AB32436

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.

Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.

Image from van Vuurden DG et al., PLoS One. 2009 Jun 18;4(6):e5953. Fig 2.; doi:10.1371/journal.pone.0005953; June 18, 2009, PLoS ONE 4(6): e5953.

Lab

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

Purified ab32436 at 1/50 dilution (2μg) immunoprecipitating Glutamate Receptor 1 (AMPA subtype) in SH-SY5Y whole cell lysate.
Lane 1 (input) : SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab32436 + SH-SY5Y whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32436 in SH-SY5Y whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 106 kDa

All lanes:

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

Predicted band size: 102 kDa

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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Predicted band size: 102 kDa

Observed band size: 106 kDa

false

Carrier free

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E308

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for Glutamate Receptor 1 (AMPA subtype).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.

Biological function summary

The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.

Pathways

The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.

Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 1311100, PubMed : 20805473, PubMed : 21172611, PubMed : 28628100, PubMed : 35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed : 21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed : 21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).

See full target information GRIA1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:971385 PubMed36249769

2022

Sagacious confucius' pillow elixir ameliorates Dgalactose induced cognitive injury in mice estrogenic effects and synaptic plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

De-Ping Zhao,Xia Lei,Yue-Ying Wang,Ao Xue,Chen-Yu Zhao,Yan-Ming Xu,Yue Zhang,Guo-Liang Liu,Fang Geng,Hong-Dan Xu,Ning Zhang

The Journal of pathology 250:67-78 PubMed31579943

2019

Dysregulation of AMPA receptor subunit expression in sporadic ALS post-mortem brain.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna M Gregory,Matthew R Livesey,Karina McDade,Bhuvaneish T Selvaraj,Samantha K Barton,Siddharthan Chandran,Colin Smith

Experimental neurology 314:58-66 PubMed30660616

2019

Opioid receptors inhibit the spinal AMPA receptor Ca permeability that mediates latent pain sensitization.

Applications

Unspecified application

Species

Unspecified reactive species

Bradley K Taylor,Ghanshyam P Sinha,Renee R Donahue,Carolyn M Grachen,Jose A Morón,Suzanne Doolen

EBioMedicine 7:191-204 PubMed27322472

2016

Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Emanuele,Alessandro Esposito,Serena Camerini,Flavia Antonucci,Silvia Ferrara,Silvia Seghezza,Tiziano Catelani,Marco Crescenzi,Roberto Marotta,Claudio Canale,Michela Matteoli,Elisabetta Menna,Evelina Chieregatti

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:13505-15 PubMed25274827

2014

GluA1 phosphorylation contributes to postsynaptic amplification of neuropathic pain in the insular cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Qiu,Ming Zhang,Yan Liu,Yanyan Guo,Huan Zhao,Qian Song,Minggao Zhao,Richard L Huganir,Jianhong Luo,Hui Xu,Min Zhuo

Neuroscience letters 557 Pt B:101-6 PubMed24262606

2013

An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure.

Applications

Species

Rat

Ainhoa Plaza-Zabala,Xuan Li,Mike Milovanovic,Jessica A Loweth,Rafael Maldonado,Fernando Berrendero,Marina E Wolf

PloS one 4:e5953 PubMed19536293

2009

Attenuated AMPA receptor expression allows glioblastoma cell survival in glutamate-rich environment.

Applications

IHC-P

Species

Human

Dannis G van Vuurden,Maryam Yazdani,Ingeborg Bosma,Aart J F Broekhuizen,Tjeerd J Postma,Jan J Heimans,Paul van der Valk,Eleonora Aronica,Bakhos A Tannous,Thomas Würdinger,Gertjan J L Kaspers,Jacqueline Cloos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise