang,Mengyuan Duan,Bo Long,Baozhong Zhang,Dongmei Wang,Yun Zhang,Jialin Chen,Xiaoming Huang,Yang Jiao,Lei Zhu,Xuejun Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GLUT9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (AB223470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUT9 antibody (AB223470)

Western blot - Anti-GLUT9 antibody (AB223470)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

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