Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) is a rabbit polyclonal antibody that is used to detect Glutamate Receptor 1 (AMPA subtype) in Western Blot, IHC-P. Suitable for Common marmoset, Mouse, Rat samples.
- Over 160 publications
- Trusted since 2006
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- Molecular psychiatry 29:686-7032023Interplay between hippocampal TACR3 and systemic testosterone in regulating anxiety-associated synaptic plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Natalia Wojtas,Marta Diaz-González,Nadezhda Stavtseva,Yuval Shoam,Poonam Verma,Assaf Buberman,Inbar Izhak,Aria Geva,Roi Basch,Alberto Ouro,Lucia Perez-Benitez,Uri Levy,Erika Borcel,Ángel Nuñez,Cesar Venero,Noa Rotem-Dai,Isana Veksler-Lublinsky,Shira KnafoPubMed 38135756
- Nature communications 14:79712023Enhanced TARP-γ8-PSD-95 coupling in excitatory neurons contributes to the rapid antidepressant-like action of ketamine in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Ge Xue,Jin-Gang He,Ling-Li Lu,Shi-Jie Song,Mei-Mei Chen,Fang Wang,Jian-Guo ChenPubMed 38042894
- Frontiers in cellular neuroscience 17:12114862023Neuroprotection by upregulation of the major histocompatibility complex class I (MHC I) in SOD1 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Laura M R Tomiyama,Luciana Politti Cartarozzi,Lilian de Oliveira Coser,Gabriela Bortolança Chiarotto,Alexandre L R OliveiraPubMed 37711512
- Journal of translational medicine 21:5672023Inhibiting NLRP3 inflammasome signaling pathway promotes neurological recovery following hypoxic-ischemic brain damage by increasing p97-mediated surface GluA1-containing AMPA receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxin Chen,Xiaohuan Li,Qian Xiong,Yehong Du,Man Luo,Lilin Yi,Yayan Pang,Xiuyu Shi,Yu Tian Wang,Zhifang DongPubMed 37620837
- JCI insight 8:2023MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianwei Ge,Zhiwei Xue,Shu Shu,Linjie Yu,Ruomeng Qin,Wenyuan Tao,Pinyi Liu,Xiaohong Dong,Zhen Lan,Xinyu Bao,Lei Ye,Yun Xu,Xiaolei ZhuPubMed 37192007
- NPJ Parkinson's disease 9:922023Synaptic mechanisms underlying onset and progression of memory deficits caused by hippocampal and midbrain synucleinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAttilio Iemolo,Maria De Risi,Nadia Giordano,Giulia Torromino,Cristina Somma,Diletta Cavezza,Martina Colucci,Maria Mancini,Antonio de Iure,Rocco Granata,Barbara Picconi,Paolo Calabresi,Elvira De LeonibusPubMed 37328503
- Cells 12:2023Cognitive Impairment Is Associated with AMPAR Glutamatergic Dysfunction in a Mouse Model of Neuronal Methionine Synthase Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiad Hassan,David Coelho,Carine Bossenmeyer-Pourié,Karim Matmat,Carole Arnold,Aurélie Savladori,Jean-Marc Alberto,Rémy Umoret,Jean-Louis Guéant,Grégory PouriéPubMed 37174668
- Molecular neurobiology 60:3379-33952023Caveolin-1-Mediated Cholesterol Accumulation Contributes to Exaggerated mGluR-Dependent Long-Term Depression and Impaired Cognition in Fmr1 Knockout Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Luo,Le Yang,Kun Zhang,Shi-Meng Zhou,Yan Wang,Liu-Kun Yang,Bin Feng,Shui-Bing Liu,Yu-Mei Wu,Ming-Gao Zhao,Qi YangPubMed 36854997
- Frontiers in molecular neuroscience 16:11073552023Neural mechanism underlies CYLD modulation of morphology and synaptic function of medium spiny neurons in dorsolateral striatum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Yi Tan,Jin-Xiang Jiang,Hui-Xian Huang,Xiu-Ping Mo,Jing-Ru Feng,Yu Chen,Li Yang,Cheng LongPubMed 36846565
- Dementia and geriatric cognitive disorders 51:434-4402022Exercise by Shaking Alleviates the Decline in Memory due to Aging: A Study in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSho Izawa,Kouji Yamada,Runhong Yao,Naoki Aizu,Takumi Kito,Daiki Iwata,Takeshi Chihara,Hirohide Sawada,Kazuhiro NishiiPubMed 36516802
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | IHC-FoFr | |
|---|---|---|---|
| Human | Predicted | Predicted | Predicted |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
| Common marmoset | Predicted | Predicted | Expected |
| Monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Monkey, Common marmoset | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Monkey, Common marmoset | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Common marmoset | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).
Alternative names
GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1
Recommended products
Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) is a rabbit polyclonal antibody that is used to detect Glutamate Receptor 1 (AMPA subtype) in Western Blot, IHC-P. Suitable for Common marmoset, Mouse, Rat samples.
- Over 160 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Based on sequence analysis, ab31232 shares 100% homology with Human, Mouse and Rat Glutamate Receptor 1 (AMPA subtype), however we have been unable to demonstrate reactivity with human tissues in experiments. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
Replenishment batches of our polyclonal antibody, ab31232 are tested in WB. Previous batches were additionally validated in IHC-FoFr and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab183797.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Common marmoset, Mouse, Rat samples.
Trusted by the scientific community
Anti-Glutamate Receptor 1 (AMPA subtype) (ab31232) was first used in a scientific publication in 2006 and has been cited over 160 times in peer-reviewed journals.
Reviewed by scientists
Anti-Glutamate Receptor 1 (AMPA subtype) (ab31232) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.
- Biological function summary
The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.
- Pathways
The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.
- Associated diseases and disorders
Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31232 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) at 1 µg/mL
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Brain (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa, 47 kDa
Exposure time: 20min
This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232)
Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) with ab31232 on formaldehyde-fixed paraffin-embedded mouse brain sections. Antigen retrieval step: heat mediated in Citric acid pH6 buffer.Blocking step: 1%BSA for 10 mins @ rt°C. Primary antibody ab31232 incubated at 1/100 for 2 hours. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows immunostaining in cell bodies and processes of a subset of neurones in the mouse cortex (upper image) and also in the dendritic fields of the molecular layer of the mouse cerebellum (lower image). Purkinje cells of the cerebellum are negative but their primary processes are clearly delineated by a punctate membrane-region positivity. What appears to be small cells in close contact with the Purkinje cell bodies are strongly positive (perhaps these are basket cells?).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232)
IHC image of Glutamate Receptor 1 (AMPA subtype) staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab31232, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232)
Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) with ab31232 on formaldehyde-fixed paraffin-embedded rat cerebellum sections. Antigen retrieval step: heat mediated in citric acid pH6. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody was incubated at 1/200 for 2 hours @ room temp. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows localisation of this protein apparently exclusive to the molecular layer of the cerebellum upper image (low power). In the lower image (higher power), the molecular layer positivity appears to be compartmentalised/restricted.
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