Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) phospho S831 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 31 publications.
Images
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img46183.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464)")
![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--dot-blot-img18266.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464)")
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img46188.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464)")
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img24352.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464)")
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img443190.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (AB109464)")
Publications
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- Molecular pain 19:174480692312224062023A-kinase anchoring protein 79/150 coordinates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor sensitization in sensory neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhang,Nathaniel A JeskePubMed 38073552
- Evidence-based complementary and alternative medicine : eCAM 2023:60355892023The Effects of Ninjinyoeito on Impaired Spatial Memory and Prefrontal Cortical Synaptic Plasticity through -Amino-3-hydroxy-5-4-isoxazole Propionic Acid Receptor Subunit in a Rat Model with Cerebral Ischemia and -Amyloid Injection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaki Nagao,Akinobu Hatae,Kazuma Mine,Soichiro Tsutsumi,Hiroya Omori,Marika Hirata,Maaya Arimatsu,Chise Taniguchi,Takuya Watanabe,Kaori Kubota,Shutaro Katsurabayashi,Katsunori IwasakiPubMed 37808130
- Scientific reports 13:139272023PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuk-Shin Lee,Tae-Hyun Kim,Hana Park,Ji-Eun KimPubMed 37626185
- International journal of molecular sciences 24:2023Functional and Molecular Changes in the Prefrontal Cortex of the Chronic Mild Stress Rat Model of Depression and Modulation by Acute Ketamine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Mingardi,Elona Ndoj,Tiziana Bonifacino,Paulina Misztak,Matteo Bertoli,Luca La Via,Carola Torazza,Isabella Russo,Marco Milanese,Giambattista Bonanno,Maurizio Popoli,Alessandro Barbon,Laura MusazziPubMed 37445990
- Molecular pain 19:174480692311776342023Sophoridine alleviates hyperalgesia and anxiety-like behavior in an inflammatory pain mouse model induced by complete freund's adjuvant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Rong,Le Yang,Yue Chen,Yan Qin,Cai-Yan Cheng,Jun Zhao,Long-Fei Li,Xue Ma,Yu-Mei Wu,Shui-Bing Liu,Yan-Ni Liang,Ming-Gao ZhaoPubMed 37207346
- International journal of molecular sciences 24:2023Dopamine-Dependent Ketamine Modulation of Glutamatergic Synaptic Plasticity in the Prelimbic Cortex of Adult Rats Exposed to Acute Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLia Forti,Elona Ndoj,Jessica Mingardi,Emanuele Secchi,Tiziana Bonifacino,Emanuele Schiavon,Giulia Carini,Luca La Via,Isabella Russo,Marco Milanese,Massimo Gennarelli,Giambattista Bonanno,Maurizio Popoli,Alessandro Barbon,Laura MusazziPubMed 37240064
- Frontiers in cellular neuroscience 17:11176972023Epileptiform activity influences theta-burst induced LTP in the adult hippocampus: a role for synaptic lipid raft disruption in early metaplasticity?Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé D Carvalho-Rosa,Nádia C Rodrigues,Armando Silva-Cruz,Sandra H Vaz,Diana Cunha-ReisPubMed 37228704
- Brain sciences 12:2022The Effects of Intraoperative Hypothermia on Postoperative Cognitive Function in the Rat Hippocampus and Its Possible Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangyan Xu,Tianjia Li,Yuguang HuangPubMed 35053838
- The Journal of biological chemistry 297:1010342021Acetylation of calmodulin regulates synaptic plasticity and fear learning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Long Zhang,Bing Zhao,Wei Han,Yi-Bei Sun,Pin Yang,Yongjun Chen,Duan Ni,Jian Zhang,Dong-Min YinPubMed 34339735
- Neural plasticity 2020:88619942020Electroacupuncture Attenuates CFA-Induced Inflammatory Pain by Regulating CaMKII.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixiao Gu et. al.PubMed 33488694
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | Dot | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Expected | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Expected | Tested | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity).
Alternative names
GLUA1, GLUH1, GLUR1, GRIA1, Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1
Recommended products
Rabbit Recombinant Monoclonal Glutamate Receptor 1 (AMPA subtype) phospho S831 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 31 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1887
- Purification technique
- Affinity purification Protein A
- Specificity
ab109464 only detects Glutamate Receptor 1 phosphorylated at Serine 831.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamate Receptor 1 (AMPA subtype) also known as GluR1 is a subunit of the AMPA receptor complex which mediates fast synaptic transmission in the central nervous system. It is an ionotropic receptor for glutamate functioning by opening ion channels to allow the flow of Na+ and Ca2+ ions across the cell membrane contributing to excitatory neurotransmission. The GluR1 subunit has a molecular mass of approximately 100 kDa. This receptor is commonly expressed in the brain regions such as the hippocampus and the cerebral cortex playing an important role in synaptic plasticity and memory formation.
- Biological function summary
The GluR1 subunit is an essential component of the AMPA receptor complex which typically forms as a tetramer. This complex modulates synaptic strength and plasticity processes critical for learning and memory. The activity of AMPA receptors including those containing GluR1 is regulated by several auxiliary proteins and is essential for post-synaptic responses. The GluR1 subunit also interacts with other proteins such as TARPs which modulate its trafficking and channel properties.
- Pathways
The GluR1-containing AMPA receptors participate significantly in the glutamatergic signaling pathway which is vital for fast excitatory synaptic transmission in the brain. This pathway also involves the NMDA receptors which work together with AMPA receptors to regulate synaptic plasticity and neuronal communication. Additionally the GluR1 interacts within the long-term potentiation (LTP) pathway contributing to the strengthening of synapses an essential mechanism underlying learning and memory.
- Associated diseases and disorders
Dysfunction in GluR1 and associated AMPA receptors has been implicated in conditions like Alzheimer's disease and epilepsy. Alzheimer's disease exhibits decreased synaptic transmission and plasticity linked to impaired GluR1 function and its interactions with NMDA receptors. In epilepsy abnormal GluR1 activity may contribute to heightened neuronal excitability and seizure propagation. Targeting GluR1 or associated pathways offers potential for therapeutic interventions in these disorders possibly through drugs such as memantine and NBQX which modulate receptor activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img46183.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
Blocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1: Mouse hippocampus tissue lysate at 15 µg
Lane 2: Mouse hippocampus tissue lysate, membrane incubated with phosphatase at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa
Exposure time: 5s
![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--dot-blot-img18266.jpg "Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) with ab109464 at 1/1000 exposed for 3 minutes. Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) Goat Anti-Rabbit IgG H&L (HRP) ab97051 (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.
Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS831) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img46188.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
Blocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1: Human cerebellum whole cell lysates at 15 µg
Lane 2: Human cerebellum whole cell lysates, the membrane was incubated with phosphatase. at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa
Exposure time: 10s
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img24352.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation.
All lanes: Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1: Rat brain lysates, untreated at 10 µg
Lane 2: Rat brain lysates treated with Lambda Phosphatase at 10 µg
SecondaryAll lanes: HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464), expandable thumbnail](https://content.abcam.com/products/images/glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-ab109464--western-blot-img443190.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
Glutamate Receptor 1 (AMPA subtype) (phospho S831) western blot using anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] ab109464. Publication image and figure legend from Kim, J. E., Choi, H. C., et al., 2019, Front Cell Neurosci, PubMed 31118889.
ab109464 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109464 please see the product overview.
Effects of MK801, perampanel (PER) and GYKI 52466 on GluR1 expression and its phosphorylations. MK801 does not affect GluR1 expression and its phosphorylations. Perampanel and GYKI 52466 reduce GluR1 expression, but increase GluR1-S831, not -S845, phosphorylation. (A) Representative images for western blot of GluR1, phospho (p)-GluR1-S831 and pGluR1-S845 levels in the hippocampal tissues. (B–D) Quantifications of GluR1, pGluR1-S831, and pGluR1-S845 levels. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM (∗p < 0.05 vs. vehicle; n = 7, respectively).
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