Rabbit Polyclonal Glutathione Peroxidase 1 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 151 publications.
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- International journal of molecular sciences 24:2023Prenatal High-Fat Diet Combined with Microplastic Exposure Induces Liver Injury via Oxidative Stress in Male Pups.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMao-Meng Tiao,Jiunn-Ming Sheen,I-Chun Lin,Madalitso Khwepeya,Hong-Ren YuPubMed 37686267
- International journal of molecular sciences 24:2023Landscape of Well-Coordinated Fracture Healing in a Mouse Model Using Molecular and Cellular Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeeksha Malhan,Katharina Schmidt-Bleek,Georg N Duda,Thaqif El KhassawnaPubMed 36834981
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 56:e124082023Changes of antioxidant enzymes in the kidney after cardiac arrest in the rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ H Lee,M S Islam,Y J Yoo,S E Kim,R H Kim,Y J Jang,S H Lee,H P Hwang,H Y Shin,J H Hwang,K Kim,B Y Park,D Ahn,Y Lee,T Kim,I S Kim,J C Yoon,H J TaePubMed 36790289
- Bioactive materials 23:261-2732022Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria P Kwesiga,Amani A Gillette,Fatemeh Razaviamri,Margaret E Plank,Alexia L Canull,Zachary Alesch,Weilue He,Bruce P Lee,Roger J GuilloryPubMed 36439083
- International journal of molecular sciences 23:2022Immunoproteasome Inhibition Ameliorates Aged Dystrophic Mouse Muscle Environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuana Tripodi,Davide Molinaro,Francesco Fortunato,Carolina Mella,Barbara Cassani,Yvan Torrente,Andrea FariniPubMed 36498987
- Cancers 14:2022High Hemin Concentration Induces Escape from Senescence of Normoxic and Hypoxic Colon Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgata Borkowska,Aleksandra Olszewska,Weronika Skarzynska,Marta Marciniak,Maciej Skrzeszewski,Claudine Kieda,Halina WasPubMed 36230727
- International journal of molecular sciences 23:2022Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanica Tasić,Miloš Opačić,Sanja Kovačević,Aleksandra Nikolić Kokić,Milena Dimitrijević,Dušan Nikolić,Danijela Vojnović Milutinović,Duško Blagojević,Ana Djordjevic,Jelena BrkljačićPubMed 36012287
- Journal of animal science 100:2022The impact of Zearalenone on heat-stressed skeletal muscle in pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTori E Rudolph,Crystal M Roach,Lance H Baumgard,Jason W Ross,Aileen F Keating,Josh T SelsbyPubMed 35908787
- Journal of inflammation research 15:4217-42382022Extracellular Histones Activate Endothelial NLRP3 Inflammasome and are Associated with a Severe Sepsis Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJesús Beltrán-García,Rebeca Osca-Verdegal,Daniel Pérez-Cremades,Susana Novella,Carlos Hermenegildo,Federico V Pallardó,José Luis García-GiménezPubMed 35915852
- Theranostics 12:5451-54692022Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Wang,Mingzhi Han,Shuai Wang,Yanfei Sun,Wenbo Zhao,Zhiyi Xue,Xiangjun Liang,Bin Huang,Gang Li,Anjing Chen,Xingang Li,Jian WangPubMed 35910786
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Cow | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism (PubMed:11115402).
Alternative names
Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1
Recommended products
Rabbit Polyclonal Glutathione Peroxidase 1 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 151 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab22604 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutathione Peroxidase 1 (GPx1) also known as GSH peroxidase is a selenium-containing enzyme with a mass of approximately 22 kDa. It plays an important role in reducing hydrogen peroxide to water using glutathione as a substrate therefore protecting cells from oxidative damage. GPx1 is widely expressed in many tissues but is found in high concentrations in the liver and erythrocytes. Researchers use antibodies specific to GPx1 often obtained from specialized suppliers to study and quantify this enzyme in various biological samples.
- Biological function summary
The enzyme guards cellular components by neutralizing free radicals. GPx1 is not part of a large complex but functions as a homotetramer. Its activity is essential for preserving the redox balance within the cell preventing cellular damage from reactive oxygen species (ROS). Enzyme-linked immunosorbent assays (ELISA) often measure GPx1 activity to assess oxidative stress levels in research and clinical settings.
- Pathways
GPx1 is important in the glutathione metabolism and cellular antioxidant defense pathways. Within these pathways GPx1 works closely with proteins like glutathione reductase to maintain reduced glutathione levels. This interplay with other antioxidant proteins ensures cellular protection against oxidative damage. Studies focus on GPx1's role in these pathways as it underpins many critical cellular processes maintaining cell health.
- Associated diseases and disorders
Decreased GPx1 activity links to neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases such as Alzheimer's the oxidative stress overwhelms the antioxidant defenses including GPx1. Similarly in cardiovascular disorders a diminished GPx1 activity correlates with increased oxidative stress contributing to disease progression. Researchers also investigate connections between GPx1 and other oxidative stress-related proteins like superoxide dismutase to fully understand GPx1's role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 1 antibody (ab22604)
ab22604 staining Glutathione Peroxidase 1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab22604 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 μg)
Lane 3: THP1 cell lysate (20 μg)
Lane 4: HL60 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab22604 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab22604 was shown to recognize Glutathione Peroxidase 1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab22604 at a concentration of 1μg/ml and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) diluted at 1/2000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
Predicted band size: 22 kDa, 47 kDa
Observed band size: 47 kDa
This image is courtesy of a customer review submitted by Carl HobbsImmunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 1 antibody (ab22604)
ab22604 staining mouse Cor-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with TBS/BSA/azide/0.1%Tween 20 and blocked with 1% BSA for 10 minutes at RT. Samples were incubated with primary antibody (1/300) for 2 hours. An diluted (1/1000) Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab22604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 190 kDa, 22 kDa, 55 kDa, 65 kDa
Exposure time: 4min
Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
ab22604 detects a band of approximately 22 kDa in human liver lysate. The band detected in the mouse liver lysate runs slightly higher (~ 24kDa). We believe that these bands correspond to Glutathione Peroxidase 1 as they are both blocked by the addition of the immunizing peptide (ab25301).
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/mL
Lanes 1 and 3: Human liver normal tissue lysate at 20 µg
Lanes 2 and 4: Liver (Mouse) Tissue Lysate (ab7935) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa, 24 kDa
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