Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody. (Red) compared to a nonspecific negative control
antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

Immunohistochemical analysis of human breast tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell using using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody. (Red) compared to a nonspecific negative control antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

Immunohistochemical analysis of human endometrum tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

• WB

Supplier Data

Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

All lanes:

Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 42 kDa

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

Immunofluorescence analysis of COS-7 (african green monkey kidney fibroblast-like cell line) whole cell labeling Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody..

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

• WB

Supplier Data

Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (AB273647)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

All lanes:

Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY GLUL-transfected HEK-293T whole cell lysate at 5 µg

Predicted band size: 42 kDa

false