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AB221121

Anti-Glutaredoxin 5 antibody

1

(1 Review)

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(1 Publication)

Rabbit Polyclonal Glutaredoxin 5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GLRX5 aa 100 to C-terminus.

View Alternative Names

C14orf87, GLRX5, Monothiol glutaredoxin-5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 5 antibody (AB221121)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 5 antibody (AB221121)

Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized RH30 cells labeling Glutaredoxin 5 with ab221121 at 4 μg/ml (green).

Western blot - Anti-Glutaredoxin 5 antibody (AB221121)
  • WB

Supplier Data

Western blot - Anti-Glutaredoxin 5 antibody (AB221121)

All lanes:

Western blot - Anti-Glutaredoxin 5 antibody (ab221121) at 1/100 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GLRX5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86SX6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaredoxin 5 also known as GRX5 is a mitochondrial protein with a molecular mass of approximately 17 kDa. It efficiently catalyzes the reduction of disulfide bonds in substrate proteins through a thiol-disulfide exchange mechanism. GRX5 is highly expressed in tissues with active metabolism such as heart liver and skeletal muscles. This protein belongs to the glutaredoxin family which are small redox enzymes that use glutathione as a cofactor.
Biological function summary

The function of GRX5 revolves around iron-sulfur (Fe-S) cluster assembly. It is part of a multiprotein complex involved in the maturation of Fe-S proteins within the mitochondria. These iron-sulfur clusters are essential for various cellular processes including enzymatic functions and electron transport. The loss of GRX5 activity disrupts Fe-S cluster assembly leading to compromised cellular function and increased oxidative stress.

Pathways

GRX5 plays a significant role in the biosynthesis of Fe-S clusters. It is closely connected to the ISC assembly machinery an important pathway for the formation of these clusters. In this context GRX5 interacts with proteins such as ISCU and NFU1 contributing to the proper maturation and stability of Fe-S proteins which are pivotal for mitochondrial electron transport chain function and DNA repair mechanisms.

GRX5 dysregulation is linked to autosomal recessive sideroblastic anemia and mitochondrial dysfunction. This disorder results from defective Fe-S cluster biosynthesis leading to impaired haem synthesis and mitochondrial iron accumulation. GRX5's interaction with frataxin another protein involved in Fe-S cluster assembly further elucidates its role in the pathophysiology of diseases like Friedreich's ataxia where deficient iron-sulfur cluster synthesis leads to neurodegeneration and systemic abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed : 20364084, PubMed : 23615440). Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed : 20364084, PubMed : 23615440, PubMed : 24334290). Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed : 20364084).
See full target information GLRX5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of assisted reproduction and genetics 42:923-936 PubMed39810070

2025

miR-361-5p regulates SLC25A24 to maintain mitochondrial function and alleviate granulosa cell dysfunction in diminished ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyuan Xu,Yan Jia
View all publications
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Product promise

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