Mouse Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Gria1 aa 1-400.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441554.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunocytochemistry-immunofluorescence-img441555.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)")
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--western-blot-img441552.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunocytochemistry-immunofluorescence-img441556.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441553.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (AB174785)")
Publications
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- The Journal of endocrinology 260:2023Testosterone reduces hippocampal synaptic damage in an androgen receptor-independent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizhou Zhang,Meiqin Chen,Huan Chen,Shixiong Mi,Chang Wang,Hongchun Zuo,Leigang Song,Juan Du,Huixian Cui,Sha LiPubMed 37991884
- Cell reports methods 3:1004082023Quantitative DNA-PAINT imaging of AMPA receptors in live neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeoan Youn,Gloria W Lau,Yongjae Lee,Barun Kumar Maity,Eric Gouaux,Hee Jung Chung,Paul R SelvinPubMed 36936077
- iScience 26:1057322023Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea M Reyes-Ortiz,Edsel M Abud,Mara S Burns,Jie Wu,Sarah J Hernandez,Nicolette McClure,Keona Q Wang,Corey J Schulz,Ricardo Miramontes,Alice Lau,Neethu Michael,Emily Miyoshi,David Van Vactor,John C Reidling,Mathew Blurton-Jones,Vivek Swarup,Wayne W Poon,Ryan G Lim,Leslie M ThompsonPubMed 36590162
- Neural regeneration research 16:319-3242020Constraint-induced movement therapy enhances AMPA receptor-dependent synaptic plasticity in the ipsilateral hemisphere following ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Hu,Pei-Le Liu,Yan Hua,Bei-Yao Gao,Yu-Yuan Wang,Yu-Long Bai,Chan ChenPubMed 32859791
- Nature communications 11:36882020Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexey Bingor et. al.PubMed 32703948
- Brain research bulletin 160:8-232020Constraint-induced movement therapy improves functional recovery after ischemic stroke and its impacts on synaptic plasticity in sensorimotor cortex and hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Hu et. al.PubMed 32298779
- Molecular psychiatry 25:3360-33792019UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Notaras,Megan Allen,Francesco Longo,Nicole Volk,Miklos Toth,Noo Li Jeon,Eric Klann,Dilek ColakPubMed 31636381
- Biochemical and biophysical research communication 516:1204-12102019βPix heterozygous mice have defects in neuronal morphology and social interaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYounghee Kwon et. al.PubMed 31296385
- PloS one 12:e01771562017Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaveri Arora et. al.PubMed 28545069
- Molecular biology of the cell 28:285-2952016Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover.Applications:WBReactive species:MouseZsófia Szíber,Hanna Liliom,Carlos O Oueslati Morales,Attila Ignácz,Anikó Erika Rátkai,Kornelia Ellwanger,Gisela Link,Attila Szűcs,Angelika Hausser,Katalin SchlettPubMed 27852895
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Rat Gria1 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link P19490
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
| Rat | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 | Notes - |
Species Rat | Dilution info 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes - |
Species Rat | Dilution info 1/200 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).
Alternative names
GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1
Recommended products
Mouse Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Gria1 aa 1-400.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Rat Gria1 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link P19490
- Clone number
- N355/1
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product has switched from a hybridoma to recombinant production method on 1st November 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.
- Biological function summary
The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.
- Pathways
The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.
- Associated diseases and disorders
Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441554.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Immunohistochemical analysis of paraffin-embedded Rat hippocampus labeling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunocytochemistry-immunofluorescence-img441555.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)")
Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution at RT for 45 min. Anti-MAP2 antibody [EPR19691] (Anti-MAP2 antibody [EPR19691] ab183830) was used as a counterstain at 1/1000 dilution and was co-incubated with ab174785 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--western-blot-img441552.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)")
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat cerebellum tissue lysate at 20 µg
Lane 5: Rat hippocampus tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
![Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunocytochemistry-immunofluorescence-img441556.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)")
Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat primary neuron cells labelling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution at RT for 45 min. Anti-MAP2 antibody [EPR19691] (Anti-MAP2 antibody [EPR19691] ab183830) was used as a counterstain at 1/1000 dilution and was co-incubated with ab174785 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-ab174785--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441553.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus labeling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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