JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB214802

Anti-Glutaminase antibody [EP7212] - BSA and Azide free

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal Glutaminase antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] - BSA and Azide free (AB214802)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] - BSA and Azide free (AB214802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling Glutaminase with purified ab156876 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156876).

Western blot - Anti-Glutaminase antibody [EP7212] - BSA and Azide free (AB214802)
  • WB

Lab

Western blot - Anti-Glutaminase antibody [EP7212] - BSA and Azide free (AB214802)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156876). Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutaminase antibody [EP7212] (<a href='/en-us/products/primary-antibodies/glutaminase-antibody-ep7212-ab156876'>ab156876</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65 kDa

true

Exposure time: 1s

Western blot - Anti-Glutaminase antibody [EP7212] - BSA and Azide free (AB214802)
  • WB

Lab

Western blot - Anti-Glutaminase antibody [EP7212] - BSA and Azide free (AB214802)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156876). Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutaminase antibody [EP7212] (<a href='/en-us/products/primary-antibodies/glutaminase-antibody-ep7212-ab156876'>ab156876</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65 kDa

true

Exposure time: 1s

  • Unconjugated

    Anti-Glutaminase antibody [EP7212]

  • HRP

    HRP Anti-Glutaminase antibody [EP7212]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7212

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab214802 is the carrier-free version of ab156876.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.
Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed : 30239721, PubMed : 30575854, PubMed : 30970188).. Isoform 2. Lacks catalytic activity.
See full target information GLS
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:7619-31 PubMed25844758

2015

Kidney-type glutaminase (GLS1) is a biomarker for pathologic diagnosis and prognosis of hepatocellular carcinoma.

Applications

IHC

Species

Human

Decai Yu,Xianbiao Shi,Gang Meng,Jun Chen,Chen Yan,Yong Jiang,Jiwu Wei,Yitao Ding

Molecular cancer therapeutics 13:890-901 PubMed24523301

2014

Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer.

Applications

Unspecified application

Species

Human

Matt I Gross,Susan D Demo,Jennifer B Dennison,Lijing Chen,Tania Chernov-Rogan,Bindu Goyal,Julie R Janes,Guy J Laidig,Evan R Lewis,Jim Li,Andrew L Mackinnon,Francesco Parlati,Mirna L M Rodriguez,Peter J Shwonek,Eric B Sjogren,Timothy F Stanton,Taotao Wang,Jinfu Yang,Frances Zhao,Mark K Bennett
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB260047

Anti-Glutaminase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody (AB260047)

  • 0
  • 0

Complementary Products

Assay Kits

AB284572

Glutamine Synthetase Activity Assay Kit (Colorimetric)

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB237704

Anti-SLC1A5/ASCT2 antibody [CAL33]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] (AB237704)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com