Rabbit Recombinant Monoclonal Glutaminase antibody. Carrier free. Suitable for Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide - Mouse, Human, Mouse, Rat samples.
Images
![Dot Blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--dot-blot-img429109.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--immunocytochemistry-immunofluorescence-img429148.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033)")
![Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--western-blot-img429081.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033)")
![Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--western-blot-img429082.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033)")
![Flow Cytometry (Intracellular) - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--flow-cytometry-intracellular-img429167.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (AB317033)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Expected | Tested | Expected | Expected | Not recommended | Not recommended |
| Synthetic peptide - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Mouse | Dilution info - | Notes - |
Target data
Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.
Alternative names
Gls1, Kiaa0838, GLS
Recommended products
Rabbit Recombinant Monoclonal Glutaminase antibody. Carrier free. Suitable for Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide - Mouse, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28716-72
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317033 is the carrirer-free version of Anti-Glutaminase antibody [EPR28716-72] ab317032.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.
- Biological function summary
Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.
- Pathways
Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.
- Associated diseases and disorders
Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Dot Blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--dot-blot-img429109.jpg "Dot Blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
Dot Blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)
Glutaminase Dot Blot staining using rabbit Anti-Glutaminase antibody
This data was developed using Anti-Glutaminase antibody [EPR28716-72] ab317032, the same antibody clone in a different buffer formulation.
Dot blot analysis of Glutaminase using Anti-Glutaminase antibody [EPR28716-72] ab317032 at 1:1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
This antibody does not cross-react with human/Mouse/Rat GLS2.
All lanes: Dot Blot - Anti-Glutaminase antibody [EPR28716-72] (Anti-Glutaminase antibody [EPR28716-72] ab317032) at 1/1000 dilution
Lane 1: Mouse GLS1 peptide a
Lane 2: Human GLS2 peptide b
Lane 3: Mouse/Rat GLS2 peptide c
Lane 4: Human GLS2 peptide d
Lane 5: Mouse/Rat GLS2 peptide e
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--immunocytochemistry-immunofluorescence-img429148.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)
This data was developed using Anti-Glutaminase antibody [EPR28716-72] ab317032, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling Glutaminase with Anti-Glutaminase antibody [EPR28716-72] ab317032 at 1/500 (1.016 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing mitochondrial staining in Neuro-2a cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--western-blot-img429081.jpg "Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)
This data was developed using Anti-Glutaminase antibody [EPR28716-72] ab317032, the same antibody clone in a different buffer formulation.
Negative control: liver (PMID:19428809).
This antibody could detect 2 isoforms produced by alternative splicing.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-5: 15 seconds, Lane 6: 3 seconds, Lane 7: 70 seconds
All lanes: Western blot - Anti-Glutaminase antibody [EPR28716-72] (Anti-Glutaminase antibody [EPR28716-72] ab317032) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate at 50 µg with 5% NFDM/TBST
Lane 2: Mouse liver tissue lysate at 50 µg with 5% NFDM/TBST
Lane 3: Rat kidney tissue lysate at 50 µg with 5% NFDM/TBST
Lane 4: Rat liver tissue lysate at 50 µg with 5% NFDM/TBST
Lane 5: Human liver tissue lysate at 50 µg with 5% NFDM/TBST
Lane 6: Human kidney tissue lysate at 20 µg with 5% NFDM/TBST
Lane 7: Human lung tissue lysate at 50 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 66 kDa, 73 kDa, 36 kDa
![Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--western-blot-img429082.jpg "Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
Western blot - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)
This data was developed using Anti-Glutaminase antibody [EPR28716-72] ab317032, the same antibody clone in a different buffer formulation.
This antibody could detect 2 isoforms produced by alternative splicing.
Exposure time: Lane 1-4: 10 seconds, Lane 5: 15 seconds
All lanes: Western blot - Anti-Glutaminase antibody [EPR28716-72] (Anti-Glutaminase antibody [EPR28716-72] ab317032) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg with 5% NFDM/TBST
Lane 2: B16-F10 (Mouse skin melanoma), whole cell lysate at 50 µg with 5% NFDM/TBST
Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST
Lane 4: G-401(human rhabdoid tumor kidney epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST
Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 50 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 66 kDa, 73 kDa
![Flow Cytometry (Intracellular) - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--flow-cytometry-intracellular-img429167.jpg "Flow Cytometry (Intracellular) - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
Flow Cytometry (Intracellular) - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)
This data was developed using Anti-Glutaminase antibody [EPR28716-72] ab317032, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Glutaminase with Anti-Glutaminase antibody [EPR28716-72] ab317032 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--immunocytochemistry-immunofluorescence-img429149.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)
This data was developed using Anti-Glutaminase antibody [EPR28716-72] ab317032, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Glutaminase with Anti-Glutaminase antibody [EPR28716-72] ab317032 at 1/500 (1.016 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing mitochondrial staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033), expandable thumbnail](https://content.abcam.com/products/images/glutaminase-antibody-epr28716-72-bsa-and-azide-free-ab317033--immunocytochemistry-immunofluorescence-img429149.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] - BSA and Azide free (ab317033)")
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