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AB232430

Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Glutamate receptor 3/GluA3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

GluA3, GLUR3, GLURC, GRIA3, Glutamate receptor 3, GluR-3, AMPA-selective glutamate receptor 3, GluR-C, GluR-K3

1 Images
Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (AB232430)
  • WB

Supplier Data

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (AB232430)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40845).

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430) at 1/5000 dilution

All lanes:

Human brain lysate at 10 µg

Predicted band size: 101 kDa

Observed band size: 95 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP813Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab232430 is the carrier-free version of ab40845.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate receptor 3 also known as GluA3 or GRIA3 is an ionotropic receptor that mediates fast excitatory synaptic transmission in the central nervous system. It is part of the AMPA receptor family and has a molecular mass of approximately 100 kDa. This receptor predominantly localizes in the brain where it plays a significant role in the mediation of glutamate neurotransmission influencing synaptic plasticity and communication among neurons. Its expression is vital for transmitting signals across excitatory synapses affecting learning and memory processes.
Biological function summary

GluA3 operates as a subunit of AMPA receptors forming tetrameric complexes that facilitate synaptic signal transmission. These channels permit the influx of sodium and to a lesser extent calcium ions when activated by the neurotransmitter glutamate. The interaction of GluA3 with auxiliary proteins like transmembrane AMPA receptor regulatory proteins (TARPs) can modulate its function and trafficking. As an essential component of synaptic transmission GluA3 influences synaptic strength and is involved in various neuronal activities.

Pathways

GluA3 plays an important role in synaptic plasticity and long-term potentiation (LTP). Two important pathways associated with GluA3 are the mitogen-activated protein kinase (MAPK) pathway and the calcium/calmodulin-dependent protein kinase II (CaMKII) pathway. The regulation of these pathways through GluA3 modulation affects synaptic efficacy and memory formation. GluA3 interacts with proteins like PSD-95 which influences receptor assembly and synapse structure integrating into broader signaling networks that govern cellular responses and adaptations.

GluA3 has been linked to neurodegenerative diseases and psychiatric disorders. Alterations in GluA3 expression or function are associated with Alzheimer's disease where disrupted glutamatergic signaling contributes to cognitive decline. Additionally GluA3 plays a role in schizophrenia where AMPA receptor dysfunction affects neural circuitry and synaptic plasticity. In these contexts GluA3 often interacts with proteins like NMDA receptors where their combined dysfunction can lead to imbalances in excitatory-inhibitory signaling exacerbating disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission by inducing long-term potentiation (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of calcium (PubMed : 17989220). The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 17989220). In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate (PubMed : 21172611).
See full target information GRIA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 12:395-425 PubMed33676046

2021

Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Yuan Mao,Yi-Pin Lv,Chuan-Jie Hao,Yong-Sheng Teng,Yu-Gang Liu,Ping Cheng,Shi-Ming Yang,Weisan Chen,Tao Liu,Quan-Ming Zou,Rui Xie,Jing-Yu Xu,Yuan Zhuang
View all publications
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Product promise

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