AB260047

Anti-Glutaminase antibody

Name: Anti-Glutaminase antibody (ab260047) | Abcam
Brand: Abcam Limited
SKU: AB260047

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(7 Publications)

Rabbit Polyclonal Glutaminase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLS aa 600 to C-terminus.

View Alternative Names

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry anlaysis of human skeletal muscle tissue labeling Glutaminase with ab260047 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry anlaysis of human duodenum tissue labeling Glutaminase with ab260047 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry anlaysis of human kidney tissue labeling Glutaminase with ab260047 at a 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody (AB260047)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody (AB260047)

PFA-fixed, Triton X-100 permeabilized SiHa (human cervix squamous cell carcinoma cell line) cells labeling Glutaminase using ab260047 at 4 μg/ml in ICC/IF (green). Microtubules are shown in red, nuclei in blue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry anlaysis of human cerebral cortex tissue labeling Glutaminase with ab260047 at a 1/200 dilution.

Supplier Data

Western blot - Anti-Glutaminase antibody (AB260047)

All lanes:

Western blot - Anti-Glutaminase antibody (ab260047) at 0.4 µg/mL

All lanes:

EFO-21 (human metastatic ovarian serous cystadenocarcinoma cell line) cell lysate

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GLS aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94925

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.

Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed : 30239721, PubMed : 30575854, PubMed : 30970188).. Isoform 2. Lacks catalytic activity.

See full target information GLS

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:34135 PubMed41034261

2025

Clinicopathological analysis of GLUT-1, GLS1, and GLS2 expression in breast cancer tissues from Jordanian patients.

Applications

Unspecified application

Species

Unspecified reactive species

Haneen A Basheer,Ahlam Zaid Alkilani,Samir Al Bashir,Maram A Alhusban,Lina Elsalem,Sura Bilal AlOmari,Qosay Mahmoud Sharqiah,Kamyar Afarinkia

Signal transduction and targeted therapy 10:27 PubMed39837870

2025

Identification of glutamine as a potential therapeutic target in dry eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoniao Chen,Chuyue Zhang,Fei Peng,Lingling Wu,Deyi Zhuo,Liqiang Wang,Min Zhang,Zhaohui Li,Lei Tian,Ying Jie,Yifei Huang,Xinji Yang,Xiaoqi Li,Fengyang Lei,Yu Cheng

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers

Journal of translational medicine 20:120 PubMed35272674

2022

RUNX3-mediated circDYRK1A inhibits glutamine metabolism in gastric cancer by up-regulating microRNA-889-3p-dependent FBXO4.

Applications

Unspecified application

Species

Unspecified reactive species

Haofeng Liu,Qiu Xue,Hongzhou Cai,Xiaohui Jiang,Guangxin Cao,Tie Chen,Yuan Chen,Ding Wang

International journal of oncology 60: PubMed34913066

2021

Chitinase 3-like 1 secreted from cancer-associated fibroblasts promotes tumor angiogenesis via interleukin-8 secretion in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kaori Watanabe,Kazuyoshi Shiga,Anri Maeda,Shinnosuke Harata,Takeshi Yanagita,Takuya Suzuki,Hajime Ushigome,Yuzo Maeda,Takahisa Hirokawa,Ryo Ogawa,Masayasu Hara,Hiroki Takahashi,Yoichi Matsuo,Akira Mitsui,Masahiro Kimura,Shuji Takiguchi

Histology and histopathology 37:227-241 PubMed34850965

2021

CircAKT3 promotes cell proliferation, survival and glutamine metabolism of gastric cancer by activating SLC1A5 expression via targeting miR-515-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Li,Lixiao Zhang,Qi Sun

International journal of clinical and experimental 13:1791-1801 PubMed32782707

2020

Circ_0016418 promotes melanoma development and glutamine catabolism by regulating the miR-605-5p/GLS axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijuan Lu,Xusheng Zhang,Xiaojuan Li,Xuefeng Wan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Glutaminase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody (AB260047)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

Western blot - Anti-Glutaminase antibody (AB260047)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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