AB140130

Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)]

RabMAb
Recombinant
20ul selling size
KO Validated
What is this?

Be the first to review this product! Submit a review

(12 Publications)

Rabbit Recombinant Monoclonal Glutathione Peroxidase 2/GPX2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

Glutathione peroxidase 2, GPx-2, GSHPx-2, Gastrointestinal glutathione peroxidase, Glutathione peroxidase-gastrointestinal, Glutathione peroxidase-related protein 2, Phospholipid hydroperoxide glutathione peroxidase GPX2, GPx-GI, GSHPx-GI, GPRP-2, GPX2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (AB140130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (AB140130)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Glutathione Peroxidase 2/GPX2 with ab140130 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (AB140130)

All lanes:

Western blot - Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (ab140130) at 1/1000 dilution

Lane 1:

Human colon cancer lysate at 10 µg

Lane 2:

Human stomach lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 22 kDa

false

Carrier free

Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8175(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 2 (GPX2) also known as GPX-GI or GPX2 weight belongs to the glutathione peroxidase family. This enzyme plays a mechanical role in reducing hydrogen peroxide by using glutathione which protects cells from oxidative damage. GPX2 is a selenoprotein with a molecular weight of approximately 22 kDa. It is expressed in the gastrointestinal tract particularly in the epithelial cells of the intestines and is also present in other tissues to a lesser extent.

Biological function summary

GPX2 functions as an antioxidant defense mechanism. It works in safeguarding cells by reducing reactive oxygen species and maintaining cellular redox balance. GPX2 is not part of a larger multiprotein complex but works closely with other antioxidant proteins to protect cells. Its expression helps regulate cellular homeostasis and supports tissue integrity especially under oxidative stress conditions.

Pathways

GPX2 continues its relevance in the glutathione metabolism pathway where it cooperates with other glutathione peroxidases and detoxifies peroxides. GPX2 also participates in the oxidative stress response pathway and interacts with proteins like GSSG and GPX1 enhancing the cellular antioxidant capacity. Its activity in these pathways underlines its significant role in maintaining cellular health and integrity by preventing oxidative damage.

GPX2 relates it to colorectal cancer and inflammatory bowel disease. In such conditions GPX2's expression levels are often altered indicating its role in cancer progression and inflammation management. The protein connects to other antioxidant enzymes such as GPX1 and SOD in the context of these diseases with changes in its function potentially impacting disease development and progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 36608588, PubMed : 8428933). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 36608588, PubMed : 8428933). Cannot reduce phosphatidycholine hydroperoxide (PubMed : 8428933).

See full target information Glutathione peroxidase 2

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:456 PubMed40251668

2025

GPX2 inhibition enhances antitumor efficacy of lenvatinib via promoting immunogenic cell death in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzheng Tan,Lei Yang,Tao Xu,Qingbin Wang,Meiyuan Huang,Zhijian Zhao,Xun Chen,Caixi Tang,Wenliang Tan

Translational oncology 41:101870 PubMed38262108

2024

BRMS1L confers anticancer activity in non-small cell lung cancer by transcriptionally inducing a redox imbalance in the GPX2-ROS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Penglong Cao,Juebin Gu,Mulin Liu,Yingxin Wang,Mingying Chen,Yizhu Jiang,Xiaoyan Wang,Siqi Zhu,Xue Gao,Shijun Li

Oncogene 42:1994-2006 PubMed37138031

2023

Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Handong Xu,Can Hu,Yi Wang,Yunfu Shi,Li Yuan,Jingli Xu,Yanqiang Zhang,Jiahui Chen,Qin Wei,Jiangjiang Qin,Zhiyuan Xu,Xiangdong Cheng

PeerJ 10:e14263 PubMed36312753

2022

GPX2 predicts recurrence-free survival and triggers the Wnt/β-catenin/EMT pathway in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Yang,Xudong Zhu,Yang Shen,Qi He,Yuan Qin,Yiqun Shao,Lin Yuan,Hesong Ye

Oncology reports 48: PubMed36222298

2022

Glutathione peroxidase 2 overexpression promotes malignant progression and cisplatin resistance of KRAS‑mutated lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Wang,Xu Chen,Guang Fu,Mingjian Ge

Cancer research 82:3486-3498 PubMed35916672

2022

Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate.

Applications

Unspecified application

Species

Unspecified reactive species

Connor S R Jankowski,Joshua D Rabinowitz

Molecular cancer 21:43 PubMed35144642

2022

Methyltransferase like 7B is a potential therapeutic target for reversing EGFR-TKIs resistance in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huibin Song,Dongcheng Liu,Lingwei Wang,Kaisheng Liu,Chen Chen,Le Wang,Yi Ren,Bing Ju,Fuhua Zhong,Xingyu Jiang,Guangsuo Wang,Zhe-Sheng Chen,Chang Zou

Frontiers in cell and developmental biology 9:684259 PubMed34307361

2021

Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Yan Zhou,Lizhe Zhu,Huan Gao,Jin Yang

Oxidative medicine and cellular longevity 2020:7370157 PubMed32215178

2020

Elevated Glutathione Peroxidase 2 Expression Promotes Cisplatin Resistance in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

He Du,Bi Chen,Nan-Lin Jiao,Yan-Hua Liu,San-Yuan Sun,You-Wei Zhang

OncoTargets and therapy 12:6639-6651 PubMed31695405

2019

GPX2 suppression of HO stress regulates cervical cancer metastasis and apoptosis via activation of the β-catenin-WNT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxin Wang,Penglong Cao,Mohammed Alshwmi,Nan Jiang,Zhen Xiao,Fengquan Jiang,Juebin Gu,Xiaonan Wang,Xiaoye Sun,Shijun Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (AB140130)

Western blot - Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (AB140130)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise