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AB64613

Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free

4

(8 Reviews)

|

(80 Publications)

Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) is a mouse monoclonal antibody for Western Blot, Flow Cytometry, IHC-P, ICC/IF, ELISA. Suitable for Drosophila melanogaster, Human, Mouse, Rat, Zebrafish.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 50 publications
- Trusted since 2008

View Alternative Names

GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

IHC image of ab64613 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64613, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

Overlay histogram showing Jurkat cells stained with ab64613 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64613, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

ICC image of ab64613 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64613, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • WB

Supplier Data

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613)

All lanes:

HepG2 cell lysate

Predicted band size: 42 kDa

false

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • WB

Unknown

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 42 kDa

Observed band size: 37 kDa

false

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • WB

Unknown

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) at 0.5 µg/mL

Lane 1:

Marker

Lane 2:

Zebrafish brain homogenate at 20 µg

Lane 3:

Zebrafish liver homogenate at 20 µg

Lane 4:

Zebrafish skeletal muscle homogenate at 20 µg

Lane 5:

JURKAT (Human T cell lymphoblast-like cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

true

Exposure time: 4min

Sandwich ELISA - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)

Detection limit for recombinant GST tagged Glutamine Synthetase is approximately 0.03ng/ml as a capture antibody.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B6

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Drosophila melanogaster, Zebrafish

Applications

WB, sELISA, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GLUL aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15104

Reactivity data

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"predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", 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Product details

What is this antibody validated in?
Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Drosophila melanogaster, Human, Mouse, Rat, Zebrafish samples.

What is the molecular weight of Glutamine Synthetase?
Anti-Glutamine Synthetase [3B6] - BSA and Azide free (ab64613) specifically detects a band for Glutamine Synthetase (UniProt: P15104) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-Glutamine Synthetase [3B6] - BSA and Azide free (ab64613) was first used in a scientific publication in 2008 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Glutamine Synthetase [3B6] - BSA and Azide free (ab64613) has over 5 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.
Biological function summary

This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.

Pathways

Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.

Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed : 16267323, PubMed : 30158707, PubMed : 36289327). Its role depends on tissue localization : in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Plays a key role in ammonium detoxification during erythropoiesis : the glutamine synthetase activity is required to remove ammonium generated by porphobilinogen deaminase (HMBS) during heme biosynthesis to prevent ammonium accumulation and oxidative stress (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed : 18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development : acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed : 30158707). May act as a palmitoyltransferase for RHOJ : able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed : 30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed : 26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed : 36289327).
See full target information GLUL

Publications (80)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1623211 PubMed41035640

2025

HDAC inhibitor MS275 reprograms metabolism to induce differentiation and suppress proliferation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjie Li,Cheng Hu,Yuyu Ye,Song Wei,Wenbo Zhu,Jiankai Liang,Jing Cai,Yuan Lin,Liang Peng,Guangmei Yan,Ying Liu

Nature communications 16:8262 PubMed40998803

2025

Targeting prostaglandin E receptor 2 in Schwann cells inhibits inflammatory pain but not inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Nassini,Lorenzo Landini,Matilde Marini,Martina Chieca,Daniel Souza Monteiro de Araújo,Marco Montini,Pasquale Pensieri,Vittorio Donato Abruzzese,Gaetano De Siena,Jin Zhang,Elisa Bellantoni,Vincenzo De Giorgi,Antonia Romitelli,Giulia Brancolini,Raquel Tonello,Chloe J Peach,Alessandra Mastricci,Irene Scuffi,Martina Tesi,Dane D Jensen,Brian L Schmidt,Nigel W Bunnett,Francesco De Logu,Pierangelo Geppetti

Journal of biomedical research :1-14 PubMed40770862

2025

HDGF derived from Müller cells enhances the activation of microglia in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Aowang Qiu,Wenjie Yin,Ningyu Wang,Xin Wang,Qinghuai Liu,Weiwei Zhang

Pain 166:2054-2066 PubMed39907482

2025

Potentiation of visualized exosomal miR-1306-3p from primary sensory neurons contributes to chronic visceral pain via spinal P2X3 receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Rui-Xia Weng,Yong-Chang Li,Shu-Man Jia,Chun-Tao Ma,Hong-Hong Zhang,Yong Tang,Rui Li,Guang-Yin Xu

Investigative ophthalmology & visual science 66:62 PubMed39873651

2025

SIRT4 Protects Retina Against Excitotoxic Injury by Promoting OPA1-Mediated Müller Glial Cell Mitochondrial Fusion and GLAST Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Ying,Hongdou Luo,Zhi Xie,Yi Huang,Haijian Hu,Ming Jin,Ke Xu,Yulian Pang,Yuning Song,Xu Zhang

Neural regeneration research 20:2682-2696 PubMed39503430

2024

Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhang,Yuqi Liu,Fangxia Xu,Chengcheng Zhou,Kaimei Lu,Bin Fang,Lijuan Wang,Lina Huang,Zifeng Xu

EMBO reports 25:5429-5455 PubMed39448865

2024

Histone modifications and Sp1 promote GPR160 expression in bone cancer pain within rodent models.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfei Xu,Yahui Wang,Chaobo Ni,Miao Xu,Chengyu Yin,Qiuli He,Bing Ma,Jie Fu,Baoxia Zhao,Liping Chen,Tong Zhi,Shirong Wei,Liang Cheng,Hui Xu,Jiajun Xiao,Lei Yang,Qingqing Xu,Jiao Kuang,Boyi Liu,Qinghe Zhou,Xuewu Lin,Ming Yao,Huadong Ni

International journal of molecular sciences 25: PubMed38891784

2024

Constitutive Neurogenesis and Neuronal Plasticity in the Adult Cerebellum and Brainstem of Rainbow Trout, .

Applications

Unspecified application

Species

Unspecified reactive species

Evgeniya Vladislavovna Pushchina,Anatoly Alekseevich Varaksin

Cell communication and signaling : CCS 22:307 PubMed38831315

2024

Interleukin-22 receptor 1-mediated stimulation of T-type Ca channels enhances sensory neuronal excitability through the tyrosine-protein kinase Lyn-dependent PKA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Cai,Siyu Chen,Yufang Sun,Tingting Zheng,Yulu Liu,Jin Tao,Yuan Zhang

Investigative ophthalmology & visual science 65:36 PubMed38776115

2024

CD38 Deficiency Protects Mouse Retinal Ganglion Cells Through Activating the NAD+/Sirt1 Pathway in Ischemia-Reperfusion and Optic Nerve Crush Models.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Pang,Haijian Hu,Ke Xu,Ting Cao,Zhiruo Wang,Jiahe Nie,Haina Zheng,Hongdou Luo,Feifei Wang,Chan Xiong,Ke-Yu Deng,Hong-Bo Xin,Xu Zhang
View all publications

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