Rabbit Recombinant Monoclonal Glutathione Peroxidase 1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.
Images
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (AB108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img166403.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (AB108429)")
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (AB108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img57573.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (AB108429)")
![Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (AB108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--flow-cytometry-intracellular-img37361.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (AB108429)")
Publications
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- Redox biology 58:1025342022SRT1720 as an SIRT1 activator for alleviating paraquat-induced models of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Chang Chao,Chuen-Lin Huang,Jing-Jy Cheng,Chun-Tang Chiou,I-Jung Lee,Ying-Chen Yang,Ting-Huang Hsu,Chia-En Yei,Pei-Ying Lin,Jih-Jung Chen,Nai-Kuei HuangPubMed 36379180
- Cancer research 82:3486-34982022Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConnor S R Jankowski,Joshua D RabinowitzPubMed 35916672
- Redox biology 55:1023982022Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngel Gallego-Selles,Victor Galvan-Alvarez,Miriam Martinez-Canton,Eduardo Garcia-Gonzalez,David Morales-Alamo,Alfredo Santana,Juan Jose Gonzalez-Henriquez,Cecilia Dorado,Jose A L Calbet,Marcos Martin-RinconPubMed 35841628
- Frontiers in molecular biosciences 8:8066502022Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Liang,Shuohui Dong,Wenwen Liu,Man Wang,Shanshan Tian,Yu Ai,Haibo WangPubMed 35096971
- International journal of molecular sciences 22:2021Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan Sonet,Anne-Laure Bulteau,Zahia Touat-Hamici,Maurine Mosca,Katarzyna Bierla,Sandra Mounicou,Ryszard Lobinski,Laurent ChavattePubMed 34298926
- Nutrients 11:2019Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan Sonet et. al.PubMed 31277500
- Cells 8:2019A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA Gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline Vindry et. al.PubMed 31212706
- Environmental toxicology 33:720-7282018Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangliang Tang,Shengde Wu,Lianju Shen,Yi Wei,Xining Cao,Yangcai Wang,Chunlan Long,Yue Zhou,Dian Li,Fangyuan Huang,Bo Liu,Guanghui WeiPubMed 29663635
- Biochimica et biophysica acta. General subjects 1862:2493-25052018Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.
- Analytica chimica acta 1011:11-192018Comparison of analytical methods using enzymatic activity, immunoaffinity and selenium-specific mass spectrometric detection for the quantitation of glutathione peroxidase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan Sonet et. al.PubMed 29475480
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Rat | Predicted | Predicted | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed:11115402, PubMed:36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402).
Alternative names
Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, GPX1
Recommended products
Rabbit Recombinant Monoclonal Glutathione Peroxidase 1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3311
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutathione Peroxidase 1 (GPx1) also known as GSH peroxidase is a selenium-containing enzyme with a mass of approximately 22 kDa. It plays an important role in reducing hydrogen peroxide to water using glutathione as a substrate therefore protecting cells from oxidative damage. GPx1 is widely expressed in many tissues but is found in high concentrations in the liver and erythrocytes. Researchers use antibodies specific to GPx1 often obtained from specialized suppliers to study and quantify this enzyme in various biological samples.
- Biological function summary
The enzyme guards cellular components by neutralizing free radicals. GPx1 is not part of a large complex but functions as a homotetramer. Its activity is essential for preserving the redox balance within the cell preventing cellular damage from reactive oxygen species (ROS). Enzyme-linked immunosorbent assays (ELISA) often measure GPx1 activity to assess oxidative stress levels in research and clinical settings.
- Pathways
GPx1 is important in the glutathione metabolism and cellular antioxidant defense pathways. Within these pathways GPx1 works closely with proteins like glutathione reductase to maintain reduced glutathione levels. This interplay with other antioxidant proteins ensures cellular protection against oxidative damage. Studies focus on GPx1's role in these pathways as it underpins many critical cellular processes maintaining cell health.
- Associated diseases and disorders
Decreased GPx1 activity links to neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases such as Alzheimer's the oxidative stress overwhelms the antioxidant defenses including GPx1. Similarly in cardiovascular disorders a diminished GPx1 activity correlates with increased oxidative stress contributing to disease progression. Researchers also investigate connections between GPx1 and other oxidative stress-related proteins like superoxide dismutase to fully understand GPx1's role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img166403.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
False colour image of Western blot: Anti-Glutathione Peroxidase 1 antibody [EPR3311] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108429 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell line ab266650 (knockout cell lysate Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: GPX1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell line (Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell line ab266650)
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img57573.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 μg)
Lane 3: THP1 cell lysate (20 μg)
Lane 4: HL60 cell lysate (20 μg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
Predicted band size: 22 kDa
![Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--flow-cytometry-intracellular-img37361.jpg "Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
Intracellular Flow Cytometry analysis of THP-1 (human acute monocytic leukemia) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img137060.gif "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: SH SY5Y cell lysate at 10 µg
Lane 3: THP1 cell lysate at 10 µg
Predicted band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137270.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img430448.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: rat skeletal muscle (reference HPA protein expression data).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1: Rat liver tissue lysate at 20 µg
Lane 2: Rat lung tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-ab108429--western-blot-img430448.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
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