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Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 58 publications.


Images

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450), expandable thumbnail
  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450), expandable thumbnail
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450), expandable thumbnail
  • OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450), expandable thumbnail
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/10.00000 - 1/100.00000
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

For unpurified use at 1/10000 - 1/50000.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.

Species
Rat
Dilution info
1/2000
Notes

For unpurified use at 1/10000 - 1/50000.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.

Species
Human
Dilution info
1/2000
Notes

For unpurified use at 1/10000 - 1/50000.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).

Alternative names

GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1

Recommended products

Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 58 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5479
Purification technique
Affinity purification Protein A
Dissociation constant
1.94 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.

Biological function summary

The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.

Pathways

The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.

Associated diseases and disorders

Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450), expandable thumbnail

    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    Exposure times:

    Lanes 1 and 2: 180 seconds
    Lanes 3 and 4: 50 seconds
    Lanes 5 and 6: 60 seconds

    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution

    Lane 1: Human brain lysates prepared in RIPA lysis method at 20 µg

    Lane 2: Human brain lysates prepared in 1%SDS Hot lysis method at 20 µg

    Lane 3: Rat brain lysates prepared in RIPA lysis method at 20 µg

    Lane 4: Rat brain lysates prepared in 1%SDS Hot lysis method at 20 µg

    Lane 5: Mouse brain lysates prepared in RIPA lysis method at 20 µg

    Lane 6: Mouse brain lysates prepared in 1%SDS Hot lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 102 kDa

    Observed band size: 102 kDa

  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450), expandable thumbnail

    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    Predicted band size: 102 kDa

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450), expandable thumbnail

    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution

    Lane 1: Human brain lysate at 10 µg

    Lane 2: Mouse brain lysate at 10 µg

    Lane 3: Rat brain lysate at 10 µg

    Predicted band size: 102 kDa

    Observed band size: 106 kDa

  • OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450), expandable thumbnail

    OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    Glutamate Receptor 1 (AMPA subtype) western blot using anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450. Publication image and figure legend from Luo, Y. X., Xue, Y. X., et al., 2015, Nat Commun, PubMed 26169171.


    ab109450 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109450 please see the product overview.

    Exposure to UCS decreased membrane expression of GluA1 and GluA2 in BLA.(a) Timeline of the experimental procedure. (b) Membrane expression of GluA1 and GluA2 proteins after the different experiment manipulations. Values are the mean percentage of protein levels in the BLA in reference to the saline+1 h+extinction group. One-way ANOVA, *Different from group ‘saline+1 h+extinction', *P<0.05; n=6 per experimental condition. Error bars represent s.e.m. Uncropped western blots of the data depicted in Fig. 5 are provided in Supplementary Fig. 10.

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