Skip to main content

Rabbit Recombinant Monoclonal Glutathione Peroxidase 3/GPx-3 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 8 publications.


Images

Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (AB256470), expandable thumbnail
  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (AB256470), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (AB256470), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (AB256470), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (AB256470), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Mouse
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Alternative names

Glutathione peroxidase 3, GPx-3, GSHPx-3, Plasma glutathione peroxidase, GPx-P, GSHPx-P, Gpx3

Recommended products

Rabbit Recombinant Monoclonal Glutathione Peroxidase 3/GPx-3 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22815-112
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutathione Peroxidase 3 also known as GPx-3 or 3GPx is an antioxidant enzyme important for protecting cells from oxidative damage by reducing hydrogen peroxide and organic hydroperoxides. It is a member of the glutathione peroxidase family and has a molecular mass of approximately 23 kDa. GPx-3 is expressed mainly in the kidney and subsequently released into the bloodstream playing an important role in its extracellular antioxidant defense mechanism.

Biological function summary

GPx-3 helps maintain cellular redox balance and is involved in detoxifying harmful oxidative agents. It does not form part of any larger complex but works independently to reduce peroxides with the help of glutathione an important reducing agent. GPx-3 has significant activities in plasma and extracellular fluids indicating its role in protecting the body from oxidative stress away from cellular environments.

Pathways

GPx-3 functions in the glutathione metabolic pathway and the defense system against oxidative stress. It works together with proteins such as glutathione reductase to recycle oxidized glutathione back to its reduced form allowing continuous protection against reactive oxygen species. This pathway is essential for maintaining balance between oxidative stress and antioxidant defense in the organism.

Associated diseases and disorders

GPx-3 has been linked to cardiovascular diseases and certain types of cancer. Low levels of GPx-3 in plasma have been associated with increased risk of thrombosis as antioxidant defenses are compromised. In cancer studies altered GPx-3 expression is often observed thought to affect the progression of disease by changing the cellular redox environment. Other proteins that interact with GPx-3 during disease conditions include thioredoxin and catalase both influencing and being regulated by redox states in diseased tissues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20015939).

    The 22-KDa band represents another Gpx3 isoform.

    Negative control: Mouse liver (PMID: 15294905).

    All lanes: Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470) at 1/1000 dilution

    Lane 1: Mouse kidney lysate at 20 µg

    Lane 2: Mouse liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa

    Observed band size: 22-24 kDa

    Exposure time: 48s

  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20015939).

    All lanes: Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470) at 1/1000 dilution

    All lanes: Rat kidney lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 26 kDa

    Observed band size: 24 kDa

    Exposure time: 26s

  • Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on rat kidney (PMID: 21493731) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on mouse kidney (PMID: 21493731) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat lung (PMID: 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat kidney (PMID: 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse lung (PMID: 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse kidney (PMID: 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com