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Mouse Monoclonal Glutathione antibody. Suitable for Flow Cyt, ICC/IF and reacts with Chemical samples. Cited in 46 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Glutathione.


Images

Flow Cytometry - Anti-Glutathione antibody [D8] (AB19534), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.2 - 7.6
Preservative: 0.01% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Chemical / Small Molecule corresponding to Glutathione.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Chemical
Tested
Tested

Tested
Tested

Species

Chemical

Dilution info

-

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Chemical

Dilution info

10 µg/mL

Notes

-

Alternative names

Recommended products

Mouse Monoclonal Glutathione antibody. Suitable for Flow Cyt, ICC/IF and reacts with Chemical samples. Cited in 46 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Glutathione.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Chemical / Small Molecule corresponding to Glutathione.
Clone number

D8

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glutathione often referred to as GSH is a small tripeptide with a molecular mass of approximately 307.33 g/mol. It is composed of amino acids glutamine cysteine and glycine. Glutathione is found in almost all cells with high concentrations especially in the liver. As a powerful antioxidant it functions by reducing reactive oxygen species (ROS) and maintaining the redox status of cells. Glutathione exists in two forms: reduced (GSH) and oxidized (GSSG). The balance between these forms known as the GSH/GSSG ratio is critical for cellular health and is commonly assessed using a glutathione assay kit.

Biological function summary

Glutathione plays an important role in detoxification processes protecting cells from damage caused by free radicals peroxides and heavy metals. It acts as a substrate for various enzymes like glutathione peroxidase and glutathione S-transferase involved in neutralizing oxidative damage. Glutathione doesn't typically form a complex but it does participate in disulfide bond exchange reactions which are critical in maintaining cellular protein functions and structures.

Pathways

Glutathione is integral to cellular antioxidant defense and phase II detoxification pathways. It closely interacts with proteins like glutathione peroxidase in the reduction of hydrogen peroxide to water. Also glutathione's role in the pentose phosphate pathway supports the regeneration of NADPH which is essential for maintaining the reduced form of glutathione. This interplay is important for protecting cells against oxidative stress and ensuring cellular energy is balanced.

Associated diseases and disorders

Glutathione has significant implications in conditions such as Parkinson's disease and liver cirrhosis. In Parkinson's disease researchers observe lower levels of glutathione in affected brain regions suggesting an important role in neurodegeneration. Glutathione levels also impact liver health with deficiencies leading to ineffective detoxification and the potential for liver damage or cirrhosis. In these contexts glutathione interacts with proteins involved in redox balance and neurotransmitter regulation emphasizing its broad significance in maintaining human health.

Product promise

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4 product images

  • Flow Cytometry - Anti-Glutathione antibody [D8] (ab19534), expandable thumbnail
    Image courtesy of Dr Mahesh Shivananjappa by customer review.

    Flow Cytometry - Anti-Glutathione antibody [D8] (ab19534)

    ab19534 at a 1/250 dilution detecting Glutathione in human monocytes by Flow Cytometry. An Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) secondary was used at a 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534), expandable thumbnail
    Image courtesy of Dr Mahesh Shivananjappa by customer review.

    Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)

    ab19534 staining Glutathione in cultured murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab19534 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)

    ab19534 staining glutathione in A549 cells treated with apocynin (Apocynin, NADPH-oxidase inhibitor ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of Apocynin, NADPH-oxidase inhibitor ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 μg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534), expandable thumbnail
    Image from Lim SY et al, J Biol Chem. 2010 May 7;285(19):14377-88. Epub 2010 Mar 11, Fig 4.

    Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)

    ab19534 staining Glutathione in human neutrophils by Immunocytochemistry/ Immunofluorescence.



    Samples were fixed with 4% (w/v) paraformaldehyde in PBS, permeabilized with PBS containing 0.5% (w/v) saponin and 0.1% (w/v) bovine serum albumin, and then blocked with 1% (w/v) bovine serum albumin in PBS for 1 hour. ab19534 was used at 5μg/ml for 2 hours at room temperature. After washing with PBS, cells were incubated with Alexa 488-conjugated anti-mouse IgG at a 1/200 dilution.

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Product protocols

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