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Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436), expandable thumbnail
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436), expandable thumbnail
  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (AB32436), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).

Alternative names

GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1

Recommended products

Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
E308
Purification technique
Affinity purification Protein A
Specificity

This antibody is specific for Glutamate Receptor 1 (AMPA subtype).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.

Biological function summary

The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.

Pathways

The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.

Associated diseases and disorders

Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436), expandable thumbnail
    Image from van Vuurden DG et al., PLoS One. 2009 Jun 18;4(6):e5953. Fig 2.; doi:10.1371/journal.pone.0005953; June 18, 2009, PLoS ONE 4(6): e5953.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.


    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436), expandable thumbnail

    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution

    All lanes: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Predicted band size: 102 kDa

    Observed band size: 106 kDa

  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436), expandable thumbnail

    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    Purified ab32436 at 1/50 dilution (2μg) immunoprecipitating Glutamate Receptor 1 (AMPA subtype) in SH-SY5Y whole cell lysate.
    Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab32436 + SH-SY5Y whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32436 in SH-SY5Y whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 106 kDa

    All lanes: Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    Predicted band size: 102 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.

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Product protocols

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