Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Images
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (AB208789), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-epr5479-bsa-and-azide-free-ab208789--western-blot-img142928.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (AB208789)")
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (AB208789), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-epr5479-bsa-and-azide-free-ab208789--immunoprecipitation-img492.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (AB208789)")
![OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (AB208789), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-epr5479-bsa-and-azide-free-ab208789--oi-rd-scanning-img139474.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (AB208789)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).
Alternative names
GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1
Recommended products
Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5479
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.94 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab208789 is the carrier-free version of Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.
- Biological function summary
The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.
- Pathways
The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.
- Associated diseases and disorders
Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-epr5479-bsa-and-azide-free-ab208789--western-blot-img142928.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)")
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
This WB data was generated using the same anti-Gluatamate Receptor 1 (AMPA subtype) antibody clone, EPR5479, in a different buffer formulation (cat# Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789) at 1/2000 dilution
All lanes: Human brain lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 102 kDa
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-epr5479-bsa-and-azide-free-ab208789--immunoprecipitation-img492.jpg "Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)")
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450).
All lanes: Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450)
Predicted band size: 102 kDa
![OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-epr5479-bsa-and-azide-free-ab208789--oi-rd-scanning-img139474.jpg "OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)")
OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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