Anti-Glutamine Synthetase antibody

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (AB73593)

Immunohistochemistical detection of Glutamine Synthetase on Formaldehyde-fixed paraffin-embedded rat brain sections using Glutamine Synthetase antibody (ab73593). Antigen retrieval step : Heat mediated - Buffer/Enzyme Used : Citric acid pH6 Permeabilization. Blocking : 1% BSA for 10 mins at room temperature. Primary Antibody Dilution 1/1000. Incubation time : 2 hours in TBS/BSA/azide. Secondary Antibody : anti Rabbit IgG Conjugated to biotin (1/200). In the submitted image of rat cerebellum, two very good examples are observed of the bulbous endfeet of the primary Bergmann glia primary processes connecting with the inner Pial membrane.

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (AB73593)

IHC image of Glutamine Synthetase staining in a section of formalin-fixed paraffin-embedded normal rat liver performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab73593, 1 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (AB73593)

IHC image of Glutamine Synthetase staining in a section of formalin-fixed paraffin-embedded normal rat cerebellum performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab73593, 1 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody (AB73593)

ab73593 staining Glutamine Synthetase in MEF1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab73593 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

Project

Western blot - Anti-Glutamine Synthetase antibody (AB73593)

All lanes:

Western blot - Anti-Glutamine Synthetase antibody (ab73593) at 1 µg/mL

Lane 1:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

Liver (Rat) Tissue Lysate at 10 µg

Lane 4:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 5:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 6:

Lung (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Anti-Glutamine Synthetase antibody (AB73593)

All lanes:

Western blot - Anti-Glutamine Synthetase antibody (ab73593) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Glutamine Synthetase protein (<a href='/en-us/products/proteins-peptides/recombinant-human-glutamine-synthetase-protein-ab98145'>ab98145</a>) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 42 kDa

true

Exposure time: 2min