AB174785

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal

Name: Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) | Abcam
Brand: Abcam Limited
SKU: AB174785
Price: 215 USD
Availability: InStock
Rating: 3 (1 reviews)

BOND RX™ Validated
Recombinant
What is this?

(1 Review)

(11 Publications)

Mouse Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Gria1 aa 1-400.

View Alternative Names

GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus labeling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. Anti-MAP2 antibody [EPR19691] (ab183830) was used as a counterstain at 1/1000 dilution and was co-incubated with ab174785 overnight at 4° C, followed by Alexa Fluor^® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus labeling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat primary neuron cells labelling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. Anti-MAP2 antibody [EPR19691] (ab183830) was used as a counterstain at 1/1000 dilution and was co-incubated with ab174785 overnight at 4° C, followed by Alexa Fluor^® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

Lab

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Lane 5:

Rat hippocampus tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N355/1

Isotype

IgG1

Carrier free

Reacts with

Rat, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Gria1 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P19490

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 1st November 2024.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.

Biological function summary

The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.

Pathways

The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.

Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 1311100, PubMed : 20805473, PubMed : 21172611, PubMed : 28628100, PubMed : 35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed : 21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed : 21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).

See full target information GRIA1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

The Journal of endocrinology 260: PubMed37991884

2023

Testosterone reduces hippocampal synaptic damage in an androgen receptor-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Zhang,Meiqin Chen,Huan Chen,Shixiong Mi,Chang Wang,Hongchun Zuo,Leigang Song,Juan Du,Huixian Cui,Sha Li

Cell reports methods 3:100408 PubMed36936077

2023

Quantitative DNA-PAINT imaging of AMPA receptors in live neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yeoan Youn,Gloria W Lau,Yongjae Lee,Barun Kumar Maity,Eric Gouaux,Hee Jung Chung,Paul R Selvin

iScience 26:105732 PubMed36590162

2023

Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea M Reyes-Ortiz,Edsel M Abud,Mara S Burns,Jie Wu,Sarah J Hernandez,Nicolette McClure,Keona Q Wang,Corey J Schulz,Ricardo Miramontes,Alice Lau,Neethu Michael,Emily Miyoshi,David Van Vactor,John C Reidling,Mathew Blurton-Jones,Vivek Swarup,Wayne W Poon,Ryan G Lim,Leslie M Thompson

Neural regeneration research 16:319-324 PubMed32859791

2020

Constraint-induced movement therapy enhances AMPA receptor-dependent synaptic plasticity in the ipsilateral hemisphere following ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hu,Pei-Le Liu,Yan Hua,Bei-Yao Gao,Yu-Yuan Wang,Yu-Long Bai,Chan Chen

Nature communications 11:3688 PubMed32703948

2020

Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey Bingor,Tomer Haham,Claire Thornton,Yael Stern-Bach,Rami Yaka

Brain research bulletin 160:8-23 PubMed32298779

2020

Constraint-induced movement therapy improves functional recovery after ischemic stroke and its impacts on synaptic plasticity in sensorimotor cortex and hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hu,Ce Li,Yan Hua,Peile Liu,Beiyao Gao,Yuyuan Wang,Yulong Bai

Molecular psychiatry 25:3360-3379 PubMed31636381

2019

UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Notaras,Megan Allen,Francesco Longo,Nicole Volk,Miklos Toth,Noo Li Jeon,Eric Klann,Dilek Colak

Biochemical and biophysical research communication 516:1204-1210 PubMed31296385

2019

βPix heterozygous mice have defects in neuronal morphology and social interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Younghee Kwon,Seung Joon Lee,Eunee Lee,Daesoo Kim,Dongeun Park

PloS one 12:e0177156 PubMed28545069

2017

Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Kaveri Arora,Jeffrey M Sequeira,Alejandro I Hernández,Juan M Alarcon,Edward V Quadros

Molecular biology of the cell 28:285-295 PubMed27852895

2016

Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover.

Applications

Species

Mouse

Zsófia Szíber,Hanna Liliom,Carlos O Oueslati Morales,Attila Ignácz,Anikó Erika Rátkai,Kornelia Ellwanger,Gisela Link,Attila Szűcs,Angelika Hausser,Katalin Schlett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise