Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free
- BOND RX™ Validated
- What is this?
4
(8 Reviews)
|
(80 Publications)
Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) is a mouse monoclonal antibody for Western Blot, Flow Cytometry, IHC-P, ICC/IF, ELISA. Suitable for Drosophila melanogaster, Human, Mouse, Rat, Zebrafish.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 50 publications
- Trusted since 2008
View Alternative Names
GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
IHC image of ab64613 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64613, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image was generated using the ascites version of the product.
- Flow Cyt
Unknown
Flow Cytometry - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
Overlay histogram showing Jurkat cells stained with ab64613 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64613, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
ICC image of ab64613 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64613, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.
- WB
Supplier Data
Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
This image was generated using the ascites version of the product.
All lanes:
Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613)
All lanes:
HepG2 cell lysate
Predicted band size: 42 kDa
false
- WB
Unknown
Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
This image was generated using the ascites version of the product.
All lanes:
Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) at 1 µg/mL
All lanes:
Jurkat cell lysate at 50 µg
Secondary
All lanes:
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 42 kDa
Observed band size: 37 kDa
false
- WB
Unknown
Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
This image was generated using the ascites version of the product.
All lanes:
Western blot - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) at 0.5 µg/mL
Lane 1:
Marker
Lane 2:
Zebrafish brain homogenate at 20 µg
Lane 3:
Zebrafish liver homogenate at 20 µg
Lane 4:
Zebrafish skeletal muscle homogenate at 20 µg
Lane 5:
JURKAT (Human T cell lymphoblast-like cell line) whole cell lysate at 20 µg
Secondary
All lanes:
Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
true
Exposure time: 4min
- sELISA
Supplier Data
Sandwich ELISA - Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (AB64613)
Detection limit for recombinant GST tagged Glutamine Synthetase is approximately 0.03ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
Reactivity data
Product details
Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Drosophila melanogaster, Human, Mouse, Rat, Zebrafish samples.
What is the molecular weight of Glutamine Synthetase?
Anti-Glutamine Synthetase [3B6] - BSA and Azide free (ab64613) specifically detects a band for Glutamine Synthetase (UniProt: P15104) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-Glutamine Synthetase [3B6] - BSA and Azide free (ab64613) was first used in a scientific publication in 2008 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Glutamine Synthetase [3B6] - BSA and Azide free (ab64613) has over 5 independent reviews from customers.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.
Pathways
Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (80)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in immunology 16:1623211 PubMed41035640
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 16:8262 PubMed40998803
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of biomedical research :1-14 PubMed40770862
2025
Applications
Unspecified application
Species
Unspecified reactive species
Pain 166:2054-2066 PubMed39907482
2025
Applications
Unspecified application
Species
Unspecified reactive species
Investigative ophthalmology & visual science 66:62 PubMed39873651
2025
Applications
Unspecified application
Species
Unspecified reactive species
Neural regeneration research 20:2682-2696 PubMed39503430
2024
Applications
Unspecified application
Species
Unspecified reactive species
EMBO reports 25:5429-5455 PubMed39448865
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 25: PubMed38891784
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cell communication and signaling : CCS 22:307 PubMed38831315
2024
Applications
Unspecified application
Species
Unspecified reactive species
Investigative ophthalmology & visual science 65:36 PubMed38776115
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com