Rabbit Polyclonal Glutaredoxin 5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GLRX5 aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GLRX5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q86SX6
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed:20364084, PubMed:23615440). Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed:20364084, PubMed:23615440, PubMed:24334290). Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed:20364084).
Alternative names
C14orf87, GLRX5, Monothiol glutaredoxin-5
Recommended products
Rabbit Polyclonal Glutaredoxin 5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GLRX5 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GLRX5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q86SX6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutaredoxin 5 also known as GRX5 is a mitochondrial protein with a molecular mass of approximately 17 kDa. It efficiently catalyzes the reduction of disulfide bonds in substrate proteins through a thiol-disulfide exchange mechanism. GRX5 is highly expressed in tissues with active metabolism such as heart liver and skeletal muscles. This protein belongs to the glutaredoxin family which are small redox enzymes that use glutathione as a cofactor.
- Biological function summary
The function of GRX5 revolves around iron-sulfur (Fe-S) cluster assembly. It is part of a multiprotein complex involved in the maturation of Fe-S proteins within the mitochondria. These iron-sulfur clusters are essential for various cellular processes including enzymatic functions and electron transport. The loss of GRX5 activity disrupts Fe-S cluster assembly leading to compromised cellular function and increased oxidative stress.
- Pathways
GRX5 plays a significant role in the biosynthesis of Fe-S clusters. It is closely connected to the ISC assembly machinery an important pathway for the formation of these clusters. In this context GRX5 interacts with proteins such as ISCU and NFU1 contributing to the proper maturation and stability of Fe-S proteins which are pivotal for mitochondrial electron transport chain function and DNA repair mechanisms.
- Associated diseases and disorders
GRX5 dysregulation is linked to autosomal recessive sideroblastic anemia and mitochondrial dysfunction. This disorder results from defective Fe-S cluster biosynthesis leading to impaired haem synthesis and mitochondrial iron accumulation. GRX5's interaction with frataxin another protein involved in Fe-S cluster assembly further elucidates its role in the pathophysiology of diseases like Friedreich's ataxia where deficient iron-sulfur cluster synthesis leads to neurodegeneration and systemic abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 5 antibody (ab221121)
Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized RH30 cells labeling Glutaredoxin 5 with ab221121 at 4 μg/ml (green).
Western blot - Anti-Glutaredoxin 5 antibody (ab221121)
All lanes: Western blot - Anti-Glutaredoxin 5 antibody (ab221121) at 1/100 dilution
Lane 1: RT4 cell lysate
Lane 2: U251 MG cell lysate
Predicted band size: 17 kDa
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