AB106289

Anti-Glutamate Transporter 1 antibody

Name: Anti-Glutamate Transporter 1 antibody (ab106289) | Abcam
Brand: Abcam Limited
SKU: AB106289
Price: 535 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(11 Publications)

Rabbit Polyclonal EAAT2 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc1a2.

View Alternative Names

Eaat2, Glt1, Slc1a2, Excitatory amino acid transporter 2, GLT-1, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2, GLUT-R

1 Images

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Western blot - Anti-Glutamate Transporter 1 antibody (AB106289)

Lane 3 shows inhibition of the immunoreactivity of ab106289 with blocking peptide.
Glutamate Transporter 1 protein is detected at 35 kDa and 70 kDa as a dimer.

All lanes:

Western blot - Anti-Glutamate Transporter 1 antibody (ab106289) at 1/1000 dilution

Lane 1:

Protein extract from rat brain

Lane 2:

Protein extract from mouse brain

Predicted band size: 62 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

Synthetic Peptide within Rat Slc1a2. The exact immunogen used to generate this antibody is proprietary information.

P31596

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate Transporter 1 (GLT-1) also recognized as Excitatory Amino Acid Transporter 2 (EAAT2) is essential for regulating the levels of glutamate in the central nervous system. This protein features a mass of approximately 70 kDa and is largely expressed in astrocytes within the brain. It operates by transporting glutamate from the extracellular space into the cells utilizing the sodium concentration gradient as a driving force. This active transport mechanism prevents the accumulation of glutamate in the synaptic cleft reducing excitotoxicity which is harmful to neural cells.

Biological function summary

GLT-1 serves to maintain neurotransmitter homeostasis critical for proper synaptic functioning. It is a part of the larger SLC1 (solute carrier family 1) protein family which includes other transporters with similar functions. GLT-1 works closely with other glutamate transporters to help ensure synaptic signalings such as sensory perception and cognition proceed without interruption or damage. By efficiently clearing excessive glutamate from synapses it helps protect neurons from excitotoxic stress which can lead to cell death.

Pathways

This transporter is integral in the glutamatergic signaling pathway which plays an important role in synaptic plasticity and memory formation. GLT-1 closely interacts with proteins involved in metabolic pathways such as the glutamate-glutamine cycle ensuring that glutamate is recycled efficiently without causing cellular stress. It is also linked with EAAT1 another member of the excitatory amino acid transporters working in tandem to modulate the balance of neurotransmitter levels.

Mutations or dysfunctions of GLT-1 have been implicated in neurological conditions such as amyotrophic lateral sclerosis (ALS) and epilepsy. In ALS the downregulation of GLT-1 can lead to insufficient clearance of glutamate contributing to motor neuron degeneration. This protein is additionally associated with EAAT2 which is often studied for its role in preventing excitotoxic damage that might exacerbate these neurologic conditions. Researchers are exploring therapeutics like riluzole to modulate GLT-1 activity and alleviate symptoms related to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 1448170, PubMed : 7913472). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 1448170). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).

See full target information Slc1a2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36362260

2022

Delayed Impairment of Hippocampal Synaptic Plasticity after Pentylenetetrazole-Induced Seizures in Young Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana Y Postnikova,Alina M Trofimova,Maria V Zakharova,Olga I Nosova,Alexey R Brazhe,Dmitry E Korzhevskii,Alexey V Semyanov,Aleksey V Zaitsev

Nutrients 13: PubMed34836132

2021

Dose-Dependent Neuroprotective Effects of Bovine Lactoferrin Following Neonatal Hypoxia-Ischemia in the Immature Rat Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Sanches,Yohan van de Looij,Sadou Sow,Audrey Toulotte,Analina da Silva,Laura Modernell,Stéphane Sizonenko

The Journal of neuroscience : the official journal 40:9751-9771 PubMed33158962

2020

Astrocyte-Derived Estrogen Regulates Reactive Astrogliosis and is Neuroprotective following Ischemic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Gangadhara R Sareddy,Yujiao Lu,Uday P Pratap,Fulei Tang,Karah M Greene,Pornjittra L Meyre,Rajeshwar R Tekmal,Ratna K Vadlamudi,Darrell W Brann

The Journal of neuroscience : the official journal 40:7355-7374 PubMed32817249

2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yujiao Lu,Gangadhara R Sareddy,Jing Wang,Quanguang Zhang,Fu-Lei Tang,Uday P Pratap,Rajeshwar R Tekmal,Ratna K Vadlamudi,Darrell W Brann

Molecular medicine reports 21:1809-1818 PubMed32319622

2020

Ginkgo biloba extract protects diabetic rats against cerebral ischemia‑reperfusion injury by suppressing oxidative stress and upregulating the expression of glutamate transporter 1.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Yan,Mei Li,Shuling Gu,Zheng Sun,Tengfei Ma,Xing Ma

Journal of pain research 12:3067-3078 PubMed32009812

2019

Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wu,Yongliang Jiang,Xiaomei Shao,Xiaofen He,Zui Shen,Yan Shi,Chao Wang,Jianqiao Fang

Frontiers in cellular neuroscience 12:302 PubMed30386206

2018

A Purinergic P2 Receptor Family-Mediated Increase in Thrombospondin-1 Bolsters Synaptic Density and Epileptic Seizure Activity in the Amygdala-Kindling Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Hongliu Sun,Luyu Ma,Yurong Zhang,Xiaohong Pan,Chaoyun Wang,Jinjin Zhang,Xiuli Zhang,Hongwei Sun,Qiaoyun Wang,Wei Zhu

Frontiers in neurology 9:480 PubMed29988536

2018

Brain Metabolism Alterations Induced by Pregnancy Swimming Decreases Neurological Impairments Following Neonatal Hypoxia-Ischemia in Very Immature Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo F Sanches,Yohan Van de Looij,Audrey Toulotte,Analina R da Silva,Jacqueline Romero,Stephane V Sizonenko

Molecular medicine reports 16:1739-1746 PubMed28627708

2017

Alterations in AQP4 expression and polarization in the course of motor neuron degeneration in SOD1G93A mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Dai,Weihao Lin,Minying Zheng,Qiang Liu,Baixuan He,Chuanming Luo,Xilin Lu,Zhong Pei,Huanxing Su,Xiaoli Yao

Acta neuropathologica communications 5:21 PubMed28283027

2017

Integrated analysis of genetic, behavioral, and biochemical data implicates neural stem cell-induced changes in immunity, neurotransmission and mitochondrial function in Dementia with Lewy Body mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Lakatos,Natalie R S Goldberg,Mathew Blurton-Jones

View all publications

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Product promise

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Anti-Glutamate Transporter 1 antibody

Western blot - Anti-Glutamate Transporter 1 antibody (AB106289)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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