Anti-GPx-3 + GPx-5 antibody [EPR19951-136] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPx-3 + GPx-5 antibody [EPR19951-136] - BSA and Azide free (AB275969)

This data was developed using ab275965, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GPx-3 + GPx-5 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on proximal tubules of human kidney. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPx-3 + GPx-5 antibody [EPR19951-136] - BSA and Azide free (AB275969)

This data was developed using ab275965, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GPx-3 + GPx-5 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of human seminiferous tubules. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-GPx-3 + GPx-5 antibody [EPR19951-136] - BSA and Azide free (AB275969)

This data was developed using ab275965, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression pattern is consistent with what has been described in the literature (PMID : 20015939).

Low expression : human liver (PMID : 9820194, 15294905).

Exposure time : 92 seconds.

All lanes:

Western blot - Anti-GPx-3 + GPx-5 antibody [EPR19951-136] (<a href='/en-us/products/primary-antibodies/glutathione-peroxidase-3-gpx-3-antibody-epr19951-136-ab275965'>ab275965</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 22 kDa,24 kDa

false

• WB

Lab

Western blot - Anti-GPx-3 + GPx-5 antibody [EPR19951-136] - BSA and Azide free (AB275969)

This data was developed using ab275965, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression pattern is consistent with what has been described in the literature (PMID : 20015939).

Exposure time : Lane 1 : 37 seconds Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-GPx-3 + GPx-5 antibody [EPR19951-136] (<a href='/en-us/products/primary-antibodies/glutathione-peroxidase-3-gpx-3-antibody-epr19951-136-ab275965'>ab275965</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 26 kDa

Observed band size: 24 kDa

false