AB317188

Anti-Glutamine synthetase antibody [HL2283]

Name: Anti-Glutamine synthetase antibody [HL2283] (ab317188) | Abcam
Brand: Abcam Limited
SKU: AB317188

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Rabbit Monoclonal Glutamine Synthetase antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse, Rat, Dog, Cat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GLUL.

View Alternative Names

GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

ab317188 detects Glutamine synthetase protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. Glutamine synthetase stained by ab317188 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

ab317188 detects Glutamine synthetase protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. Glutamine synthetase stained by ab317188 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317188 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Glutamine synthetase antibody [HL2283] (ab317188) at 1/2000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

SK-MEL-28 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317188 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Glutamine synthetase antibody [HL2283] (ab317188) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

GLUL shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317188 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Glutamine synthetase antibody [HL2283] (ab317188) at 1/5000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

ab317188 detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded cat cerebellum. Glutamine synthetase stained by ab317188 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

Whole cell extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317188 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Glutamine synthetase antibody [HL2283] (ab317188) at 1/1000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Glutamine synthetase antibody [HL2283] (AB317188)

All lanes:

Western blot - Anti-Glutamine synthetase antibody [HL2283] (ab317188) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged GLUL transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2283

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat, Dog, Cat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GLUL.

P15104

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate-ammonia ligase also known as glutamine synthetase is an enzyme important for the synthesis of glutamine from glutamate and ammonia. This enzyme operates by catalyzing the ATP-dependent conversion playing a significant role in nitrogen metabolism. The human form of this enzyme has a mass of approximately 42 kDa. Glutamate-ammonia ligase is widely expressed found in tissues including the liver brain and kidneys pointing to its importance in diverse physiological processes.

Biological function summary

Glutamate-ammonia ligase performs an important function in nitrogen metabolism by catalyzing the reaction that produces glutamine a vital amino acid and a source of cellular carbon and nitrogen. This enzyme also participates in the detoxification of ammonia a process critical in the brain. It is known to operate as part of a larger enzyme complex working intricately with other cellular components to maintain nitrogen balance and detoxify harmful metabolic byproducts.

Pathways

The glutamate-ammonia ligase enzyme plays an integral role in the urea cycle and the glutamine-glutamate cycle. The urea cycle is pivotal in converting potentially harmful ammonia to urea for safe excretion showing close interaction with carbamoyl phosphate synthetase. In the glutamine-glutamate cycle the enzyme assists in neurotransmitter cycling and nitrogen metabolism interacting with proteins like glutaminase which converts glutamine back to glutamate.

Glutamate-ammonia ligase involvement links to conditions such as hepatic encephalopathy and neurodegenerative diseases like Alzheimer's disease. In hepatic encephalopathy enzyme dysfunction leads to impaired ammonia detoxification contributing to the neurological symptoms associated with liver disease. In neurodegenerative contexts altered glutamine and glutamate metabolism involve proteins such as amyloid precursor protein highlighting the enzyme's influence on brain health and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed : 16267323, PubMed : 30158707, PubMed : 36289327). Its role depends on tissue localization : in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed : 18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development : acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed : 30158707). May act as a palmitoyltransferase for RHOJ : able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed : 30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed : 26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed : 36289327).

See full target information GLUL

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