AB181625

Anti-Glutamine Synthetase antibody

Name: Anti-Glutamine Synthetase antibody (ab181625) | Abcam
Brand: Abcam Limited
SKU: AB181625
Price: 535 USD
Availability: InStock

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(1 Publication)

Goat Polyclonal Glutamine Synthetase antibody. Suitable for WB and reacts with Brevibacterium samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human GLUL.

View Alternative Names

GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL

2 Images

Supplier Data

Western blot - Anti-Glutamine Synthetase antibody (AB181625)

All lanes:

Western blot - Anti-Glutamine Synthetase antibody (ab181625) at 1/1000 dilution

All lanes:

Glutamine Synthetase at 0.05 µg

Secondary

All lanes:

Peroxidase anti-Goat at 1/40000 dilution

Predicted band size: 42 kDa

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Supplier Data

Western blot - Anti-Glutamine Synthetase antibody (AB181625)

Other band(s) : Glutamine Synthetase splice variants and isoforms.

All lanes:

Western blot - Anti-Glutamine Synthetase antibody (ab181625) at 1/3000 dilution

Lane 1:

Glutamine Synthetase Reduced at 1 µg

Lane 2:

Glutamine Synthetase Non-reduced at 1 µg

Secondary

All lanes:

Dylight 649 conjugated Donkey anti goat at 1/10000 dilution

Predicted band size: 42 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Brevibacterium

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human GLUL.

P15104

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Brevibacterium": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification technique

Ion exchange chromatography

Purification notes

Anti-Glutamine Synthetase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.

Biological function summary

This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.

Pathways

Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.

Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed : 16267323, PubMed : 30158707, PubMed : 36289327). Its role depends on tissue localization : in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed : 18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development : acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed : 30158707). May act as a palmitoyltransferase for RHOJ : able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed : 30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed : 26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed : 36289327).

See full target information GLUL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 26:2514-2532 PubMed33303946

2020

ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Huang,Jing Huang,Qi-Xin Zhou,Chun-Xian Yang,Cui-Ping Yang,Wan-Ying Mei,Lei Zhang,Qiong Zhang,Ling Hu,Yun-Qing Hu,Ning-Ning Song,Sheng-Xi Wu,Lin Xu,Yu-Qiang Ding

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Glutamine Synthetase antibody

Western blot - Anti-Glutamine Synthetase antibody (AB181625)

Western blot - Anti-Glutamine Synthetase antibody (AB181625)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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