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Rabbit Recombinant Monoclonal GluA4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (AB280199), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (AB280199), expandable thumbnail
  • Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (AB280199), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (AB280199), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (AB280199), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611).

Alternative names

GluA4, GLUR4, GRIA4, Glutamate receptor 4, GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D

Recommended products

Rabbit Recombinant Monoclonal GluA4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24283-69
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutamate receptor 4 also known as GluR4 or AMPA receptor 4 is an ionotropic glutamate receptor subunit. This protein has approximately 100 kDa molecular weight. Structurally GluR4 functions as a ligand-gated ion channel that selectively conducts sodium and potassium ions across the neuronal cell membrane. It is typically expressed in the central nervous system with a higher concentration in cerebellar Purkinje cells hippocampal pyramidal neurons and other areas involved in synaptic transmission. This receptor plays a fundamental role in mediating fast excitatory synaptic transmission.

Biological function summary

GluR4 facilitates the fast excitatory neurotransmission by binding to the neurotransmitter glutamate. It forms part of the AMPA receptor complex which consists of four subunits that assemble to create a functional receptor. By enabling rapid and precise control over synaptic signaling GluR4 participates importantly in processes like synaptic plasticity a cellular mechanism for learning and memory. The receptor's dynamic regulation of ion flow into the neuron underpins its role in shaping the physiological response to excitatory stimuli.

Pathways

GluR4 plays an important role within the glutamatergic synapse pathway. It interacts directly with other proteins such as stargazin which assists in the trafficking and anchoring of AMPA receptors at synaptic sites. GluR4 is also involved in the long-term potentiation pathway a mechanism for strengthening synapses that is vital for learning. When glutamate opens the channel GluR4 allows the influx of sodium ions which can lead to depolarization and trigger downstream signaling events. This receptor works in conjunction with NMDA receptors to modulate synaptic strength and connectivity.

Associated diseases and disorders

GluR4 has been implicated in neurological conditions such as epilepsy and neurodegenerative diseases like Alzheimer's. In epilepsy dysregulation of glutamate receptors including GluR4 can lead to abnormal neuronal excitability and seizures. Another connection exists with amyloid beta protein found in Alzheimer's disease which can disrupt glutamate receptor function leading to synaptic dysfunction. Investigating these associations helps to understand the pathological mechanisms and potential therapeutic approaches targeting GluR4-related pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199), expandable thumbnail

    Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)

    Negative control: human liver (PMID:1372042)

    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 101 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199), expandable thumbnail

    Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)

    Negative control: liver (PMID:1372042)

    Exposure time: 7.75 seconds

    All lanes: Western blot - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution

    Lane 1: Rat cerebellum tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Lane 3: Rat liver tissue lysate at 40 µg

    Lane 4: Mouse cerebellum tissue lysate at 20 µg

    Lane 5: Mouse brain tissue lysate at 20 µg

    Lane 6: Mouse liver tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 101 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum. (PMID: 20662939) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse liver. (PMID: 1372042) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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