Anti-Glutaminase C antibody [EPR19525] (ab202027)

Rabbit Recombinant Monoclonal Glutaminase antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Images

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody [EPR19525] (AB202027), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Tested	Expected
Rat	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/600	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information GLS

Function

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30239721, PubMed:30575854, PubMed:30970188). Isoform 2. Lacks catalytic activity.

Alternative names

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

Recommended products

Rabbit Recombinant Monoclonal Glutaminase antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR19525
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glutaminase C also known as kidney-type glutaminase or GLS2 is an enzyme with a high molecular mass of approximately 65 kDa. This enzyme belongs to the mitochondrial compartment and carries out the conversion of glutamine to glutamate releasing ammonia in the process. It is expressed in various tissues including brain kidney and liver where it plays an important role in cellular metabolism. The enzyme demonstrates increased expression levels under specific conditions where cellular proliferation and energy requirements are high.

Biological function summary

Glutaminase C acts as an important player in nitrogen metabolism and cellular bioenergetics. It functions within the mitochondria facilitating the glutaminolysis pathway which provides intermediates for the tricarboxylic acid (TCA) cycle. Although glutaminase C mainly acts as a monomer in certain conditions it might participate in multiprotein complexes that aid its regulation and activity. This regulatory mechanism plays a vital role in energy homeostasis and cellular growth demands.

Pathways

Glutaminase C is involved in the glutaminolysis and TCA cycle pathways contributing significantly to energy production and biosynthesis in proliferating cells. It works in concert with related proteins like glutamate dehydrogenase which further converts the produced glutamate into α-ketoglutarate feeding into the TCA cycle. These combined actions support cellular adaptations during increased metabolic demand especially in cancerous cells where metabolic shifts are noticeable.

Associated diseases and disorders

Glutaminase C has a strong connection to cancer and neurological disorders. Cancer cells frequently exhibit heightened glutaminase C activity to sustain rapid growth associating with proteins such as c-Myc that drive metabolic reprogramming. Additionally in neurological ailments like epilepsy dysregulation of glutaminase C may alter neurotransmitter levels disturbing normal synaptic function. The enzyme's activity links to proteins involved in neuronal signaling suggesting its influence extends beyond mere metabolic processes.

Product promise

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8 product images

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glutaminase C with ab202027 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondrial staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). COX IV is detected with Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 (anti-COX IV(mouse mAb)) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glutaminase Cwith ab202027 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Blocking/Dilution buffer: 5% NFDM/TBST.
The observed expression profile is consistent with what has been described in UniProtKB (Glutaminase C is highly expressed in heart and pancreas, but is not detected in liver).
All lanes: Western blot - Anti-Glutaminase C antibody [EPR19525] (ab202027) at 1/1000 dilution
Lane 1: Human fetal heart lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Lane 3: Human fetal liver lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Glutaminase C with ab202027 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glutaminase C with ab202027 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Glutaminase was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab202027 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab202027 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10 μg (Input).
Lane 2: ab202027 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab202027 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Observed band size: 65 kDa
Western blot - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Glutaminase C antibody [EPR19525] (ab202027) at 1/1000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 5: Hepa1-6 (Mouse epithelial hepatoma cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 15s
Western blot - Anti-Glutaminase C antibody [EPR19525] (ab202027)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1 and 2: 3 minutes; Lane 3: 10 seconds; Lane 4, 5 and 6: 3 minutes; Lane 7: 30 seconds; Lane 8, 9 and 10: 15 seconds.
All lanes: Western blot - Anti-Glutaminase C antibody [EPR19525] (ab202027) at 1/5000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
Lane 5: Rat heart lysate at 10 µg
Lane 6: Rat spleen lysate at 10 µg
Lane 7: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 8: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 9: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 10: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa