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Anti-Glutaminase antibody [EP7212] (ab156876) is a rabbit monoclonal antibody that is used to detect Glutaminase in Western Blot. Suitable for Human samples.



- Specificity confirmed with Glutaminase knockout cell line validation


Images

Western blot - Anti-Glutaminase antibody [EP7212] (AB156876), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] (AB156876), expandable thumbnail
  • Western blot - Anti-Glutaminase antibody [EP7212] (AB156876), expandable thumbnail
  • Western blot - Anti-Glutaminase antibody [EP7212] (AB156876), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30239721, PubMed:30575854, PubMed:30970188). Isoform 2. Lacks catalytic activity.

Alternative names

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

Recommended products

Anti-Glutaminase antibody [EP7212] (ab156876) is a rabbit monoclonal antibody that is used to detect Glutaminase in Western Blot. Suitable for Human samples.



- Specificity confirmed with Glutaminase knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP7212
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Glutaminase antibody [EP7212] (ab156876) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), in Human samples.

What is the molecular weight of Glutaminase?


Anti-Glutaminase [EP7212] (ab156876) specifically detects a band for Glutaminase (UniProt: O94925) at a molecular weight of 73kDa.

Recommended positive controls


WB: Human kidney tissue lysates, HeLa and 293T cell lysates, wild type HAP1 cells.

Trusted by the scientific community


Anti-Glutaminase [EP7212] (ab156876) was first used in a scientific publication in 2013 and has been cited over 90 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-Glutaminase antibody [EP7212] (ab156876) has been confirmed by Western blot testing in GLS Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EP7212] also available for your convenience:
ab156876, HRP - HRP Anti-Glutaminase antibody [EP7212] ab200408, Carrier free - Anti-Glutaminase antibody [EP7212] - BSA and Azide free ab214802



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.

Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Associated diseases and disorders

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-Glutaminase antibody [EP7212] (ab156876), expandable thumbnail

    Western blot - Anti-Glutaminase antibody [EP7212] (ab156876)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg) Lane 2: GLS knockout HAP1 whole cell lysate (20 μg) Lane 3: HeLa whole cell lysate (20 μg) Lane 4: HEK293 whole cell lysate (20 μg) Lanes 1 - 4: Merged signal (red and green). Green - ab156876 observed at 62 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab156876 was shown to recognize GLS in wild-type HAP1 cells as signal was lost at the expected MW in HAP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS knockout samples were subjected to SDS-PAGE. ab156876 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    Blue arrow corresponds to isoform 1 KGA.

    Red arrow corresponds to isoform 3 GAC.

    All lanes: Western blot - Anti-Glutaminase antibody [EP7212] (ab156876)

    Predicted band size: 73 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] (ab156876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] (ab156876)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling Glutaminase with purified ab156876 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Glutaminase antibody [EP7212] (ab156876), expandable thumbnail

    Western blot - Anti-Glutaminase antibody [EP7212] (ab156876)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

    Observed band size: 65 kDa

    Exposure time: 1s

  • Western blot - Anti-Glutaminase antibody [EP7212] (ab156876), expandable thumbnail

    Western blot - Anti-Glutaminase antibody [EP7212] (ab156876)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

    Observed band size: 65 kDa

    Exposure time: 1s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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