Skip to main content

Rabbit Polyclonal Glutamine Synthetase antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GLNA_HUMAN aa 250-350.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-Glutamine Synthetase antibody (AB264395), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human GLNA_HUMAN aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link P15104

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IP
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Cow
Predicted
Dog
Predicted

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Dog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:16267323, PubMed:30158707, PubMed:36289327). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed:36289327).

Alternative names

Recommended products

Rabbit Polyclonal Glutamine Synthetase antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GLNA_HUMAN aa 250-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human GLNA_HUMAN aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link P15104
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.

Biological function summary

This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.

Pathways

Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.

Associated diseases and disorders

Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunoprecipitation - Anti-Glutamine Synthetase antibody (ab264395), expandable thumbnail

    Immunoprecipitation - Anti-Glutamine Synthetase antibody (ab264395)

    Glutamine Synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264395 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264395 at 0.4 μg/ml.

    Lane 1: ab264395 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

    Lysates prepared using NETN lysis buffer.

    All lanes: Immunoprecipitation - Anti-Glutamine Synthetase antibody (ab264395)

    Predicted band size: 42 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com