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AB289369

Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free

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Rabbit Recombinant Monoclonal Glutathione Peroxidase 2/GPX2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human, Dog samples. Immunogen corresponding to Synthetic Peptide within Human Glutathione peroxidase 2.

View Alternative Names

Glutathione peroxidase 2, GPx-2, GSHPx-2, Gastrointestinal glutathione peroxidase, Glutathione peroxidase-gastrointestinal, Glutathione peroxidase-related protein 2, Phospholipid hydroperoxide glutathione peroxidase GPX2, GPx-GI, GSHPx-GI, GPRP-2, GPX2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded mouse colon.
GPX2 stained by GPX2 antibody [HL125] (ab289369) diluted at 1 : 200.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

Paraffin-embedded rat tissue stained for Glutathione Peroxidase 2/GPX2 protein with ab289369 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

Paraffin-embedded dog kidney tissue stained for Glutathione Peroxidase 2/GPX2 protein at cytoplasm with ab289369 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL125

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse, Dog

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Glutathione peroxidase 2. The exact immunogen used to generate this antibody is proprietary information.

P18283

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "500-3000 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 2 (GPX2) also known as GPX-GI or GPX2 weight belongs to the glutathione peroxidase family. This enzyme plays a mechanical role in reducing hydrogen peroxide by using glutathione which protects cells from oxidative damage. GPX2 is a selenoprotein with a molecular weight of approximately 22 kDa. It is expressed in the gastrointestinal tract particularly in the epithelial cells of the intestines and is also present in other tissues to a lesser extent.
Biological function summary

GPX2 functions as an antioxidant defense mechanism. It works in safeguarding cells by reducing reactive oxygen species and maintaining cellular redox balance. GPX2 is not part of a larger multiprotein complex but works closely with other antioxidant proteins to protect cells. Its expression helps regulate cellular homeostasis and supports tissue integrity especially under oxidative stress conditions.

Pathways

GPX2 continues its relevance in the glutathione metabolism pathway where it cooperates with other glutathione peroxidases and detoxifies peroxides. GPX2 also participates in the oxidative stress response pathway and interacts with proteins like GSSG and GPX1 enhancing the cellular antioxidant capacity. Its activity in these pathways underlines its significant role in maintaining cellular health and integrity by preventing oxidative damage.

GPX2 relates it to colorectal cancer and inflammatory bowel disease. In such conditions GPX2's expression levels are often altered indicating its role in cancer progression and inflammation management. The protein connects to other antioxidant enzymes such as GPX1 and SOD in the context of these diseases with changes in its function potentially impacting disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 36608588, PubMed : 8428933). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 36608588, PubMed : 8428933). Cannot reduce phosphatidycholine hydroperoxide (PubMed : 8428933).
See full target information Glutathione peroxidase 2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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