Anti-Glutamate receptor 3/GluA3 antibody [EP813Y]

• WB

Lab

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (AB40845)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution

All lanes:

Human hippocampus lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 101 kDa

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• WB

Unknown

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (AB40845)

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution

All lanes:

human brain lysate at 10 µg

Predicted band size: 101 kDa

Observed band size: 95 kDa

false

• WB

Lab

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (AB40845)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/2000 dilution

Lane 1:

Mouse hippocampus lysates at 20 µg

Lane 2:

Rat brain lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 101 kDa

Observed band size: 100 kDa

false

• WB

Lab

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (AB40845)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

In Western blot, Anti-Myc tag antibody [9E10] - Loading Control (ab32) staining at 1/10000 dilution.

This antibody does not cross-react with Human GRIA1,GRIA2 and GRIA4.

Exposure time : ab40845 : 40s, ab32 : 80s.

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution

Lane 1:

293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with Human fragment GRIA2 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with Human full length GRIA3 expression vector containing a n-term myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with human full length GRIA4 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

Recombinant Human full length GRIA1 Protein containing a C-Myc/DDK tag at 100 ng

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 1 - 5:

Goat anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution

Observed band size: 100 kDa

false

• WB

CiteAb

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (AB40845)

Glutamate receptor 3/GluA3 western blot using anti-Glutamate receptor 3/GluA3 antibody [EP813Y] ab40845. Publication image and figure legend from Adotevi, N. K. & Leitch, B., 2018, Front Mol Neurosci, PubMed 29311821.

ab40845 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab40845 please see the product overview.

Relative synaptic expression of GluA1–4 in the somatosensory cortex. (A–D) Representative immunoblots showing extrasynaptic and synaptic AMPAR subunit expression; (A) GluA1, (B) GluA2, (C) GluA3 and (D) GluA4, with the loading markers PSD95 and Pan-cadherin. (E) Scatter-plot with bar graphs showing a significant decrease in AMPAR GluA1 (0.79 ± 0.07, n = 6, p < 0.05), GluA2 (0.77 ± 0.08, n = 5, p < 0.05), GluA3 (0.77 ± 0.06, n = 7, p < 0.05), GluA4 (0.66 ± 0.02, n = 5, p < 0.01) expression in stargazer (E) synaptic fractions relative to NE control littermates after normalization against Pan-cadherin. Symbols '*' indicate p value significant digits (*p < 0.05, ***p < 0.005).

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