Rabbit Recombinant Monoclonal Glutathione Peroxidase 1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (AB247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img166403.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (AB247669)")
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (AB247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img57573.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (AB247669)")
![Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (AB247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--flow-cytometry-intracellular-img37361.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (AB247669)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Expected | Tested | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
1 products for Alternative Version
Target data
Function
Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism (PubMed:11115402).
Alternative names
Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1
Recommended products
Rabbit Recombinant Monoclonal Glutathione Peroxidase 1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Alternative names
Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR3311
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247669 is the carrier-free version of Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img166403.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
False colour image of Western blot: Anti-Glutathione Peroxidase 1 antibody [EPR3311] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell line ab266650 (knockout cell lysate Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: GPX1 knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img57573.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
This data was developed using Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 μg)
Lane 3: THP1 cell lysate (20 μg)
Lane 4: HL60 cell lysate (20 μg)
Lanes 1 and 2: Merged signal (red and green). Green - Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, observed at 22 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429)
Predicted band size: 22 kDa
![Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--flow-cytometry-intracellular-img37361.jpg "Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
Flow Cytometry (Intracellular) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
This data was developed using Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of THP-1 (human acute monocytic leukemia) cells labeling Glutathione Peroxidase 1 with purified Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img137060.gif "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
This data was developed using Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: SH SY5Y cell lysate at 10 µg
Lane 3: THP1 cell lysate at 10 µg
Predicted band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137270.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
This data was developed using Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, the same antibody clone in a different buffer formulation.Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img430448.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
This data was developed using Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: rat skeletal muscle (reference HPA protein expression data).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (Anti-Glutathione Peroxidase 1 antibody [EPR3311] ab108429) at 1/1000 dilution
Lane 1: Rat liver tissue lysate at 20 µg
Lane 2: Rat lung tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: rat skeletal muscle (reference HPA protein expression data).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-1-antibody-epr3311-bsa-and-azide-free-ab247669--western-blot-img430448.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)")
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